ABSTRACT
Background: Rostelluria procumbens is a medicinal plant used traditionally in the treatment of asthma, cough and constipation and as an antioxidant etc. it is rich in phytochemical compounds, which are responsible for its biological properties. The present study focused on evaluation of hepatoprotective activity of methanolic extract of R. procumbens leaf in carbon tetrachloride (CCl4) induced hepatotoxocity in rats. Methods: In this study, 30 wistar rats were used and grouped into 6 each group contain 6 rats. In this study, CCl4 is used as hepatotoxin. Four groups were treated with CCl4 and taken as disease control, standard, and two test groups. One group was taken as control treated with saline. Blood samples were collected and estimated serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase and total bilirubin, which are key markers of liver function. The rats were sacrificed and livers were isolated and histopatological studies carried out. Results: On oral administration of methanolic leaves extract of R. procumbens to ethanol intoxicated, rats resulted in significant restoration of enzyme levels and also silymarin at a dose of 25 mg/kg. The reversal of increased serum enzymes in ethanol-induced liver damage by the extract may be due to the prevention of leakage of intracellular enzymes by its membrane stabilizing activity. Conclusion: The results confirm that R. procumbens have hepatoprotective activity against CCl4 induced hepatotoxicity and significant hepatoprotection seen at 500 mg/kg dose.
ABSTRACT
Abstract : Present study was carried out to evaluate hepatotoxicity of nimesulide by single dose and seven days administration in sub therapeutic, therapeutic and supra therapeutic doses in litters of rat. Single dose and seven days administration hepatotoxicity studies of nimesulide were carried out in litters of rat of either sex. They were further subdivided into sub therapeutic (20 mg/kg), therapeutic (30 mg/kg) and supra therapeutic (100 mg/kg) groups. Effect of nimesulide on liver functions were analysed by serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and histopathological examination of liver through scoring system. Histopathological changes in liver were observed in therapeutic and supra therapeutic doses in single dose groups and sub therapeutic, therapeutic and supra therapeutic doses in seven days groups. In single dose of nimesulide in litters, there were significant increases in biochemical parameters (p< 0.05) in supratherapeutic doses. However, in seven days studies of nimesulide in litters, there were significant increases in biochemical parameters (p< 0.05) in therapeutic and supratherapeutic doses. The present study indicates that nimesulide causes significant hepatotoxicity in litters of rat.