Observations on the Therapeutic Effect of Combined Moxibustion and Medicine on the Clinical Remission Stage of Bronchial Asthma / 上海针灸杂志

Objective To investigate the clinical efficacy of gentle moxibustion plus oral Gushen Dingchuan pills in treating the clinical remission stage of bronchial asthma.Methods Ninety patients with bronchial asthma in the clinical remission stage were randomized to groups A,B and C,30 cases each.group A received moxa-stick gentle moxibustion;group B,oral administration of Gushen Dingchuan pills;group C,moxa-stick gentle moxibustion plus oral administration of Gushen Dingchuan pills.The course of treatment was three months in all the three groups.The TCM symptom score and the Asthma Control Test (ACT) score were recorded and serum immunoglobulin E (IgE) content was measured in the two groups before and after treatment.The clinical therapeutic effects were compared between the groups.Results The total efficacy rate was 76.7％ in group A,80.0％ in group B and 93.3％ in group C.there was a statistically significant difference in the total efficacy rate between group C and group A or B (P＜0.05).There were statistically significant pre-/post-treatment differences in the TCM symptom score,the ACT score and serum IgE content in all the three groups (P＜0.01).There were statistically significant post-treatment differences in the TCM symptom score,the ACT score and serum IgE content between between group C and group A or B (P＜0.01,P＜0.05).Conclusion Gentle moxibustion plus oral Gushen Dingchuan pills can improve immunologic function in patients with bronchial asthma in the clinical remission stage.

Histopathology of the temporal artery in young ischemic stroke patients with elevated serum immunoglobu-lin E / 医学研究生学报

Objective Ischemic stroke with elevated serum immunoglobulin E ( IgE) in some young patients is regarded as cerebral vasculitis clinically though without sufficient pathological evidence .This study was to investigate the characteristics of vascular lesions in these patients by temporal artery biopsy . Methods We performed histopathologic examinations on the temporal arteries of 32 young ischemic stroke patients with unknown etiology , 16 with normal and the other 16 with elevated serum IgE .We observed inflammatory cells infiltration and mast cells by HE staining and toluidine blue stai-ning respectively and determined the expressions of matrix metalloproteinase -9 (MMP-9), monocyte chemotaxis protein -1 (MCP-1) and serum IgE by immunohistochemistry . Results Compared with the patients with normal IgE , those of the elevated IgE group showed a significantly higher rate of inflammatory cells infiltration (12.5%vs 62.5%, P0.05).Nor was any signifi-cant difference observed in the number of the mast cells between the normal and elevated IgE groups (2.8 ±1.5 vs 3.6 ±2.3, P>0.05). Conclusion The infiltration and necrosis of inflammatory cells and fibrin exudation in the temporal artery of the young pa-tient with elevated serum IgE are likely to be the manifestations of vasculitis , and MCP-1 may play a role in the pathogenesis of the disease.

A study on human leukocyte antigen class I molecules in paediatric bronchial asthma.

Childhood asthma, often associated with atopy, is more common in boys and may persist throughout life in 50% of cases. This case-control study was carried out to examine if any association of paediatric bronchial asthma with human leukocyte antigen (HLA) class I antigens. Thirty-six children with bronchial asthma diagnosed on basis of Global Initiative for Asthma (GINA) criteria and an equal number of healthy controls without history of bronchial asthma were studied. Low resolution HLA- ABC typing was performed by sequence specific primers (SSP) and the frequency of HLA–ABC antigens in the two groups was compared. Total serum immunoglobulin E (IgE) estimation was done as a marker of atopy by ELISA. The study included 24 boys and 12 girls aged 13 months to 11 yrs, of which 16 (44%) had positive family history. Serum IgE levels were elevated in 20 (55%) of the cases and 33% of controls with peak values of 4877 and 627 IU/ml, respectively. No statistically significant correlation was observed between childhood asthma and HLA class I antigens, however, a statistically significant correlation was observed between serum IgE levels and asthma, which was elevated in cases, as compared to normal population. Serum IgE levels did not show a linear trend, in that a direct correlation with the severity of disease was not observed.

Serum immunoglobulin E (IgE) levels and dietary intake of Korean infants and young children with atopic dermatitis

Atopic dermatitis (AD) has become a serious epidemic in Korean children. We aimed to investigate the association between vitamin C, E and other nutrients, and serum total IgE/specific IgE levels in children with AD. A total of 119 children (0-24 mo) diagnosed with AD were recruited for this cross-sectional study from a medical center in Seoul. A 24 h recall was used to assess dietary intakes. Serum total and six food-allergen specific IgE levels were measured by CAP-FEIA. Serum vitamin E was also measured but only in 25 out of the total 119 participants. Multiple linear regression analysis was performed to estimate the coefficients between serum IgE levels and dietary intake as well as serum vitamin E. Serum vitamin E levels showed a significantly inverse association with serum total IgE and all specific IgE levels (P < 0.05). Fat intake was inversely related with specific-IgEs for egg whites, milk, buck wheat, soy, and peanuts (P < 0.05). Positive associations were found between carbohydrate (CHO) intake and total IgE and specific IgEs to egg whites, milk, soy, and peanuts (P < 0.05). Vitamin C, E and n-3/n-6 fatty acids were not related with serum total IgE and specific IgE levels except for the association between buck wheat and vitamin E. In addition, there were no significant differences between males and females in dietary intake and serum IgE levels by student's t-test. Although dietary vitamin E showed no association with serum IgE levels, serum vitamin E drew a significant inverse relationship with serum IgE levels. The evidence seems to suggest that vitamin E may possibly lower total and specific-IgEs in children with AD, and that it is important to maintain a relatively high serum vitamin E level in children with AD.

Significance of Detection of Allergen-Specific Serum Immunoglobulin E in Children with Asthma / 实用儿科临床杂志

Objective To investigate the positive rates of food allergen (fx5),mixed molds (mx1),phadiatop and house dust mites (d1) allergen-specific serum immunoglobulin E(sIgE) in children with asthma or cough variant asthma (CVA).Their age distribution characte-ristics were also compared and analysed.Methods Blood samples of 597 asthmatic children aging from 6 months to 16 years old were applied to fx5,mx1,pha-diatop and d1 test by using fluoroenzyme-immunometricassay,UniCAP100.The positive rate of fx5,mx1,phadiatop and d1 were compared.Age distribution characteristics of children with positive sIgE were analysed.Results 1.The positive rate of fx5,mx1,pha-diatop and d1 were 34.6%,30.0%,35.2% and 23.5%,respectively.2.The highest positive rate of phadiatop and d1 were highest in children of 6-16 age group(67.8% and 46.3%);The positive rate of fx5,mx1 peaked in children of 2 years and 5 years separately(44.1% and 53.6%);3.Among 181 children with mx1 positivity 90 cases manifested phadiatop negativity.4.Sixty-five percent of children with clinically diagnosed asthma or CVA presented positivity in test in fx5,mx1 or phadiatop at least.Conclusions Two thirds asthma and CVA patients exist sIgE.Asthmatic infants mainly manifest food allergen sensitization.The positive of phadiatop increased with age.Positivity of mx1 increased with age ≤5 years old in children,and it decreases in older children(≥6 years old).Mx1 is one of the main allergens in asthmatic children.

Changes of Eosinophils Count an Total IgE Levels in Patients With Myocoplasm Pneumoniae / 中国医师杂志

Objective To investigate the number of eosinophils in peripheral blood and total serum immunoglobulin E levels from children during infection of mycoplasma pneumoniae,which may elucidate what mycoplasma pneumoniae plays a role in persistent cough and asthma onset.Methods The number of eosinophils in peripheral blood was counted under microscope and total serum immunoglobulin E levels were determined by ELISA in 20 children with mycoplasmal infection ,30 patients with asthma and 25 control subjects.Results The number of eosinophils and total serum immunoglobulin E levels during mycoplasmal infection were significantly higher than in control group,and lower than in asthma.Conclusion The high eosinophil counts and total serum immunoglobulin E induced by mycoplasma pnemoniae play a key role in persistent cough and asthma attack.