ABSTRACT
Objective: To study the constituents and metabolites in rat brain after ig administration of Xueshuan Xinmaining Tablet (XXT) based on the serum medicinal chemistry. Methods: The acute blood stasis rat model was replicated by ice bath-adrenalin method, and XXT [1.4 (g∙kg-1∙d-1)] was administered orally for 8 d. The brain samples were collected and pretreated after ig administration for the last time. Then the absorbed prototype constituents and their metabolites in rats plasma were rapidly analyzed and identified by combination of ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and multivariate statistical analysis. Results: Finally, a total of 11 absorbed prototype constituents and three metabolites were identified, including phenanthrene, bufonis venenum oxadiene, and other structural types of compounds. These constituents absorbed into brain may be the potential bioactive components in XXT. Conclusion: The establishment of UPLC-Q-TOF/MS analysis method explained comprehensively the brain migration component of XXT, and provided scientific basis for clarifying its substance basis of pharmacodynamics of this large variety of Chinese medicine.
ABSTRACT
The preparation of Chinese materia medica (CMM) possesses the characteristic of holistic function involving multi-component, multi-target, and multi-link. This characters make it difficult to optimize the preparation process and construct the quality standards of CMM. Thus process building and quality evaluation system based on correctly identifying the components that closely related to the efficacy is a key scientific issue in the preparation process design and quality control of CMM. In this paper, a more comprehensive analysis on the feasibility of different research methods was carried out, including the spectral-efficiency relationships, pharmacokinetics, serum medicinal chemistry and serum pharmacology of drugs, the effect of integration effect correlation analysis, virtual screening, and comparative analysis of knock-in/knock-out. The purpose of this article is to provide the reference for identifying the bioactive marker group which is used for the CMM preparation process and quality evaluation.