ABSTRACT
Objective:The purpose of our experiment was to use CT to observe the opening of BBB at the early stage of severely scalded rabbits. Methods:Healthy male New Zealand white rabbits were used to establish 30% and 50% TBSA Ⅲ?scalded model. Using high resolution spiral CT (SCT), the brain parenchyma were observed when plain CT scan and contrast-enhanced CT were performed. Results:CT image changes were found in 4 cases at 6 hour after scald of 30% TBSA Ⅲ?scalded rabbits, while CT image changes were found in all cases at 6 hour after scald of 50% TBSA Ⅲ?scalded rabbits. Conclusion:The larger TBSA is Ⅲ?scalded, the earlier BBB is opening . BBB of 50% TBSA Ⅲ?scalded rabbits are wider than that of 30% TBSA Ⅲ?scalded rabbits
ABSTRACT
Objective To explore the image characteristics of the opening of the blood brain barrier (BBB) at the early stages of severely scalded rabbits. Methods Twenty-six rabbits inflicted with 50% total burn surface area (TBSA) of Ⅲ degree were randomized into 7 groups: pre-scald, post-scald at 1, 2, 3, 4, 5, and 6 h groups. The spiral CT (SCT) and MRI were used to observe the image changes during the corresponding phases. Samples from the anterior cortex, temporal lobe, posterior cortex, cerebellum, and brain stem were examined by light and transmission electron microscopy (TEM), respectively. Results The pathological features showed that neurons and oligodendrocytes swelled lightly and blood vessels were congested and dilated at 3-4 h after scald. CT image changes were found at 5 h after scald and became more remarkable at 6 h after scald. MRI image changes could not be found within 6 h after scald. Conclusion The opening of BBB is earlier than the formation of edema. Enhanced CT plays an important role in manifesting the opening of BBB at the early stages of severely scalded rabbits.
ABSTRACT
Objective To investigate the changes of the expression of HSP90? in the liver of severely scalded rats at the early stage of stress Methods The expression levels of HSP90? in the liver of rats at different time points after severe scald were detected by Western blotting and then the expression of HSP90? in the nucleuses was observed by immunohistochemistry Results The expression of HSP90? significantly increased in the liver of rats after severe scald, especially during 12～48 h Abnormal high expression of HSP90? in the nucleuses around was found at 48 h after scald Conclusion The abnormal high expression of HSP90? due to severe scald may be one of the important causes resulting in the disorder of glucocorticoid receptor at the early stage of stress after severe trauma.