ABSTRACT
OBJECTIVE: To establish an LC-MS/MS method to determine eleven components(paeoniflorin, acteoside, ferulic acid, naringin, salvianolic acid B, lobetyolin, hesperidin, liquiritin, isoliquiritoside, liquiritigenin, isoliquiritigenin) in Shengan Guanxin Mixture(traditional Chinese medicines) simultaneously. METHODS: The HPLC analysis was performed on an Agilent SB-C18 column(2.1 mm × 50 mm, 1.8 μm) with gradient elution of 0.1% formic acid and methanol containing 0.1% formic acid, the flow rate was 0.3 mL · min, and the column temperature was room temperature. Electrospray ionization (ESI) source was used. Quantitation was performed by using selected multiple reaction monitoring (MRM) mode. RESULTS: The method had good linearity in the ranges of 0.7580-30.32 μg · mL-1 (r =0.9949) for paeoniflorin, 0.3040-12.16 μg · mL-1 (r =0.9995) for acteoside, 0.2480-9.920 μg · mL-1 (r = 0.9969) for ferulic acid, 0.5035-20.14 μg · mL-1 (r = 0.9983) for naringin, 0.9100-36.40 μg · mL-1 (r = 0.9994) for salvianolic acid B, 0.0975-3.900 μg · mL-1 (r = 0.9989) for lobetyolin, 0.9965-39.86 μg · mL-1(r = 0.9982) for hesperidin, 0.2535-10.14 μg · mL-1 (r =0.9975) for liquiritin, 0.2480-9.920 μg · mL-1 (r = 0.9967) for isoliquiritoside, 0.2770-11.08 μg · mL-1 (r = 0.9950) for liquiritigenin, and 0.1530-6.120 μg · mL-1 (r = 0.9928) for isoliquiritigenin. The average recoveries of the eleven active components were 98.47%-101.91% with RSDs of less than 2.5% (n=6). CONCLUSION: The method is convenient, rapid, sensitive and reliable. It may provide a basis for the quality control of Shengan Guanxin Mixture.