ABSTRACT
Objective: To explore the prophylactic and curative effects of Shengxue Pills on formaldehyde-induced cell damage of lung, liver, spleen, bone marrow, and peripheral blood lymphocytes in mice and the mechanisms. Methods: The mice were randomly divided into five groups: control, Shengxue Pill prophylaxis, Shengxue Pill therapy, and two model groups. ELISA was used to detect hydroxyl radicals, superoxide anion radical, and ornithine decarboxylase (ODC) activities in the lung, liver, and spleen cells. Bone marrow cells and peripheral blood lymphocyte micronucleus rate were observed with high power lens. Results: Hydroxyl radicals, superoxide anion radicals, and ODS activities in the cells of the organs in mice were investigated, and the number of bone marrow cells and peripheral blood lymphocyte micronucleus rate in mice in the prophylacitc and therapeutic groups were significantly greater than those in the model groups, respectively as well as in the normal group. Conclusion: Shengxue Pills could prevent and cure the formaldehyde-induced cell damages of the lung, liver, spleen, bone marrow, and peripheral blood lymphocytes of mice.
ABSTRACT
Objective: To study the prevention and rehabilitation of Shengxue Pills on liver, spleen, bone marrow cells, peripheral blood lymphocyte of mice injured by low-dose radiation and their mechanisms. Methods: Mice were randomly divided into five groups: control, Shengxue Pills prevention, Shengxue Pills rehabilitation, model 1 (corresponding to rehabilitation), and model 2 (corresponding to prevention) groups. Using the ELISA method to detect the activity of hydroxyl radical, superoxide anion radical, and ornithine decarboxylase (ODC) in liver and spleen cells. The bone marrow cells and peripheral lymphocyte micronucleus rate were observed under high power lens. Results: Compared with control group, the amounts of hydroxyl radical and superoxide anion radical in the two model groups were obviously increased. Compared with the corresponding model groups, the above indexes in prevention group and rehabilitation group were significantly decreased. Compared with the control group, the activity of ODC in mice of the two model groups was obviously enhanced, but bone marrow cells and peripheral lymphocyte micronucleus rate were increased. Compared with the corresponding model groups, the activity of ODC in mice of prevention and rehabilitation groups was weakened, and bone marrow cells and peripheral lymphocyte micronucleus rate were decreased. But each index of spleen cells showed no significant difference. Conclusion: Shengxue Pills could effectively prevent and rehabilitate the damage in liver cells, peripheral blood lymphocytes, and bone marrow cells of mice injured by low-dose radiation.