ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of matrine,linarin,berberine hydrochloride and osthole in Shenju lotion. METHODS:HPLC was performed on the column of Dionex C18 with mobile phase of acetonitrle-0.1% triethylamine(pH was adjusted to 7.2 with phosphoric)(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 30 ℃,and injection volume was 20 μl. RESULTS:The linear range was 1.400-8.400 μg for matrine (r=0.999 4),0.152-0.912 μg for linarin (r=0.999 6),0.248-1.488 μg for berberine hydrochloride (r=0.999 9) and 0.128-0.768 μg for osthole (r=0.999 4);limit of quantitation was 84.85,3.15,15.03,7.76 ng,limit of detection was 24.13, 0.89,4.27,2.20 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 96.29%-99.06%(RSD=1.10%,n=9),96.49%-98.27%(RSD=0.69%,n=9),99.26%-101.13%(RSD=0.62%,n=9) and 96.27%-98.64%(RSD=0.89%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the simultaneous determination of matrine,linarin,berberine hydrochloride and osthole in Shenju lotion.
ABSTRACT
Objective To investigate the in vitro effect of ShenJu lotion agaisnt Trichomonas vaginalis in order to provide clues for its clinical application. Methods ShenJu lotion was formulated into different concentrations(400.00,200. 00,100.00,50.00,25.00,12.50,6.25 mg·mL-1 )with liver extracts.The morphology and movement of Trichomonas vaginalis treated with different concentrations of ShenJu lotion in vitro for 2,4,6,8,12,or 24 h were observed and the mortality was calculated.The effects against Trichomonas vaginalis were compared between Jieeryin lotion and ShenJu lotion at the same concentrations. Results ShenJu lotion could significantly inhibit and kill clinical isolates of Trichomonas vaginalis in vitro and the lowest effective concentration was 50.00 mg·mL-1 .There was no significant difference in the mortality between ShenJu lotion and Jieeryin lotion(P>0.05). Conclusion ShenJu lotion has obvious effects against Trichomonas vaginalis.
ABSTRACT
Objective To study the antibacterial effect of Shenju lotion in vitro. Methods The diameter of inhibition zone was determined by paper-disc agar-diffusion method. Minimum inhibitory concentration ( MIC) and Minimum bactericidal concentration ( MBC ) was determined by culture medium dilution methodand agar medium plate method, respectively. Antibacterial effect was compared between Shenju lotion and city sale of the gynecological lotion. Results Inhibitory effects of Shenju lotion on 5 pathogenic strains were significantly better than that of city sale of the gynecological lotion at the same concentrations (P<0. 05). MIC of Shenju lotion on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Candida albicans was 33. 75, 67. 5, 67. 5, 67. 5 and 33. 75 mg ·mL-1 , respectively. MBC of Shenju lotion on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Candida albicans was 33. 75, 67. 5, 67. 5, 135 and 33. 75 mg ·mL-1 , respectively. Conclusion Shenju lotion has obvious bacteriostasis and sterilization effect.