ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Shugan Pills (Toosendan Fructus,Corydalis Rhizoma,Paeoniae Radix Alba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Paeoniflorin,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 0.040 44-1.011,0.045 20-1.130,0.047 08-1.177 and 0.047 24-1.181 μg (r =0.999 9),whose average recoveries were 100.5％,98.6％,98.4％ and 101.3％ with the RSDs of 1.5％,0.9％,1.2％ and 1.1％,respectively.The contents of various constituents in the samples with three dosage forms from seven manufacturers showed obvious differences.CONCLUSION We should pay attention to the uneven quality of Shugan Pills.
ABSTRACT
Objective:To determine aflatoxin B1 , B2 , G1 and G2 in Shugan pills by HPLC and evaluate their safety. Methods:The product was analyzed by HPLC using a Shiseido C18 column(150 mm × 4. 6 mm,5 μm)with methanol-acetonitrile-water (10 :30 :60) as the mobile phase. An ultraviolet lamp was derivative at 254 nm, and the fluorescence detection was performed with the excitation wavelength at 360 nm and the emission wavelength at 450nm. The samples from nation-wide market were detected by the method. Re-sults:Good linear relationships were obtained within the range of 10-50 pg for aflatoxin B1 and G1 , and 3-15 pg for aflatoxin B2 and G2 . The recovery of aflatoxin B1 , B2 , G1 and G2 was 89. 9%,87. 9%,86. 1% and 87. 1%,respectively with all the RSD below 3. 0%. The results of all samples were satisfactory, and the qualification rate of the samples was 100%, indicating that the samples were safe. Conclusion:The developed method is convenient and accurate, which can be applied in the quantitative determination of Shugan pills.
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Shugan Pills (Toosendan Fructus,Corydalis Rhizoma,Paeoniae Radix Alba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Paeoniflorin,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 0.040 44-1.011,0.045 20-1.130,0.047 08-1.177 and 0.047 24-1.181 μg (r =0.999 9),whose average recoveries were 100.5％,98.6％,98.4％ and 101.3％ with the RSDs of 1.5％,0.9％,1.2％ and 1.1％,respectively.The contents of various constituents in the samples with three dosage forms from seven manufacturers showed obvious differences.CONCLUSION We should pay attention to the uneven quality of Shugan Pills.
ABSTRACT
Objective:To determine aflatoxin B1 , B2 , G1 and G2 in Shugan pills by HPLC and evaluate their safety. Methods:The product was analyzed by HPLC using a Shiseido C18 column(150 mm × 4. 6 mm,5 μm)with methanol-acetonitrile-water (10 :30 :60) as the mobile phase. An ultraviolet lamp was derivative at 254 nm, and the fluorescence detection was performed with the excitation wavelength at 360 nm and the emission wavelength at 450nm. The samples from nation-wide market were detected by the method. Re-sults:Good linear relationships were obtained within the range of 10-50 pg for aflatoxin B1 and G1 , and 3-15 pg for aflatoxin B2 and G2 . The recovery of aflatoxin B1 , B2 , G1 and G2 was 89. 9%,87. 9%,86. 1% and 87. 1%,respectively with all the RSD below 3. 0%. The results of all samples were satisfactory, and the qualification rate of the samples was 100%, indicating that the samples were safe. Conclusion:The developed method is convenient and accurate, which can be applied in the quantitative determination of Shugan pills.
ABSTRACT
Objective:To establish the high performance liquid chromatography-fingerprints( HPLC-FPS)of Chaihu Shugan pills. Methods:The analysis was performed on a Zirchrom Kromasil C18 column with the mobile phase of methanol-water(containing 0. 05%phosphoric acid)with gradient elution at a flow-rate of 0. 8 ml·min-1 . The detection wavelength was set at 294 nm and the column temperature was 30℃. Results:With baicalin as the reference substance,there were 24 common peaks in the fingerprints to establish the fingerprint common pattern. The similarity among the 16 batches of samples was calculated,and the results showed it was within the range of 0. 690-0. 993,suggesting the differences among the samples from different factories. Conclusion:The establishment of the fingerprints provides a simple and practical analysis method for more comprehensive regulation of medicinal material sources,effective quality monitoring of the products and the assurance of effective and safe clinical application.
ABSTRACT
AIM: To study the action of Shugan Pills (SHGP) (Fructus Toosendan, Rhizoma corydalis, Radix Paloniae Albe, Radix Aucklandiae, Cortex Magnoliae Officinalis, Fructus Aurantii, etc.) on treatment of epigastralgia. METHODS: The analgesic effect was tested by the hot plate test and writhing method. The anti gastric ulcer action of SHGP was observed on the gastric ulcer induced by water immersion stress, reserpine and absolute ethyl alcohol. The effect on gastric secretion in rats was studied with pylorus ligation. The effect on gastrointestinal motility was observed by determination of gastric emptying and small intestinal propulsion ability in mouse. RESULTS: SHGP enhanced hot pain threhshold and decreased the number of twisting body in mouse. SHGP markedly inhibited gastric ulcer induced by water immersion stress, reserpine and absolute ethyl alcohol. SHGP significantly inhibited the secretion of gastric acid and pepsin and promoted the secretion of gastric mucus. SHGP markedly delayed gastric emptying in normal mouse and when gastric emptying and small intestinal propulsion was stimulated by neostigmine. SHGP faintly strengthed the effect of atropine on inhibition of gastrointestinal motility. CONCLUSION: Shugan Pills could have analgesic effect, anti gastric ulcer action, it inhibited the secrection of gastric juice and the activity of gastric smooth muscle. These effects might be the pharmacological mechanisms of SHGP on treatment of epigastralgia.