ABSTRACT
Objective To derive and empirical validate probability of exclusion in grand-parental double single-parentage cases (PEGDS). Methods Firstly, an exact definition of PEGDSwas established, and PEGDSformula was derived based on the definition. Then the values of formula were calculated in 19 STR loci. Secondly, the simulation values of PEGDSwere counted through multi-group simulation experiments in 19 STR loci. Finally PEGDSformula was empirically validated by correlation method between the values of formula and simulation. Results PEGDSformula was all in accord with the simulation experiments. Cumulative probability of exclusion in grand-parental double single-parentage cases (CPEGDS) in AGCU EX20 system was 1-3.10310×10-9. The system effectiveness in grand-parental double single-parentage cases was a little more than trio cases, and was far more than duo cases. Conclusion PEGDSformula in this paper could be applied to grand-parental double single-parentage cases.
ABSTRACT
To establish a calculation method of paternity probability in cases of absence of mother. Calculating the accumulated non-father exclusion probability of multiple polymorphic DNA loci was performed. The results showed that in cases of inheritance with Mendelian Law, through testing 8 or more polymorphic DNA loci, the paternity probability may reach 0. 9990 or more. In cases of paternity exclusion, exclusion 3 or more than 3 loci were required. For the purpose of identifying paternity in cases of absence of mother, more than 8 polymorphic DNA loci must be tested. The paternity probability must be more than 0. 9990 in cases of paternity inclusion and in cases of paternity exclusion, 3 or more loci of exclusion is needed.