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In order to explore the effect of extract (SNE) on skin wound healing in mice and its mechanism, hemostasis effect of SNE was measured, the mouse skin wound model was established by full-thickness excision. The morphological changes of the wound were observed after the treatment with SNE and the healing rate was measured. The changes of wound histology were observed by hematoxylin eosin (HE) staining, Masson staining and transmission electron microscope (TEM). The expression of cell factors and related proteins was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that the SNE possessed hemostatic function. SNE could obviously improve the healing rate of wound in mouse and shorten time of scab removal compared with the none-treatment (NT) group ( 0.05). These results indicated that SNE possessed obvious activity of accelerating wound healing and inhibiting scar formation, and its mechanism was closely related to hemostatic function, regulation of inflammatory factors, collagen deposition, collagen fiber remodeling and intervening TGF-β/Smads signal pathway. Therefore, SNE may have promising clinical applications in skin wound repair and scar inhibition.
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OBJECTIVE： To study the improvement effects of fibrinolytic enzyme from Sipunculus nudus （SNFE） on hemorheology disorder and vascular endothelium injury in naked acute blood stasis model rats. METHODS： SD rats were randomly divided into control group， model group， aspirin group （100 mg/kg） and SNFE high-dose and low-dose groups （2 500， 5 000 U/kg）， with 10 rats in each group. They were given relevant medicine intragastrically once a day， for consecutive 7 d. One hour after the 6th day of administration， except for control group， other groups were given adrenaline hydrochloride 0.8 mg/kg subcutaneously， and then the acute blood stasis model was induced by ice-water bath. Blood was collected from abdominal aorta 2 h after the next day. Blood rheological parameters such as whole blood viscosity （high， medium and low shear rate）， plasma viscosity， hematocrit， erythrocyte aggregation index and erythrocyte deformability index were measured by automatic rheometer. The contents of NO and ET-1 in plasma and their ratio were determined by ELISA， and the damaged degree of vascular endothelium were observed by HE staining. RESULTS： Compared with control group， whole blood viscosity of high， medium and low-shear rate， plasma viscosity， erythrocyte aggregation index and ET-1 content were increased significantly in model group， while erythrocyte deformability index， NO content and NO/ET-1 ratio were decreased significantly， with statistical significance （P＜0.05 or P＜0.01）. Compared with model group， whole blood viscosity of high， medium and low-shear rate， plasma viscosity， hematocrit， erythrocyte aggregation index and ET-1 content were decreased significantly in SNFE high-dose groups. Erythrocyte deformability index， NO content and NO/ET-1 ratio were increased significantly， with statistical significance （P＜0.05 or P＜0.01）. In SNFE low-dose group， erythrocyte deformability index and NO/ET-1 ratio were increased significantly， while ET-1 content was decreased significantly， with statistical significance （P＜0.05 or P＜0.01）. Vascular endothelial staining showed that compared with control group， the structure of aorta layers in model group was loose and disordered， the endothelial defect was incomplete， the vacuoles increased， and the endothelial damage was obvious. The endothelium of rats in each administration group was damaged to varying degrees， but the degree of injury was lighter than in model group. CONCLUSIONS： SNFE can improve hemorheological abnormalities and vascular endothelial injury in rats with acute blood stasis.
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Objective:To establish the quality standard for Sipunculus nudus polysaccharide. Methods:The water content, igni-tion residues and heavy metals in Sipunculus nudus polysaccharide were determined. Authrone-sulfuric acid colorimetry was used to de-termine the polysaccharide content. Results: The water content in the polysaccharide should not exceed 5. 0%, ignition residues should not exceed 1. 0%, and the content of heavy metals should not exceed 20 ppm. The polysaccharide content should exceed 80%( glucose) . Conclusion:The method is accurate and simple, and can be effectively used in the quality control of polysaccharide in Sipunculus nudus Linnaeu.
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Objective To investigate the protective effects of Sipunculus nudus polysaccharides (SNPS) on rats injured by low-dose irradiation combined with carbon monoxide, benzene and noise. Methods Fifty SD rats were randomly divided into normal control group, model control group, 70mg/(kg·d) SNPS group (SNPS 70 group), 140mg/(kg·d) SNPS group (SNPS 140 group) and 280mg/(kg·d) SNPS group (SNPS 280 group). SNPS was administrated intragastrically once a day before γ irradiation for 7 days. Model control group were given the same volume of 0.9% NaCl. Seven days later, all the rats were sacrificed. Peripheral blood cells were analyzed by auto blood cytometry. DNA in bone marrow cells was determined by ultraviolet spectrophotometry. The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in serum were detected by the reagent kits. The indexes of main organs (liver, spleen and thymus) were also calculated. Results Compared with model control group, peripheral blood PLT, RBC, HCT and HGB increased significantly (P<0.05 or P<0.01), WBC increased a little, SOD activity and DNA in bone marrow increased significantly in SNPS groups, while the content of MDA decreased in SNPS groups compared with that in model control group (P<0.05). No significant change was found of the main organs (liver, spleen and thymus) indexes. Conclusion SNPS may take a protective effect on rats injured by deletion environment factors with increasing WBC and PLT in serum, improving antioxidant activity and promoting the repair of injured bone marrow.
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Objective To investigate the protective effects of Sipunculus nudus polysaccharides (SNPS) on rats injured by low-dose irradiation combined with carbon monoxide, benzene and noise. Methods Fifty SD rats were randomly divided into normal control group, model control group, 70mg/(kg·d) SNPS group (SNPS 70 group), 140mg/(kg·d) SNPS group (SNPS 140 group) and 280mg/(kg·d) SNPS group (SNPS 280 group). SNPS was administrated intragastrically once a day before γ irradiation for 7 days. Model control group were given the same volume of 0.9% NaCl. Seven days later, all the rats were sacrificed. Peripheral blood cells were analyzed by auto blood cytometry. DNA in bone marrow cells was determined by ultraviolet spectrophotometry. The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in serum were detected by the reagent kits. The indexes of main organs (liver, spleen and thymus) were also calculated. Results Compared with model control group, peripheral blood PLT, RBC, HCT and HGB increased significantly (P<0.05 or P<0.01), WBC increased a little, SOD activity and DNA in bone marrow increased significantly in SNPS groups, while the content of MDA decreased in SNPS groups compared with that in model control group (P<0.05). No significant change was found of the main organs (liver, spleen and thymus) indexes. Conclusion SNPS may take a protective effect on rats injured by deletion environment factors with increasing WBC and PLT in serum, improving antioxidant activity and promoting the repair of injured bone marrow.
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Objective To evaluate the protective effects of Sipunculus nudus preparation(SNP) on mice irradiated by 60Co.Methods The mice were divided into control group,model group and 0.5,1.0,and 2.0g?kg-1?d-1 SNP groups.SNP was administrated by intra-gastric infusion with the volume of 0.4mL per 20g.In the fifteenth day,the model group and the treated groups were irradiated by ?-ray of 60Co(0.83Gy/h) with the dose of 5 Gy.Peripheral blood WBC,RBC,PLT,the spleen(SI) and thymus index(TI),the BMNC,SOD and MDA in serum,testicle index,germ cells were detected.Results Comparing with the model group,TI,SI and peripheral blood WBC,RBC and BMNCs were higher.The activities of SOD in serum were increased,while MDA decreased.Testicle index and germ cells were increased,and germ abnomality rate was decreased in SNP groups.Conclusion SNP has protective effects on mice irradiatied by 60Co.