ABSTRACT
Objective To evaluate the inhibitory effect of asiaticoside on bleomycin-induced skin cicatrization. Methods Thirty male C57BL/6 mice were randomly divided into three groups:negative control group,model control group,and asiaticoside group,ten in each group.In model control group and asiaticoside group,1 mg·mL-1bleomycin was subcutaneously injected into the dorsal skin of mice every day;4 h later,1 mL 0.9% sodium chloride solution 1 mL asiaticoside(20 mg·mL-1) was injected into the lesion skin in the model control group and the asiaticoside group,respectively.In the negative control group, the same volume of 0.9% sodium chloride solution was subcutaneously injected into the dorsal skin of the mice at the two time points every day.After 21 days,skin specimens were harvested to observe the histomorphology and detect myofibroblast proliferation and expression of inflammatory factors. Results The skin scar was significantly attenuated in the asiaticoside group as compared with the model control group,and the dermal thickness measured exhibited a gradual decrease in asiaticoside group.The expression of α-antismooth muscle antisbidy and infiltration of inflammatory cells were significantly lower in the asiaticoside group than in the model control group. Conclusion Asiaticoside inhibits the development of skin scar of mice by regulating proliferation and differentiation of myofibroblasts and down-regulating inflammatory cells.
ABSTRACT
Objective: To survey the condition of bacteria on the scar area; to improve the method and outcome of skin with scar prepared for plastic surgery. Methods: 49 patients were divided into group A and B randomly. 24 patients in group A were enrolled in control group and 25 patients in group B were designed for investigation group. Each scar was taken 3 samples to be cultured and submitted for sensitive test on admission day, before and after the skin with scar prepared. Each case was observed on the types and the condition of bacteria distribution on the scar. The outcome of cleaning and disinfection from the area was recorded. The relationship between the bacteria reproduction and types of scar was analyzed, and the observation of the postoperative temperature was made and the duration of antibiotics used and surgical site healing were recorded. Results: The positive rate of bacterial culture was reached 83.33% -88.00%. Two methods of skin prepared can diminish the bacteria reproduction. The outcomes of Group B was better than Group A. The temperature and wound healing days had no significant difference between the two groups. The days used of antibiotics in group B were significantly shorter than group A. Conclusion: The more effective method can improve the clean ingress of the skin than with usual method. 60% bacteria on the area removed was benefit to prevent wound infection and healing.
ABSTRACT
External dacryocystorhinostomy(DCR) has been a main surgery for epiphora since its introduction, offering the advantages of the complete formation of the ostium, the formation of flap, and potential application in all cases suffering from chronic epiphora although the disadvantages of postoperative morbidity and a cutaneous scar. In this study, the authors report the extent of patient's content for epiphora and skin scar in 131 cases treated with external DCR perfomed on 112 patients between december 1994 and January 1996. DCR incision followed the periorbital relaxed skin gension lines in order to minimize cutaneous scar. External DCR were combined with silicone intubation of the lacrimal drainage system. The medical records of all patients undergoing external DCR procedures were reviewed retrospectively in detail. the authors asked about postoperative epiphora and skin sacr through the questionnaire or the telephone. Phtients with follow-up periods less than 6 months or conjunctivo-dacryocystorhinostomy were not included in the study. Of 131 cases, 126 were improved for epiphora in comparision with preoperative state after a single attempt, yielding a success rate of 96.2%. Of 131 cases, 129(98.2%) did not express discontent for skin scar. Although endonasal DCR is considered as an increasingly attractive alternative to external DCR, external DCR is still a good procedure to treat epiphora in unexperienced surgeon for endonasal DCR.