ABSTRACT
Objective:To explore the relationship between the expression of solute carrier family 24 member 5(SLC24A5) and skin melanoma (SKCM) cell proliferation and invasion, and to explore the possibility of SLC24A5 as a potential biomarker and therapeutic target of SKCM.Methods:The bioinformatics method was used to collect relevant information, and then the MEL-526 and SK-HEL-5 melanoma cell models of knockdown SLC24A5 were established. The knockdown efficiency wasverified by Western blot and real-time quantitative polymerase chain reaction (qPCR) experiments, and the cell proliferation and invasion of the SKCM cells after knockdown of SLC24A5 were detected.Results:The expression of SLC24A5 in SKCM tissue was significantly higher than that in normal tissues adjacent to cancer. Patients who highly expressed SLC24A5 had a worse prognosis. After knocking down SLC24A5, the proliferation of MEL-526 and SK-HEL-5 cells was inhibited.Conclusions:SLC24A5 plays an important role in the progress of SKCM and has the potential to become a therapeutic target and biomarker of SKCM.
ABSTRACT
Melanin is a kind of biopolymer, composed of a complex quinine/indole-quinone derived mixture which is produced in melanocytes from tyrosine. More than 100 genes related to melanin deposition have been found in mouse. Slc24a5 (solute carrier family 24, member 5) is a novel gene first cloned in zebrafish. And it has been confirmed that the Slc24a5 is involved in controlling the melanin deposition in zebrafish. Here the full length of the chicken slc24a5 mRNA sequence, its expression profile and a discussion of its relationship to melanin deposition in chicken were reported. Chicken Slc254a5 has a CDS of 1 269bp, predicting a protein of 423 amino acids which is about 80 amino acids shorter than in human and mouse. It has 9 exons and 8 introns and spans more than 1lkb of genome sequence. The quantitative real-time PCR confirmed that the chicken Slc24a5 was highly expressed in the eye of White Leghorn and Beijing Fatty Chickens, and in the eye, skin and muscle of Silky. The relative expression in Silky eye is more than two times that of White Leghorn. The relative expression in Silky skin is about 70 times that of White Leghorn and about 15 times that of Beijing Fatty Chickens. The relative expression in Silky muscle is about 15 times that of White Leghorn and about 3 times that of Beijing Fatty Chickens. And the expression result is in accordance with the melanocyte and melanin deposition in these tissues.