ABSTRACT
OBJECTIVE： To study the bile acid components in snake bile and other animal bile, and establish the quality control method of the snake bile and its preparations. METHODS： Acetonitrile-methanol and 10 mmol•L-1 ammonium acetate was used as mobile phase system and 18 alkyl silane bonded silica gel was used as filling agent. Liquid chromatography-mass spectrometry was used to qualitatively identify and quantitatively analyze the bile acid components in snake bile and other animal bile, and the adulteration control and content determination method of bile acid in snake bile and its preparations were optimized to establish a suitable quality control method of snake bile and its preparations. RESULTS： The examination method was highly specialized and suitable for the quality control of snake bile and its preparations.The linear relationship of sodium taurocholate between 0.302-9.053 μg is good, r=0.999 3, the repeatability test and accuracy test results are good, and the RSD is smaller than 3.0%. CONCLUSION： The established quality control method can guarantee the quality and safety of snake bile and its preparations.