Determination of Content of Antioxidant Sodium Sulfite in Etimicin Sulfate Injection with Ion Chromatography / 中国药学杂志

OBJECTIVE: To establish an ion chromatography method for measuring the content of sodium sulfite as an antioxidant in etimicin sulfate injection. METHODS: DionexIonPac AS11-HC (4 mm×250 mm) and DionexIonPac AG1-HC (4 mm×50 mm) were employed as anion analytical column and anion guard column to separate sodium sulfite and determine its content. Gradient elution was carried out with potassium hydroxide solution at the flow rate of 1.0 mL·min-1. Conductivity detector was used with the suppressor current set at 99 mA, the conductivity pool was maintained at 35℃ and the column temperature was maintained at 30℃. RESULTS: A good linear relationship was found between the peak response and the concentration range of 8-80 μg·mL-1. The detection limit was determined to be 0.02 μg·mL-1 (S/N=3), and the quantitation limit was 0.09 μg·mL-1 (S/N=10). CONCLUSION: In this paper, we set up an analytical technique with such characteristics as rapidity, accuracy and reproducibility, which will serve to control the dosage of sodium sulfite. So far, it seems appropriate to use sodium sulfite as an antioxidant, but its amount in pharmaceutical preparations should be further optimized and decreased in the future.

Improvement of preparing technique for compound glycyrrhiza oral solution / 国际药学研究杂志

Objective To obtain a clear and qualified compound glycyrrhiza oral solution by using NaSO3 and EDTA as stabi-lizers and Tween80 as solubilizer so as to solve the problem of morphine content instability. Methods NaSO34g and EDTA 0.6 g as stabilizers,and Tween803 g as solubilizer were added in the traditional method. The pH of the solution was adjusted to 8.0. Then the solution was obtained and filled in the brown polyester bottle. Results The preparation was clear,qualified and the content of mor-phine was steady. Conclusion The improved method is feasible,simple,stabilized and suitable for manufacturing.

Recuperación y diferenciación de aeromonas con CromoCen AE y CromoCen AGN / Recovery and differentiation of Aeromonas with CromoCen AE and CromoCen AGN

INTRODUCCIÓN: en la actualidad las especies del género Aeromonas han emergido como un problema de salud pública, son ellas los agentes etiológicos de las enfermedades diarreicas con el aumento de la atención médica por años. Los procedimientos convencionales para su diagnóstico son muy engorrosos, laboriosos y duraderos. Una nueva metodología que emplea medios de cultivo cromogénicos ha permitido la simplificación y aceleración de su diagnóstico, que ofrece resultados altamente específicos. OBJETIVO: estudiar el efecto de la combinación de diferentes agentes selectivos de los microorganismos grampositivos sobre el aumento de la capacidad de recuperación, cuantificación y diferenciación de las especies de Aeromonas. MÉTODOS: se estudió el efecto inhibidor de la combinación de agentes selectivos (desoxicolato de sodio (0,05-0,2 g·L-1), sales biliares (0,65 g·L-1), verde brillante (0,025-0,03 g·L-1), cristal violeta (0,001-0,01 g·L-1) y sulfito de sodio (0,8 g·L-1) sobre los microorganismos grampositivos, así como la capacidad de recuperación, cuantificación y diferenciación de las especies de Aeromonas. Como base se utilizó la formulación de CromoCen AGN, sin el desoxicolato de sodio. RESULTADOS: los valores de las productividades de los medios CromoCen AE y CromoCen AGN a partir del inóculo 1,5 × 102 UFC·mL-1 resultaron, para: A. hydrophila 116,8 % y 23,9 %, A. caviae 100,8 % y 3,95 %, A. bestiarium 93,6 % y 28,8 %, A. culicicola 85,1 % y 66,12 %, A. veronii 116,7 % y 59,2 %, A. popoffi 86,56 % y 13,2 %, A. trota 94,8 % y 11,25 % y para A. eucrinophila 103,9 % y 2,80 %. La nueva composición cromogénica logró la diferenciación de los microorganismos por sus características culturales: color, forma, superficie, bordes en las colonias y proteólisis del medio circundante. CONCLUSIONES: la combinación de diferentes agentes selectivos para la inhibición de los microorganismos grampositivos coadyuvo el aumento de la capacidad de recuperación, cuantificación y diferenciación de las especies de Aeromonas.

INTRODUCTION: Species of the genus Aeromonas are a current public health problem, for they are the etiological agents responsible for the growing incidence of diarrheal diseases requiring medical care. Conventional procedures for their diagnosis are very complicated, laborious and time-consuming. A new methodology based on the use of chromogenic culture media allows diagnostic simplification and acceleration, yielding highly specific results. OBJECTIVE: Study the effect of combining several selective agents for gram-positive microorganisms upon an increased capacity for recovery, quantification and differentiation of Aeromonas species. METHODS: Assessment was conducted of the inhibiting effect of combined selective agents (sodium deoxycholate (0.05-0.2 g·L-1), bile salts (0.65 g·L-1), brilliant green (0.025-0.03 g·L-1), crystal violet (0.001-0.01 g·L-1) and sodium sulfite (0.8 g·L-1)) on gram-positive microorganisms, as well as their capacity for recovery, quantification and differentiation of Aeromonas species. The base used was the CromoGen AGN formulation without sodium deoxycholate. RESULTS: Productivity values for the media CromoCen AE and CromoCen AGN based on inoculation of 1.5 × 102 CFU·mL-1 were 116.8 % and 23.9 % for A. hydrophila, 100.8 % and 3.95 % for A. caviae, 93.6 % and 28.8 % for A. bestiarium, 85.1 % and 66.12 % for A. culicicola, 116.7 % and 59.2 % for A. veronii, 86.56 % and 13.2 % for A. popoffi, 94.8 % and 11.25 % for A. trota, and 103.9 % and 2.8 0% for A. eucrinophila. The new chromogenic composition enabled differentiation of microorganisms based on their cultural characteristics: color, shape, surface, colony borders and environmental proteolysis. CONCLUSIONS: Combination of various selective agents for the inhibition of grampositive microorganisms led to an increased capacity for recovery, quantification and differentiation of Aeromonas species.

A Case of Allergic Contact Dermatitis Due to Sodium Sulfite Contained in Nizoral(R) Cream / 대한피부과학회지

Sodium sulfite is an antioxidant, a preservative and a reducing agent, widely used in the foods, beverages, drugs, cosmetics, and pharmaceutical industries. Oral ingestion or inhalation of it in asthmatic patients may provoke asthmatic attack, urticaria, angioedema, and anaphylaxis. A 28-year-old male presented with well-demarcated erythematous scaly patch with itching sensation on the left lower leg. He had applied Nizoral(R) cream on the erythematous patch of the left lower leg for 7 days and the skin lesion was aggravated. Patch tests with Nizoral(R) cream 'as is' showed positive reaction and sodium sulfite, the ingredient of Nizoral cream, revealed positive reaction.

Effects of Sodium Sulfite on Expression of Inflammatory Factor Genes in Human Embryo Kidney 293 Cells / 环境与健康杂志

Objective To investigate the expression of inflammtory factor genes change induced by sodium sulfite.Methods MTS assay was used to detect the cell toxicity to human embryonic kidney cell line 293(HEK293) of 0,0.0025,0.01,0.039,0.156,0.625,2.5,10 mmol/L sodium sulfite,morphological changes were observed with inversion microscope and RT-PCR was used to study the expression of mRNA changes of TNF-?,MCP-1 and IL-8.Results Cytotoxicity analysis showed that treatment of cells with 0.625,2.5,10 mmol/L Na2SO3 could significantly decrease the OD value,with the OD value of(0.354 75 ?0.021 24),(0.600 50?0.012 77),(0.784 75?0.009 85) respectively,compared with control group(2.514 5?0.202 265).When treated with ≤0.156 mmol/L Na2SO3,it sould not significantly affect cell viability,with the OD value of(2.473 75?0.069 99)-(2.625 00? 0.120 29).Morphological observation showed that exposure of ≥0.625 mmol/L Na2SO3 could decrease cell numbers significantly and living cells seemed narrower and longer than the usual way with fewer evection.But lower concentrations of Na2SO3(≤0.156 mmol/L) did not change cell numbers and cell morphology.RT-PCR result showed that treatment of 0.039-10 mmol/L Na2SO3 could not induce the expression of TNF-?,MCP-1 and IL-8.Conclusion Na2SO3 can cause significant inhibition and injury in HEK293,but can not up regulate the expression of mRNA of TNF-?,MCP-1 and IL-8,and there is no obvious relation between them.

Removal of Low Concentration Formaldehyde in Indoor Air by Chemisorption / 环境与健康杂志

Objective To remove formaldehyde of the low concentration in the indoor air and purify the indoor air. Methods The concentration of formaldehyde was determined by MBTH spectrophotometry and the removal efficiency of low concentration formaldehyde in the indoor air by using sodium sulfite, sodium bisulfite, ammonium sulfate, ammonium chloride, ammonium molybdate and potassium permanganate was tested. Results As the concentration of formaldehyde was at 1 mg/L, 10 mg/L and 100 mg/L respectively, the removal rate of formaldehyde of sodium sulfite, sodium bisulfite and potassium permanganate was 15.9%, 74.7% and 93.5% respectively. On the acidity condition or alkalescence, potassium permanganate was also effective in removing of the different concentration formaldehyde was 23.8%, 74.7% and 93.5%. Ammonium molybdate and potassium permanganate could remove the formaldehyde by 25.9% and 35.7% when the concentration of formaldehyde was at 10 mg/L and 100 mg/L. Ammonium sulfate or ammonium chloride could not effectively remove the low concentration formaldehyde and the removal rate was under 7.0%. Conclusion On the acidity condition or alkalescence, potassium permanganate is effective in removing of the low concentration formaldehyde in the indoor air.