Biological Toxicity Effects of Soil Pollution Caused by Galvanized Wastewater Based on Vibrio Qinghaiensis sp.-Q67 / 法医学杂志

Objective To establish a rapid diagnosis method for the biological toxicity of soil, accurately and rapidly evaluate the toxicity of contaminated sites and identify the dominant pollutants. Methods Take the soil pollution of a galvanized factory as an example, while the metal concentration level was analyzed and detected, a rapid biological toxicity detection method based on the acute toxicity test of luminescent bacteria （Vibrio qinghaiensis sp.-Q67） was established, and the dominant pollutants were identified by stepwise multiple regression. Results The pollutants came from wastewater and metal plating fragments directly discharged from the manufacturing line of the factory. The concentration of those pollutants was correlated with the acute toxicity of Vibrio qinghaiensis sp.-Q67. The dominant pollutants in the study were zinc （Zn）, aluminum （Al） and copper （Cu）. Conclusion The luminescent bacteria toxicity test method based on Vibrio qinghaiensis sp.-Q67 can conveniently and rapidly assess the degree of toxic damage of polluted soil and identify the dominant pollutants and can be applied to the acute toxicity evaluation of polluted soil.

Microbiological contamination and content of heavy metals in unlined landfill sites of Ulaanbaatar

Background:The aim of the study was to investigate bacterial and heavy metal pollution in four landfill sites of Ulaanbaatar. Surveyed landfills included closed and currently operating unlined landfills. Methods:In order to sample, we divided each landfill sites into three zones including zone 1: cover soil or in the boundary of the landfill, zone 2: from the boundary of the landfill to hygiene zone (25 м2to 500 м2out of cover soil), zone 3: from hygiene zone to settlement areas and compared the pollution.Results:The titre of Cl.perfringens, presence of Salmonella and total bacteria were not significantly different for landfill sites. The titre of E.coli and the titre of Proteus for industrial landfill sitewere significantly different than other sites.The titre of E.coliand the titre of Proteusfor the zone 1 were significantly different than the zone 3. The presence of Salmonellaand the total bacteria for the zone 1 was significantly different than the zone 2 and zone 3. The titre of Cl.perfringenswas not significantly different for landfill zones. The bacterial indicators were not significantly different for seasons of the year. Content of heavy metals in soil landfills were Pb 0.1-63 mg/kg, Cr 0.4-5124 mg/kg, Ni 3.2-84 mg/kg, Zn 21.7-776 mg/kg, Cd 0.01-2.8 mg/kg.Conclusions:Microbiological contamination and content of heavy metals in closed unlined landfill are not different from currently operating unlined landfills.

Água-régia como extrator alternativo para o diagnóstico ambiental de áreas impactadas por resíduos industriais: estudo de caso Centres (Queimados, RJ) / Aqua regia as an alternative extractor for the environmental diagnosis of areas impacted by industrial waste: case study Centres (Queimados, RJ)

RESUMO Áreas contaminadas por metais podem configurar sério risco à saúde pública e ao meio ambiente. Na década de 1980 foi instalado um pátio de estocagem provisória de resíduos industriais perigosos (CENTRES), no município de Queimados, Rio de Janeiro, o que causou um grande impacto ambiental ao solo da região. A legislação brasileira para o diagnóstico de áreas contaminadas limita a extração de metais em solos aos métodos recomendados pela United States Environmental Protection Agency (USEPA) 3050 e 3051A. Entretanto, muitos pesquisadores utilizam o método com água-régia para a extração de metais em solo, mas são escassos estudos que comparem as concentrações de metais extraídos por água-régia e USEPA 3051A. Logo, o objetivo deste trabalho foi avaliar se o método de extração de metais em solos por água-régia é estatisticamente diferente do método USEPA 3051A. Os resultados obtidos revelaram que as concentrações de Cd, Cr, Cu, Ni, Pb e Zn utilizando ambas as extrações (água-régia e USEPA 3051A) foram estatisticamente semelhantes. Também foi possível identificar que as concentrações de Cu, Ni, Pb e Zn foram superiores ao valor de investigação industrial, o que caracteriza essa área como altamente contaminada e solo classe 4.

ABSTRACT Metal contaminated areas lead to environmental and public health risks. In the 1980s, dangerous industrial waste storage was initiated in the CENTRES area in the municipality of Queimados, Rio de Janeiro, Brazil. The inadequate management of these wastes has promoted a large environmental impact to this region's soils. The Brazilian legislation for diagnostics of contaminated areas limits the extraction of metals on soils to USEPA 3050-B and USEPA 3051-A methods. However, many researchers use the aqua regia method for extracting metals in soil, but there are few studies comparing concentrations of metals extracted by aqua regia and USEPA 3051A. Therefore, the objective of this work was to evaluate if the method of extracting metals in soils by aqua regia is statistically different from US EPA 3051A. The results showed that the Cd, Cr, Cu, Ni, Pb and Zn concentrations using both extractions were statistically similar. It was also observed that Cu, Ni, Pb and Zn concentrations were higher than the industrial investigation value which characterizes this area as highly contaminated and soil class 4.

Variations in cadmium and nitrate co-accumulation among water spinach genotypes and implications for screening safe genotypes for human consumption / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Vegetables are important constituents of the human diet. Heavy metals and nitrate are among the major contaminants of vegetables. Consumption of vegetables and fruits with accumulated heavy metals and nitrate has the potential to damage different body organs leading to unwanted effects. Breeding vegetables with low heavy metal and nitrate contaminants is a cost-effective approach. We investigated 38 water spinach genotypes for low Cd and nitrate co-accumulation. Four genotypes, i.e. JXDY, GZQL, XGDB, and B888, were found to have low co-accumulation of Cd (<0.71 mg/kg dry weight) and nitrate (<3100 mg/kg fresh weight) in the edible parts when grown in soils with moderate contamination of both Cd (1.10 mg/kg) and nitrate (235.2 mg/kg). These genotypes should be appropriate with minimized risk to humans who consume them. The Cd levels in the edible parts of water spinach were positively correlated with the concentration of Pb or Zn, but Cd, Pb, or Zn was negatively correlated with P concentration. These results indicate that these three heavy metals may be absorbed into the plant in similar proportions or in combination, minimizing the influx to aerial parts. Increasing P fertilizer application rates appears to prevent heavy metal and nitrate translocation to shoot tissues and the edible parts of water spinach on co-contaminated soils.

Differential behavior of the summer cover crops in the absorption and translocation of copper / Comportamento diferencial das plantas de cobertura de verão na absorção e translocação do cobre

ABSTRACT: Phytoremediation is an alternative technique used to treat copper-contaminated soils. The objective of this research was to explore the behavior of nine summer cover crops regarding the growth, absorption and translocation of copper in soils with contamination levels exceeding the Value of Prevention, with a view to selecting plants for phytoremediation programs. In the experiments the Cambisol was contaminated with copper, added in doses of 0, 100, 200, 400, 500 and 600mg kg-1, in which the following nine plants were cultivated under greenhouse conditions: Canavalia ensiformis, Cajanus cajan, Dolichos lablad, Mucuna cinereum, Mucuna aterrima, Crotalaria juncea, Crotalaria spectabilis, Pennisetum glaucum and Paspalum notatum. At 90 days after sowing the plants were evaluated for dry mass of shoot and root, Cu, N, P, K, Ca, Zn, and Fe levels in the shoot and Cu in the roots. High soil Cu levels induced a decline in the phosphorus absorption by the plants. Canavalia ensiformis displayed high potential for phytoextraction as these plants could translocate high copper concentrations to the aerial plant parts, while the Mucuna cinereum and M. aterrima are indicated for the copper phytostabilization programs, due to the high copper accumulation in their root systems.

RESUMO: A fitorremediação é uma alternativa para o tratamento de solos contaminados com cobre. O objetivo do trabalho foi conhecer o comportamento de nove plantas de cobertura de verão quanto ao crescimento, absorção e translocação do cobre em solos com contaminação acima dos Valores de Prevenção, com vistas a seleção de plantas para programas de fitorremediação. Um Cambissolo foi contaminado com doses de 0, 100, 200, 400, 500 e 600mg kg-1 de cobre e cultivado em casa de vegetação com nove plantas: Canavalia ensiformis, Cajanus cajan, Dolichos lablad, Mucuna cinereum, Mucuna aterrima, Crotalaria juncea, Crotalaria spectabilis, Pennisetum glaucum e Paspalum notatum. Aos 90 dias após a semeadura as plantas foram avaliadas quanto à produção de massa seca de parte aérea e raízes, teores de Cu, N, P, K, Ca, Zn, e Fe na parte aérea e de Cu nas raízes. As altas concentrações de Cu no solo reduziram a absorção de fósforo pelas plantas. O feijão-de-porco apresenta potencial para a fitoextração, pois apresentou translocação de altas concentrações de cobre para os tecidos aéreos, enquanto que as mucunas preta e cinza são indicadas para programas de fitoestabilização de cobre, pois acumularam elevada concentração de cobre no sistema radicular.

Nickel adsorption and desorption in an acric oxisol as a function of pH, ionic strength and incubation time / Adsorção/dessorção de níquel em solo ácrico em função do pH, da força iônica e tempo de incubação

ABSTRACT Although nickel (Ni) has both important potential benefits and toxic effects in the environment, its behavior in tropical soils has not been well studied. Nickel adsorption-desorption in topsoil and subsoil samples of an acric Oxisol was studied at three pH values (from 3.0 to 8.0). Adsorption-desorption isotherms were elaborated from experiments with increasing Ni concentration (5 to 100 mg L-1), during 0, 4, and 12 weeks, using CaCl2 0.01 and 0.1 M as electrolytic support in order to also verify the effect of Ni-soil time contact and of ionic strength on the reaction. Experimental results of Ni adsorption fitted Langmuir model, which indicated that maximum Ni adsorption (71,440 mg kg-1) occurred at subsoil, after 12 weeks. Nickel affinity (KL) was also greater at subsoil (1.0 L kg-1). The Ni adsorption in the topsoil samples was higher, due to its lower point of zero salt effect (PZSE) and higher organic matter content. The increase in soil pH resulted in the increase of Ni adsorption. Nickel desorbed less from soil samples incubated for 4 or 12 weeks, suggesting that Ni interactions with colloidal particles increase over time. The amount of Ni desorbed increased with increasing ionic strength in both the topsoil and subsoil soil samples. Finally, adsorption-desorption hysteresis was clearly observed. Soil pH, ionic strength of soil solution and the Ni-soil contact time should be considered as criteria for selecting the areas for disposal of residues containing Ni or to compose remediation strategies for acric soils contaminated with Ni.

RESUMO Apesar de o níquel (Ni) ser um elemento importante no ambiente, tanto pelo seu potencial benéfico, como tóxico, informações sobre o seu comportamento em solos tropicais são escassas. Reações de adsorção-dessorção de Ni em amostras superficias e subsuperficias de um Latossolo ácrico foram estudadas em três valores de pH do solo, variando de 3,0 a 8,0. Isotermas de adsorção-dessorção foram elaboradas a partir de experimentos com concentrações crescentes de Ni (5 a 100 mg L-1), durante 0, 4 e 12 semanas, usando CaCl2 0.01 e 0.1 M como eletrólito suporte para verificar o efeito do tempo de contato e da força iônica sobre a reação. Resultados experimentais foram adequadamente ajustados pelo modelo de Langmuir, o qual apontou que a máxima adsorção de Ni (71.440 mg kg-1) ocorreu após 12 semanas de contato do Ni com amostras do subsolo. A afinidade do Ni também foi maior pelas amostras de subsolo (1,0 L kg-1). A adsorção de Ni foi maior nas amostras superficiais, devido ao menor ponto de efeito salino nulo e ao maior tero de matéria orgânica. A elevação do pH do solo aumentou a adsorção de Ni. Houve menos dessorção de Ni das amostras de solo incubadas por 4 e 12 semanas, sugerindo que as interações entre o Ni e as partículas do solo aumentam ao longo do tempo. A quantidade de Ni dessorvido aumentou com o aumento da força iônica, independentemente da profundidade de amostragem do solo. Finalmente, a histerese da reação de adsorção-dessorção de Ni foi claramente observada. O pH do solo, a força iônica da solução e o tempo de contato do Ni com o solo devem ser considerados critérios para a seleção de áreas para a disposição de resíduos contendo Ni e para compor estratégias de remediação para solos ácricos contaminados por Ni.

Studies on chemical and bacterial contamination of soil in Ulaanbaatar / Монголын Анагаах Ухаан

The results of studies on heavy metal and bacterial contamination in Ulaanbaatar since 1990 have beensummed up in this abstract. In the recent years, soil pollution has increased due to rising number ofpopulation, especially in ger districts, factories and services in Ulaanbaatar. According to some surveys,pollution indicators of lead, arsenic and bacterial contamination in soil have exceedednationalstandardlevel.

Assessment of heavy metal content in urban agricultural soils from the surrounding of steel-smelter plant using X-ray fluorescenc / Estudio del contenido de metales pesados en suelos urbanos agrícolas adyacentes a una planta de acero mediante fluorescencia de rayos X

Concentrations of Cr, Co, Ni, Cu, Zn and Pb in the topsoils (0-10 cm) from ten farms located in the vicinity of a steel-smelter plant at Cotorro (Havana, Cuba) were measured by X-ray fluorescence analysis. The concentration ranges of Cr, Co, Ni, Cu, Zn and Pb were 54-186, 15-39, 19-137, 50-945, 91-7739 and 21-731 dry weight respectively. The metal mean contents in the farm topsoil samples were compared with metal contents reported for soils from the vicinity of other smelters worldwide. The Metal-to-Iron normalisation and estimation of the integral pollution indexes allowed observing that most metal polluted soils are from those farms, and that their location coincide with the prevalent wind direction in the studied area. The enrichment index values show that metal concentrations in soils from these farms are above the permissible levels for urban agriculture.

Se determinan por fluorescencia de rayos X las concentraciones de Cr, Co, Ni, Cu, Zn y Pb en los suelos superficiales (0-10 cm) de 10 granjas agrícolas, localizadas en la vecindad de la planta de acero del Cotorro (La Habana, Cuba). Los rangos de concentraciones de Cr, Co, Ni, Cu, Zn y Pb fueron de 54-186, 15-39, 19-137, 50-945, 91-7739 y 21-731 de peso seco respectivamente. Los contenidos medios de metales pesados en los suelos superficiales de las granjas se comparan con los niveles de metales pesados reportados en la literatura para suelos adyacentes a plantas de acero. La normalización de los metales al hierro y la estimación del los índices de polución integral, permitió determinar que los suelos contaminados por metales pesados fueron aquellos, cuya ubicación coincide con la dirección predominante de los vientos en la zona estudiada. Los valores delíndice de enriquecimiento mostraron que las concentraciones de metales pesados en los suelos de esas granjas, superan los niveles permisibles para la agricultura urbana.

HEAVY METAL SOIL POLLUTION IN ULAANBAATAR AND ESTIMATES OF HEAVY METALS IN THE HUMAN BODY / Шинэ санаа Шинэ нээлт

Environmental pollution, manufactured cities related to human activities such as soil contaminated by heavy metals pollution is one of the problems of the world’s major cities. Heavy metals are one of the main sources of pollution and the environment through biogeochemical cycles, and stored for a long time in the body of living organisms, poisoning is able to generate a negativeimpact on human health. Ulaanbaatar, 2010, along the main road in 11 point analysis of 22 soil samples from some of the heavy metal pollution in the soil lead levels were within normal limits,but the high concentration of topsoil is defined. A study conducted in 2011, but the average leadconcentration of 47.3 ppm healthy uncontaminated soil that is 3-4 times larger than defined.Heavy metals in the soil pollution, but pollution levels being conducted quarterly study and their sources of research have been identified. Heavy metal contamination of Ulaanbaatar soil andcalculation of the amount of heavy metals enter the body. Specialized inspection agency of Ulaanbaatar cities laboratory analysis conducted, the data used as descriptive research study design, participated in the study. Metropolitan areas in the 80 point balance divided analyzed by standard analysis of soil samples collected in spring and autumn, MNS5850:2008 was assessed by comparison with the standard.The average amount of lead in the soil of Ulaanbaatar 18.09 mg/kg (95%CI 13.7-22.4mg/kg), and cadmium concentration of 1.02 mg/kg (95%CI 0.7-1.3mg/kg), the mercury concentration of0.03 mg/kg (95%CI 0.006-0.05 mg/kg) that “The quality of the soil, and soil pollutants, maximum permissible elements” MNS5850:2008 standards, compared to less than the maximum allowed. Lead in the soil through the ingestion 11.75x10-3 mg/kg/day (95%CI 8.9-14.55x10-3 mg/kg/day) and cadmium 0.66x10-3 mg/kg/day (95%CI 0.45-0.84x10-3 mg/kg/day) of mercury 0.02x10-3 mg/kg/day (95%CI 0.0-0.03x10-3 mg/kg/day), and inhalation of lead 1.06x10-6 mg/m3 (95%CI 0.80-1.32x10-6 mg/m3) and cadmium 0.06x10-6 mg/m3 (95%CI 0.00-0.08x10-6 mg/m3), dermal adsorption lead 2.62x10-6 mg/kg/day (95%CI 1.98-3.24x10-6 mg/kg/day) and cadmium 0.15x10-6 mg/kg/day (95%CI 0.10-0.19x10-6 mg/kg/day) be digestible. Ulaanbaatar soil containing lead, cadmium, mercury, “The quality of the soil, and soil pollutants, maximum permissible elements” MNS5850:2008 compared to less than the maximum permitted levels. Three entry through access to the body of heavy metals in the soil to estimate the amount of mercury and cadmium lead digestive, respiratory and skin is a little more access.

Chromium accumulation potential of Zea mays grown under four different fertilizers.

Chromium (Cr) contamination in soil is a growing concern in sustainable agriculture production and food safety. We performed pot experiment with chromium (30 mg/ soil) to assess the accumulation potential of Zea mays and study the influence of four fertilizers, viz. Farm Yard Manure (FYM), NPK, Panchakavya (PK) and Vermicompost (VC) with respect to Cr accumulation. The oxidative stress and pigment (chlorophyll) levels were also examined. The results showed increased accumulation of chromium in both shoots and roots of Zea mays under FYM and NPK supply, and reduced with PK and VC. While the protein and pigment contents decreased in Cr treated plants, the fertilizers substantiated the loss to overcome the stress. Similarly, accumulation of Cr increased the levels of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) indicating the enhanced damage control activity. However, these levels were relatively low in plants supplemented with fertilizers. Our results confirm that the maize can play an effective role in bioremediation of soils polluted with chromium, particularly in supplementation with fertilizers such as farm yard manure and NPK.

Nutrients behavior from the association pig slurry and chemical fertilizers on soybean crop

This study aimed at evaluating the effects of pig slurry application with mineral fertilizer on ions leaching from the soil in soybean crop. The experiment was carried out in 24 drainage lysimeters under protection. The soybean cultivar CD 202/COODETEC was sown in a soil that received 0, 100, 200 and 300 m³ha-1of pig slurry in one cycle, with or without mineral fertilizer. There were three samplings of soil throughout the trial to determine the pH, N, NO3-, K+, Ca2+, Mg2+, Na+, Cu+2, Zn+2, OM, CEC, EC and SAR six times during the crop cycle. The yield was determined in the plants. In soil, pig slurry increased the concentrations of pH, NO3-, K+, Zn+2, OM and CEC, while mineral fertilizer increased P and Zn+2concentrations. The limits observed for the leachate parameters did not present environmental problems according to the Brazilian legislation, but in the intermediate and long term, there special attention should be given to NO3-, P, Na+, EC and SAR. Soybean yield was higher with mineral fertilizer and increased with pig slurry application.

Evaluation of genotoxic potential of industrial waste contaminated soil extracts of Amritsar, India.

The rapid increase in population together with unplanned disposal of effluents from various industries has resulted in accumulation of various heavy metals like As, Cr, Cu, Hg, Ni, Pb and Zn in soil ecosystem which ultimately causes DNA damage in living systems. Considering this, the present study was designed to evaluate the content of various heavy metals (Cu, Cr, Co, Hg, Mn, Ni, Zn) and genotoxicity/mutagenicity of soil samples collected from the outskirts of two industries viz. zinc coating industry (SI) and copper sulphate manufacturing industry (SII) employing Allium root anaphase aberration assay (AlRAAA) and Ames assay. The physicochemical parameters like bulk density, water holding capacity, moisture content, pH, nitrates, phosphates and potassium were also estimated. It was observed that SI sample contained Ni (6.86 mg g-1), Zn (6.53 mg g-1), Co (5.05 mg g-1) and Cr (4.49 mg g-1), while SII contained Cu (32.86 mg g-1), Ni (9.66 mg g-1), Co (6.85 mg g-1) and Zn (5.41 mg g-1). In AlRAA assay, the percentage of cells with anaphase aberrations ranged from 3.63 to 10.67 and 0.38 to 4.83% for samples SI and SII, respectively. In Ames test, sample SII was found to be lethal to Salmonella tester strains at all concentrations used, while sample SI was found to be mutagenic in TA100 strains of Salmonella typhimurium. Sample SII was found to be strongly acidic with pH 3.46. The present study focuses on the increasing heavy metal pollution in Amritsar city due to industrial discharges over lands and also infers that both bioassays Ames and AlRAAA can serve as first alert indication of pollution.

Phytoremediation potential of indigenous plants from Thai Nguyen province, Vietnam.

This study was focused on determining Arsenic (As), Lead (Pb), Cadmium (Cd) and Zinc (Zn) in 33 indigenous plants and 12 soil in-situ plant samples in Thai Nguyen Province, Vietnam. The results showed that the soils of surveyed mining areas contained 181.2- 6754.3 mg kg-1 As, 235.5-4337.2 mg kg-1 Pb, 0.8- 419 mg kg-1 Cd and 361.8-17565.1 mg kg-1 Zn depending on the characteristics of each mining site. These values are much higher than those typical for normal soil. The heavy metal uptake into shoots and roots of 33 indigenous plant species was also determined. Two species of the plants investigated, Pteris vittata L. and Pityrogramma calomelanos L. were As hyperaccumulators, containing more than 0.1% heavy metals in their shoots. Eleusine indica L., Cynodon dactylon L., Cyperus rotundus L. and Equisetum ramosissimum (Vauch) accumulate very high Pb (0.15-0.65%) and Zn (0.22-1.56%) concentration in their roots. Additional experiments to clarify the potential of six these plants as good candidates for phytoremediation of heavy metal pollution soil are being carried out in our laboratory.

Formas de perdas de cobre e fósforo em água de escoamento superficial e percolação em solo sob aplicações sucessivas de dejeto líquido de suínos / Forms of losses of copper and phosphorus in water of runoff and percolation in soil with successive pig slurry application

As aplicações sucessivas de dejeto líquido de suínos podem aumentar os teores de cobre e fósforo na camada superficial do solo, aumentando as perdas de suas formas por escoamento superficial e percolação. O trabalho teve por objetivo avaliar as perdas e as formas de cobre e de fósforo na solução escoada, na superfície e percolada em solo submetido a aplicações sucessivas de dejeto líquido de suínos, em sistema plantio direto. O experimento utilizado neste estudo foi instalado no ano de 2000, sobre um solo Argissolo Vermelho distrófico arênico. Os tratamentos foram aplicações sucessivas de 0, 40 e 80m³ ha-1 de dejeto líquido de suínos a lanço e na superfície do solo, em sistema plantio direto. Para este trabalho, foram coletadas soluções escoadas e percoladas, durante o ciclo da aveia preta (Avena strigosa), no inverno de 2006, quando o solo apresentava um histórico de 16 aplicações de dejeto. Nas soluções, foi quantificado o sedimento e determinados os teores de Cu, P, cátions e ânions, pH, carbono orgânico dissolvido (COD) e condutividade elétrica (CE). Usando o programa Visual MINTEQ, versão 2.15, se realizou a especiação iônica de Cu e P nas soluções. As maiores perdas de cobre e fósforo do solo ocorrem por escoamento superficial, em áreas submetidas a aplicações sucessivas de dejeto líquido de suínos em sistema plantio direto. O potencial contaminante desses elementos ficou evidenciado pela presença do cobre na forma predominante orgânica (CuCOD) e do fósforo nas formas livres (HPO4(2-) e H2PO4-), na solução escoada.

Successive pig slurry application can increase the copper and phosphorus levels in the soil and the losses of these elements by surface runoff and percolation. The objective of this study is to evaluate the losses and the forms of both copper and phosphorus in the surface runoff and also in/on the percolation solution, on soils with successive pig slurry application under no tillage system. The experiment was installed in 2000, at Typic Hapludalf soil. The treatments consisted of successive applications of 0, 40 and 80m³ ha-1 of pig slurry applied on soil surface in no tillage system. Samples of surface runoff and percolated solutions were collected during the life cycle of oat (Avena strigosa) in the 2006 winter, when the soil had a history of 16 pig slurry applications. The following parameters have been evaluated: Cu, P, cation, anion and pH, dissolved organic carbon and electric conductivity. The Visual MINTEQ program version 2.15 was utilized to estimate the copper and phosphorus ionic speciation. The surface runoff is the most important form of copper and phosphorus loss of the soil. In addition, copper was found in the organic form (CuCOD) and phosphorus in the free forms (HPO4(2-) e H2PO4-) in the surface runoff solution. Thus, these elements could be an important source of water contamination in places where pig farming is widespread.