ABSTRACT
Objective: To describe the epidemiological features, outcomes and prognostic factors in diagnosis of pediatric hemophagocytic lymphohistiocytosis (HLH). Methods: 118 children fulfilling the inclusion criteria for HLH were identified from review of hospital records for period January, 2010 to December, 2019. Result: Median age at diagnosis was 4 years (range13 days-15 years). Presenting features were fever (100%), hepatosplenomegaly (91%), neurological symptoms (23%), bicytopenia (76%), transaminitis (67.3%), increased soluble interleukin-2 receptor) (sIL-2R) (78%) and hemophagocytosis on bone marrow (75%). Median follow-up duration was 13.5 months (3 days to 102 months). Primary HLH was identified in 27 (23%) patients. Etiology of secondary HLH was infections in 53 (45%), rheumatologic illnesses in 21 (18%) and malignancies in 8 (6%) children. Treatment modalities were steroid only (25%), anti-infectious agent (58%), multi-agent chemotherapy (43%) and HSCT (40%); mortality among above treatment groups were 25%, 58%, 43% and 40%, respectively. 15 patients (13%) had relapsed/refractory HLH who were treated with salvage chemotherapy and hematopoietic stem cell transplantation (HSCT). The overall mortality rate was 39%; mortality within 30 days seen in 23%. Estimated overall survival (OS) and event free survival (EFS) at 3 years were 62% and 61%, respectively. Conclusion: Pediatric HLH is an aggressive disease with high mortality. Hyponatremia, hyperbilirubinemia, coagulopathy and increased sIL2 receptor level at diagnosis predicts poor outcome.
ABSTRACT
PURPOSE: The pathogenesis of atopic dermatitis is not clearly defined yet, but the pathogenetic role of Th2 cells has been supposed. CD30 is a membrane-bound glycoprotein that may be expressed on activated T cells with a sustained expression in Th2 cells and can be released as a soluble form(sCD30). This study was done to document the changes of serum sCD30 and it's clinical significance in atopic dermatitis. METHODS: We analyzed serum sCD30, serum soluble IL-2 receptor (sIL-2R), total serum IgE and total eosinophil counts from 18 children with atopic dermatitis(AD), 15 atopic asthmatics without AD (AA), 15 atopic asthmatics with AD(AD+AA), and 14 healthy non atopics(control). We investigated the correlation of serum sCD30 levels with disease severity assessed by clinical scoring(SCORAD index) in the group of AD and AD+AA. RESULTS: The serum levels of sCD30 were significantly higher in the group of AD and AD+AA than the group of AA and control. There were no differences in serum sCD30 levels between the group of AA and control and between the group of AD and AD+AA. The serum sIL-2R levels showed no significant differences among the four groups. There was significant positive correlation between serum sCD30 and serum sIL-2R levels(P<0.05). Both serum sCD30 and serum sIL-2R levels showed no correlation with total serum IgE, total eosinophil counts, and disease severity, respectively. CONCLUSION: Serum sCD30 is elevated only in atopic dermatitis irrespective of presence of asthma. The results suggest that Th2 immune responses may involved the pathogenesis of atopic dermatitis and sCD30 may be the possible marker of atopic dermatitis.
Subject(s)
Child , Humans , Asthma , Dermatitis, Atopic , Eosinophils , Glycoproteins , Immunoglobulin E , Receptors, Interleukin-2 , T-Lymphocytes , Th2 CellsABSTRACT
The survey of the soluble IL-2 receptor (sIL-2R) level in the sera and the membrane IL-2 receptor (mIL-2R) expression on the peripheral blood mononuclear cells (PBMCs) from 47 cases of late schistosomiasis japonica was reported. The measurement for sIL-2R was done with the double antibody sandwich ELISA. Indirect immunflurescence was performed in the measurement for mIL-2R. The levels of sIL-2R in sera from 47 patients with late schistoso-miasis was found to be higher than that in control (P
ABSTRACT
Serum soluble IL- 2 receptor (sIL-2R) level of 34 patients with leukemia were measured by ELISA. The sIL- 2 R levels are 296?99u /ml in normal, 660 ?449u /ml in AML, 1323 ?755 u/m in ALL, 1577 ? 759 u /ml in AMML and 1815 ? 858 u /ml in NHL. 5 of 6 patients with 2000u /ml sIL- 2 R died in one month after detection of sIL-2R. There is no apparent relationship between sII -2 R level and mononuclear cells count in peripheral blood. The results suggest that it is possible to be a new parameter for the observation and prognosis estimation to patients with leukemia by detection of sIL-2R.
ABSTRACT
In this work, a simple and sensitive assay for soluble IL-2 receptor (sIL-2R) was established. The main procedures are as follows: the microplate was coated with anti-Tac monoclonal antibody (McAb); after sIL-2R in samples bound with the McAb, the rabbit anti-IL-2R serum was added;and HRP-goat anti-rabbit IgG was used to display and amplify this binding. The sensitivity of this method is about 100u/ml, which is near that in "Cellfree IL-2 Receptor Test Kit".
ABSTRACT
Two kinds of mouse monoclonal antibodies which recognize distinct epitopes of the IL-2 receptor (a chain) were used to establish a sandwich ELISA for measuring soluble IL-2 receptors. The results indicated that this method is sensitive and specific for detecting soluble IL-2 receptors of cell culture supernatant and serum from both healthy donor and patients with leukemia. Therefore this sandwich ELISA method is useful for basic and clinic immunology research.