ABSTRACT
AIM To study the chemical constituents from Sonchus oleraceus L..METHODS The 50％ ethanol fraction of 70％ ethanol extract from S.oleraceus was isolated and purfieid by macroporous resin,Sephadex LH-20,ODS and RP-HPLC column,then the structures of obtained compounds were identified by physiochemical properties and spectral data.RESULTS Eleven compounds were isolated and identified as isololiolide (1),bluemenol A (2),5-methoxyisolariciresinol (3),lyoniresinol (4),syringaresinol mono-β-D-glucoside (5),robinin (6),loliolide (7),dehydrovomifoliol (8),5-(1 '-hydroxyethyl)-methyl nicotinate (9),2-methoxy-1,4-benzenediol (10),4-methoxyphenol (11).CONCLUSION Compounds 1-9 are isolated from genus Sonchus for the first time.
ABSTRACT
This study investigated in vitro antioxidant activity of Sonchus oleraceus L. by extraction solvent, which were examined by reducing power, hydroxyl radical-scavenging activity(HRSA) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. 70% MeOH extract had the greatest reducing power while EtOH extract had the greatest HRSA. The antioxidant activity of S. oleraceus extracts was concentration dependent and its IC50 values ranged from 47.1 to 210.5 microgram/ml and IC50 of 70% MeOH, boiling water and 70% EtOH extracts were 47.1, 52.7 and 56.5 microgram/ml, respectively. 70% MeOH extract of S. oleraceus contained the greatest amount of both phenolic and flavonoid contents. The extracts tested had greater nitrite scavenging effects at lower pH conditions. The cytotoxic activity showed that EtOH extract had the best activity against the growth of stomach cancer cell. These results suggest that S. oleraceus extract could be used as a potential source of natural antioxidants.