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Objective: To establish the chemical fingerprint of Sophora alopecuroides extracts based on high performance liquid chromatography (HPLC), and determine the LD50 of different extracts of S. alopecuroides to analyze its “spectrum toxicity” relationship. Methods: A series of extracts were prepared by 75% ethanol reflux (ER), water decoction (WD), 75% ethanol ultrasound (EU) and water ultrasound (WU), and their fingerprints were established to determine the acute toxicity LD50 of different extracts. The relationship between chemical composition and acute toxicity LD50 of S. alopecuroides extracts were studied by means of fingerprint similarity evaluation system. Results: The LD50 of ER, WD, EU, and WU extracts were 38.397, 24.994, 18.536, and 19.957 g/kg, respectively. The ocular lesions of mice viscera were mainly manifested in liver and kidney, and the toxicity of ER extracts was the greatest. The 10 common peaks of S. alopecuroides extracts can be divided into two categories; Peaks 4 and 10, oxymatrine and sophocarpidine were negatively correlated with acute toxicity LD50. Conclusion: The spectral toxicity relationship analysis method of S. alopecuroides was constructed. The unidentified peaks 4, 10 and oxymatrine and sophocarpidine were the main chemical components of the toxicity reaction, which laid a good foundation for clinical application and scientific and rational development of S. alopecuroides.
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OBJECTIVE:To determine the content of sophocarpidine in Fuyankang concentrated pill by HPLC.METHO_ DS:The loading agent of chromatographic column was amino bonded silica,the mobile phase was acetonitrile-dehydrated al?cohol-3%phosphoric acid(80∶10∶10),and the detection wavelength was220nm.RESULTS:There was a good linear rela?tionship between sample size and peak area score of sophocarpidine in the range of0.4064?g～2.0320?g,the average recovery rate was99.2%(RSD=1.6%).CONCLUSION:The established method is more accurate than TLC,and it can be used for the quality control of Fuyankang concentrated pill.
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Objective To determine the in vitro effects of the traditional Chinese medicines, berberine, matrine and baicalin, on the proliferation and lipid synthesis of human immortalized sebocyte SZ95, and to investigate their possible mechanisms of action on sebaceous glands at the cellular level. Methods Inverted microscopy was used to observe cell morphology and determine toxic concentrations of the compounds. The MTT method was adopted to examine the effects of different concentrations of berberine, matrine and baicalin on SZ95 cell proliferation after 24 h, 48 h and 72 h of incubation. Lipid contents in the SZ95 cells were labeled with Nile red and analyzed by flow cytometry. Results The toxic concentrations were 1?10-3 mol/L, 1?10-4 mol/L, 1?10-3 mol/L for baicalin, berberine and matrine, respectively. Berberine reduced sebocyte proliferation in a dose- and time-dependent manner, with an inhibitory concentration 50 (IC50) of 2.9?10-5 mol/L and 1.4?10-5 mol/L after 48 h and 72 h of incubation, respectively. When the concentration of matrine was 0.05). Lipogenesis of SZ95 cells showed a 26.9% increase with 1?10-3 mol/L matrine. Conclusions Our results indicate that berberine and baicalin can inhibit proliferation and lipid synthesis of SZ95 sebocytes in vitro, which suggests a possible clinical role in treating acne.
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Objective To evaluate the effects of monomers of aloin, cinnamic acid and sophocarpidine on the acitivity of tyrosinase,so as to provide depigmenting agents in the treatment of hyperpigmentation disorders and cosmetics additives as well. Methods Tyrosinase activity was estimated by measuring the oxidation rate of DL dopa. The inhibitory pattern of each monomers was determined according to their Lineweaver Burk curve as compared to controls. Results Aloin,cinnamic acid and sophocarpidine down regulated the activity of tyrosinase. The inhibitory rates were significantly higher in cinnamic acid group(2 mmol/L, 0.5 mmol/L), aloin group (2 mmol/L)and sophocarpidine group than those in hydroquinone group (0.5 mmol/L)(P