Spatial distribution and habitat use by early fish stages in a dammed river basin, Southern Brazil / Distribución espacial y uso de hábitat de peces en estadios tempranos de una represa del sur de Brasil

Abstract Fish diversity loss is threatened by the construction of dams as they prevent the regular natural dispersal among populations. Thus, conservation of key riverine habitats for fish reproduction may be essential for the recruitment of new native species of fish. The present study aimed to identify key habitats for fish spawning and early development in the Paranapanema River basin, as well as to determine the taxonomic composition, reproductive and life-history strategy, and to report spatial distribution of eggs, larvae and juveniles. The importance of lagoons, tributaries, and sub-tributaries was evaluated in the Paranapanema River basin between October 2012 and March 2013. Eggs and larvae samples were collected at dawn and dusk with conical plankton nets (0.5 mm mesh size), whereas juveniles were captured during the day with seine and sieve (0.5 cm mesh size). A total of 547 eggs, 904 larvae and 1 228 juveniles were captured. We observed that 2 larvae and 288 juveniles of non-migratory species, parental care, and equilibrium life-history strategy, predominated in lagoons and tributaries. On the other hand, 13 larvae and 60 juveniles of short migratory distance, no parental care, and periodic life-history strategy predominated in sub-tributaries. The highest densities of eggs were recorded in tributaries and sub-tributaries (Tukey's test, P = 0.001 and P = 0.03, respectively), and the highest densities of larvae were recorded for lagoons and tributaries (P = 0.005 and P = 0.0001, respectively). Captures of eggs and larvae were higher at night; while the highest catches per unit effort of juveniles were recorded for tributaries and sub-tributaries. Fish species that adopt different life-history strategies can use diverse types of habitats during the early stages. Lagoons, tributaries and sub-tributaries of the Paranapanema River play different roles in the reproductive success of fish fauna in a heavily modified basin. The preservation of spawning and nursery areas trapped between reservoirs is necessary for Neotropical fish species recruitment and survival. Rev. Biol. Trop. 66(2): 605-621. Epub 2018 June 01.

Resumen Los riesgos de pérdida de diversidad de peces aumentan con la construcción de represas, ya que impide la dispersión de individuos entre las poblaciones. Por lo tanto, la conservación del habitat ribereño clave para la reproducción de peces puede ser esencial para el reclutamiento de nuevos individuos de especies nativas. El presente estudio tiene como objetivo identificar hábitats clave para el desove y el desarrollo temprano de peces en la cuenca del río Paranapanema, así como determinar la composición taxonómica, la estrategia reproductiva y la historia de vida, y reportar la distribución espacial de huevos, larvas y juveniles. Se evaluó la importancia de lagunas, afluentes y subafluentes en la cuenca del río Paranapanema entre octubre de 2012 y marzo de 2013. Se recogieron muestras de huevos y larvas al amanecer y al atardecer con redes de plancton cónico (tamaño de malla de 0,5 mm), mientras que los juveniles fueron capturados durante el día con redes de cerco y tamiz (tamaño de malla de 0,5 cm). Se capturaron un total de 547 huevos, 904 larvas y 1 228 juveniles. Como resultado fue observado que 2 larvas y 288 juveniles de especies no migratorias, cuidado parental y estrategia de vida de equilibrio son predominantes en lagunas y afluentes. Por otro lado, 13 larvas y 60 juveniles de espécies migratorias de corta distancia, sin cuidado parental y estrategia de vida periódica predominaron en subafluentes. Se registraron las densidades más altas de huevos para afluentes y subafluentes (Prueba de Tukey, P = 0.001 y P = 0.03, respectivamente), y se registraron las densidades más altas de larvas para las lagunas y afluentes (P = 0.005 y P = 0.0001, respectivamente). Las capturas de huevos y larvas eran más altas por la noche. Las mayores capturas por unidad de esfuerzo de juveniles se registraron para los afluentes y subafluentes. Las especies de peces que adoptan diferentes estrategias de historia de vida pueden utilizar diversos tipos de hábitats durante las primeras etapas. Lagunas, afluentes y subafluentes del río Paranapanema desempeñan diferentes papeles en el éxito reproductivo de la fauna de peces en una cuenca fuertemente modificada. La preservación de áreas de desove y cría atrapadas entre el embalse es necesaria para el reclutamiento de especies de peces neotropicales.

Identification of fish nursery areas in a free tributary of an impoundment region, upper Uruguay River, Brazil

This study aims to determine the importance of different environments of the Ligeiro River (upper Uruguay River, Brazil) in fish reproduction. For this purpose, three environments (sampling sites) were selected: rapids, a pool, and the mouth of the Ligeiro River. Ichthyoplankton, zooplankton, and benthos were sampled six times per month from September, 2006 to March, 2007. Zooplankton and ichthyoplankton samples were collected early in the evening with plankton nets (64 µm and 500 µm, respectively). Benthos samples were also collected early in the evening with a Van Veen dredge. Local abiotic variables (temperature, dissolved oxygen, pH, electrical conductivity, water speed, alkalinity, water hardness, and water transparency) were measured simultaneously with the biotic data sampling and were complemented by regional variables (water flow and precipitation). A total of 43,475 eggs and 2,269 larvae were captured. Of these larvae, 80.1% were in the pre-flexion and larval yolk stages. Digestive tract content showed that the greatest degree of repletion among the larvae in more advanced phases occurred in the pool environment. Water speed was the main characteristic used to differentiate the river's rapids and mouth from the pool. The abundance of zooplankton and benthos was not related to the distribution of densities among the different components of the ichthyoplankton. A greater abundance of eggs and larvae with yolk was found in the rapids and river mouth. Ordination analyses showed a connection between the advanced stage larvae and the pool environment. In conclusion, the rapids and river mouth of the Ligeiro River's are important locations for fish reproduction, particularly in regard to spawning and drifting of the ichthyoplankton's initial stages, whereas the pool represents a nursery place for larval growth.

O presente estudo visa determinar a importância de diferentes ambientes do rio Ligeiro (alto rio Uruguai/Brasil) na reprodução dos peixes. Para isto foram selecionados três ambientes (locais): uma corredeira, um poço e a foz do rio Ligeiro. As coletas de ictioplâncton, zooplâncton e bentos foram realizadas seis vezes por mês, entre os meses de setembro de 2006 a março de 2007. As amostras de zooplâncton e ictioplâncton foram coletadas no início da noite com redes de plâncton com malha 64 µm e 500 µm, respectivamente. A coleta de bentos também aconteceu no início da noite e foi realizada com uma draga de Van Veen. Variáveis abióticas locais (temperatura, oxigênio dissolvido, pH, condutividade elétrica, velocidade da água, alcalinidade, dureza e transparência da água) foram mensuradas simultaneamente as coletas dos dados bióticos e complementadas por variáveis regionais (vazão da água e precipitação). Foi capturado um total de 43.475 ovos e 2.269 larvas; destas, 80,1% se encontravam nos estágios larval vitelino e pré-flexão. O conteúdo do trato digestório das larvas mostrou que o maior grau de repleção das larvas em estágios mais avançados foi encontrado no poço. A velocidade da água foi o principal fator que discriminou a corredeira e foz do poço. A abundância do zooplâncton e dos bentos não tiveram relação com distribuição das densidades dos diferentes componentes do ictioplâncton. Houve diferença na distribuição dos diferentes componentes do ictioplâncton nos ambientes estudados, com maior abundância de ovos e larvas com vitelo na corredeira e na foz do rio. As ordenações mostraram relação das larvas em estágios avançados com o poço. Conclui-se, portanto, que para a reprodução dos peixes, a corredeira e a foz se apresentam como importantes sítios para a desova dos peixes e deriva dos estágios iniciais do ictioplâncton, enquanto que o poço mostrou relevância como sítio de crescimento das larvas de peixes no rio Ligeiro.