ABSTRACT
Objective:To explore and optimize the preparation method of specimen about mouse liver tissue for scanning electron microscopy.Methods: The specimens of mouse liver using for scanning electron microscope were prepared through improving the fixed location of perfusion, optimizing material and procedure of perfusion, adopting fixation of perfusion via portal vein and changing the process of fixation and so on. And the results of morphological observation of histocyte were compared between the improved method and traditional method.Results: The improved preparation method could save the liver tissue and the living state of the cells in preferably situation. By using this method, the ultra-micro morphological structure of liver tissue and cell could be clearly observed and the ideal effect of observation could be achieved. Besides, its operation was simple and its success rate was high. Therefore, it was superior to traditional method.Conclusion: The preparation of specimen about the fixation of mouse liver tissue via perfusion of portal vein for scanning electron microscope is a superior method, which has certain applied value.
ABSTRACT
Objective: To improve the staining efficiency and quality of biological specimens ultra-thin section for transmission electron microscopy. Methods: The "plug-in staining method" and continuous staining were developed by modifying the traditional staining method. Results: Using our method, we completed the electron staining of a large number of ultra-thin sections of biological specimens of within a short period of time. Conclusion: Compared with traditional method, the new method can save time and drug, and reduce the possibility of staining contamination. The ultrastructure observed by electron microscope is more clear and distinct.