ABSTRACT
An investigation was designed for the phytochemical screening by spectroscopic techniques and to determine the anti-in?ammatory activity of Plumeria rubra L. leaves extract. The qualitative UV-VIS spectrum showed the peaks at 333.15 with absorption 0.0731 respectively. The FT-IR spectrum of the Plumeria rubra L. showed the -1 -1 -1 -1 -1 -1 absorption at 1019.79 /cm-1, 1633.07 cm . 2920.54/ cm , 3416.88/ cm ,481.33/ cm , 700.89/ cm , and 2851.70/ cm , 2926 cm-1, 2855 cm-1, 1609 cm-1. Anti-in?ammatory activity of ethanolic leaf extract of Plumeria rubra L. showed that the percentage of inhibition 54.57% at 500?g/ml concentration was evidently higher than the lower concentration of 50 ?g/ml (8.23%). The presence of various bioactive compounds justi?es the use of the leaves for various ailments by traditional practitioners.
ABSTRACT
Two new sesquiterpenes, trivially named ricinusoids A (1) and ricinusoids B (2), were isolated from ethyl acetate fraction of Ricinus communis. The structures of new compounds were elucidated by detailed spectroscopic techniques, including 1D- and 2D-NMR, UV, IR spectroscopy, and mass spectrometry. The compounds (1-2) were also assessed for in-vivo sedative and analgesic like effects in open field and acetic acid induced writhing tests respectively at 5, 10, and 20 mg·kg i.p. Pretreatment of both test compounds caused significant (P ≤ 0.05) reduction in locomotive activity like sedative agents and abdominal constrictions like analgesics. Both compounds (1-2) possessed marked sedative and antinociceptive effects in animal models.
Subject(s)
Animals , Humans , Male , Mice , Analgesics , Chemistry , Hypnotics and Sedatives , Chemistry , Locomotion , Mice, Inbred BALB C , Molecular Structure , Pain , Drug Therapy , Plant Extracts , Chemistry , Plant Leaves , Chemistry , Ricinus , Chemistry , Sesquiterpenes , ChemistryABSTRACT
Two new sesquiterpenes, trivially named ricinusoids A (1) and ricinusoids B (2), were isolated from ethyl acetate fraction of Ricinus communis. The structures of new compounds were elucidated by detailed spectroscopic techniques, including 1D- and 2D-NMR, UV, IR spectroscopy, and mass spectrometry. The compounds (1-2) were also assessed for in-vivo sedative and analgesic like effects in open field and acetic acid induced writhing tests respectively at 5, 10, and 20 mg·kg i.p. Pretreatment of both test compounds caused significant (P ≤ 0.05) reduction in locomotive activity like sedative agents and abdominal constrictions like analgesics. Both compounds (1-2) possessed marked sedative and antinociceptive effects in animal models.