ABSTRACT
Spent oyster mushroom (Pleurotus florida) compost as tremendous source for isolation of industrial significantenzymes such as amylase, protease, and cellulase. This study was conducted to achieve efficient extraction oflignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), and protease (EC 3.4.21.14) from spent oystermushroom (P. florida) compost waste. Optimal enzyme recovery was achieved when spent oyster mushroom compostwastes and concentrated by acetone precipitation. The purification was performed by column chromatography. Theenzymes such as cellulase, amylase, and protease released from oyster mushroom (P. florida) compost waste wereshowed activities of 15.78 U/ml, 3.42 U/ml, and 0.042 U/ml, respectively. These were utilized in various industrialand environmental applications such as starch processing in potato waste from food industry using amylase andbiotreatment of cotton waste using cellulase.
ABSTRACT
This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at 4degrees C for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.