ABSTRACT
Objective: To investigate the effects of spina date seed (SDS) and albizzia julibrissin flower (AJF) water extracts on the hypothalamic-pituitary-adrenal (HPA) axis and inflammatory factors IF) in the anxiety depression model rats. Methods: A total of 80 Wistar rats were randomly divided into blank group (n= 10), model group (n=10), low dose of SDS group (n=10), high dose of SDS group (n=10), low dose of AJF group (n=10), high dose of AJF group (n= 10), low dose of SDS-AJF group (n= 10), and high dose of SDS-AJF group (n=10). The rat models of anxiety depression were induced by chronic restraint stress, solitary cage and corticosterone (CORT) subcutaneous injection. The forced swimming test (FST) was used to observe the immobility time of forced swimming of the rats in various groups; Elevated plus maze test (EPM) was used to observe the open arm entry (OE), close arm entry (CE), open arm entry time (OT), and close arm entry time (CT) of the rats in various groups. The total entry of open arms and close arms (TE), OE% and OT% were calculated. ELISA method was used to detect the levels of serum corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), CORT, inflammatory factors interleukin-lfi (IL-fi) and interleukin-6 (IL-6) of the rats in various groups. HE staining was used to observe the morphology of hippocampal neurons of the rats in various groups. Results: Compared with model group, the immoblity time of forced swimming of the rats in low dose of SDS-AJF group, high dose of SDS-AJF group, high dose of SDS group and high dose of AJF group was decreased (P<0. 05 or P<0. 01); compared with low dose of SDS group and low dose of AJF group, the immoblity time of forced swimming of the rats in low dose of SDS-AJF group was shortened (P<0. 05 or P< 0.01); compared with high dose of SDS group and high dose of AJF group, the immobility time of forced swimming of the rats in high dose of SDS-AJF group was shortened (P<0. 05 or P<0. 01). Compared with model group, the OE% and OT% of the rats in low dose of SDS-AJF group, high dose of SDS-AJF group, high dose of SDS group and high dose of AJF group were increased (P<0. 05 or P<0. 01); compared with low dose of SDS group and low dose of AJF group, the OE% and OT% of the rats in low dose of SDS-AJF group were increased (P<0. 05 or P<0. 01); compared with high dose of SDS group and high dose of AJF group, the OE% and OT% of the rats in high dose of SDS-AJF group were increased (P<0. 05 or P<0. 01). Compared with model group, the levels of serum CRH, ACTH, CORT, IL-1β, and IL-6 of the rats in low dose of SDS-AJF group, high dose of SDS-AJF group, high dose of SDS group and high dose of AJF group were decreased (P<0. 05 or P<0. 01); compared with low dose of SDS group and low dose of AJF group, the levels of serum CRH, ACTH, CORT, IL-1β and IL-6 of the rats in low dose of SDS-AJF group was decreased (P<0. 05 or P<0. 01); compared with high dose of SDS group and high dose of AJF group, the levels of serum CRH, ACTH, CORT, IL-1β, and IL-6 of the rats in high dose of SDS-AJF group was decreased (P<0. 05 or P<0. 01). The HE staining results showed that the hippocampal neurons of the rats in model group were loosely arranged, and a large number of nerve cells were degenerated and the cell layer was reduced. The degeneration of hippocampal neurons of the rats in low and high doses of SDS-AJF groups were significantly relieved, and the nerve cells were arranged closely and the cell layer was clear. Conclusion: Combination of SDS and AJF can improve the behavior of the rats with anxiety depression, protect the hippocampal neurons of rats, reduce the inflammatory response, and adjust the disorder of HPA axis.
ABSTRACT
Objective:To develop a method of quantitative analysis of multi-components by single marker ( QAMS) for 4 kinds of saponins in spina date seed.Methods:Using jujuboside A as a reference, an HPLC method with a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was applied, the mobile phase was acetonitrile-0.1% phosphoric acid (30 ∶70), the flow rate was 1.0 ml · min-1 , the detection wavelength was 280 nm, the column temperature was 32℃and the injection volume was 20μl.The relative cor-rection factors among the four kinds of saponins were detected by QAMS .An external standard method was also used to determine the contents of the four saponins ( Wf ) , and the results were compared with those of QAMS ( Ws ) , and then the relative errors were calcu-lated between W f and Ws to evaluate the accuracy of QAMS , and the reproducibility was investigated as well .Results:The established QAMS was used to determine the four kinds of saponins in spina date seed , and totally 14 batches of spina date seed were determined . No significant differences were found out between the calculated values and the determined ones ( RSD<5%) .Conclusion:The vali-dated HPLC method shows the advantages of precision ,reproducibility and reliability .The established QAMS method is feasible , and suitable for the quality control of saponins in spina date seed .
ABSTRACT
[Objective] To discuss the mechanism of Spina Date Seed Decoction anti-depression.[Method] Take forced swimming and hanging tail to make desperate rats model;take solidity foster and chronic unpredictable mild stimulation to make mice model.Observe mice immobile time;by weight change and spot box test to appraise Spina Date Seed Decoction influence on chronic stress depressed rats behavior change.Take HPLC to measure rats content of monamine neurotransmitters in the brain;compare groups difference with statistics.[Result] The decoction can markedly improve rats mental sportive depression such as interest loss and lowered movement,as well as increasing the content of monamine neurotransmitters.[Conclusion] Spina Date Seed Decoction has anti-depression function,which is related with its increasing 5-HT and NE in the brain.