ABSTRACT
Many carcinomas have an active mononuclear cell infiltrates surrounding tumor. Various in vitro assays have shown that cellular constituents of peripheral blood mononuclear cells(PBMC) can alter growth of carcinoma cell line. Author compared the effects of normal fibroblasts on squamous cell carcinoma cell line(SCL-1) along with those of sctivated and/or nonactivated PBMC on SCI 1 using a skin equivalent system. This system prevents direct cellular contact by growing SCL-1 on an overlying Millicell-HA membrane and normal fibroblast or supernatants of PBMC in a lower chamber. Normal fibroblasts enhanced the outgrowth of SCL-1 and induced a more organized phenotype of SCL-1. Supernatants from nonstimulated PBMC suppressed outgrowth of SCL 1, and concanavalin A stimulated PBMC supernatants alterd rnorphology of cultured SCL-1 from a disorganized phenotype to a more organized phenotype. It is concluded that fibroblasts and PBMC may affect the growth and differentiation of SCL-1 via their mediators(cytokines)