ABSTRACT
Objective: To investigate the vascular protective mechanism of different levels of herbs through detecting activity of HO-1/CO system in arterial tissues of rats with stagnancy of collateral-Qi in deficiency condition. Methods: Healthy male Wistar rats of cleanness level were randomly divided into control group, stagnancy of collateral-Qi in a deficiency condition group (SCQDC group), Renshen group, Shuangshen group, and Tongxinluo group. The ultrastructure of endothelium cells in the artery was observed. RT-PCR, Western blot, and immunohistochemical approaches were used to detect JNK, c-Jun mRNA and albumen expression in the arterial tissues. Meanwhile, the content of CO in the arterial tissue was also detected. Results: In SCQDC group, the counts of pinocytotic cells and microvili on the free surface of vascular endothelial cells were significantly decreased; most crests of mitochondria fused with membrane, some even disappeared; serious degranulation was observed in the rough surfaced endoplasmic reticulum. Immunohistochemistry showed that the HO-1 positive signals in the arterial histiocyte weakened significantly compared with the control group. The HO-1 expression was increased in the 3 treatment groups. Compared with control group, SCQDC group had decreased HO-1 mRNA and albumen expression(P<0.01,P<0.001). The 3 different kinds of Herbs to dredge collaterals increased the HO-1 mRNA and albumen expression to different degrees (P<0.001). The increase of albumen in the Tongxinluo group was more than those in the Shuangshen group and the Renshen group(P<0.001). Tongxinluo also significantly increased the CO content. Conclusion: Herbs to dredge collaterals can induce CO production by ameliorating or recovering the HO System, which may be one of the mechanisms for protecting the vascular tissues.
ABSTRACT
Objective: To investigate the application of entropy partition method (EPM) for studying the diagnostic criteria of Traditional Chinese Medicine (TCM) syndrome. Methods: A clinical epidemiological survey was conducted in 400 patients with vascular endothelial dysfunction and the EPM was adopted to extract the TCM syndrome elements of "stagnancy of collateral-Qi in a deficiency condition", the correlation degree between each symptom of "stagnancy of collateral-Qi in a deficiency condition" was calculated and the diagnostic threshold of vascular endothelial dysfunction was established. The sensitivity, specificity and accuracy of the EPM-associated diagnostic criteria were assessed by comparing with those of clinical epidemiology. Results: The diagnostic criteria of "stagnancy of collateral-Qi in a deficiency condition" in vascular endothelial dysfunction established with EPM had a satisfactory diagnostic efficiency. Conclusion: EPM for complex system is suitable for studying the diagnostic criteria of TCM syndrome. EPM is a scientific and strict method and has great value in clinical practice.
ABSTRACT
Objective:To investigate the vascular protective mechanism of different levels of herbs through detecting activity of HO-1/CO system in arterial tissues of rats with stagnancy of collateral-Qi in deficiency condition.Methods:Healthy male Wistar rats of cleanness level were randomly divided into control group,stagnancy of collateral-Qi in a deficiency condition group(SCQDC group),Renshen group,Shuangshen group,and Tongxinluo group.The ultrastructure of endothelium cells in the artery was observed.RT-PCR,Western blot,and immunohistochemical approaches were used to detect JNK,c-Jun mRNA and albumen expression in the arterial tissues.Meanwhile,the content of CO in the arterial tissue was also detected.Results:In SCQDC group,the counts of pinocytotic cells and microvili on the free surface of vascular endothelial cells were significantly decreased;most crests of mitochondria fused with membrane,some even disappeared;serious degranulation was observed in the rough surfaced endoplasmic reticulum.Immunohistochemistry showed that the HO-1 positive signals in the arterial histiocyte weakened significantly compared with the control group.The HO-1 expression was increased in the 3 treatment groups.Compared with control group,SCQDC group had decreased HO-1 mRNA and albumen expression(P