ABSTRACT
Objective To explore the risk factors for methicillin-resistant Staphylococcus aureus (MRSA)infection of refractory wound,and provide reference for clinical prevention and control.Methods Clinical data of patients who were isolated Staphylococcus aureus (SA)from wound at the burn ward in a hospital from January 2006 to December 2013 were analyzed,patients were divided into four groups according to whether the isolated SA were MRSA and whether SA were from refractory wound or from non-refractory wound.Risk factors for MRSA infection of refractory wound were analyzed. Results A total of 112 isolates of SA were isolated from wound,statistical differences existed in the length of hospital stay,recent invasive operation,and recent antimicrobial use between patients in refractory wound MRSA group and refrac-tory wound methicillin-sensitive SA (MSSA)group (all P 30 days (OR 95% CI :1.14-30.69)and recent invasive operation (OR 95% CI :1.41 -17.84) were independent risk factors for refractory wound MRSA infection.There were statistically differences in previous MRSA infection,burn depth,recent operation and recent antimicrobial use between refractory wound MRSA group and non-refrac-tory wound MRSA group(all P <0.05);non-conditional logistic regression analysis revealed that recent antimicrobial use (OR 95% CI :2.080-26.800)was independent risk factor for the persistence of MRSA infection of refractory wound. Conclusion Shortening the length of hospital stay,reducing invasive operation,and using antimicrobial agents ra-tionally are helpful for the prevention and control of MRSA infection of refractory wound.
ABSTRACT
Los abscesos pulmonares múltiples se definen como procesos circunscritos, supurativos, bilaterales y menores de dos centímetros de diámetros en el parénquima pulmonar causados por microorganismos piógenos; de los que resultan varias cavidades necróticas. Se presentó un paciente en el cual este proceso se mostró de forma inhabitual. De 48 años de edad, procedencia urbana y ocupación policía con antecedente de diabetes mellitus tipo dos, ingresó por un ántrax en región glútea, que fue tratado quirúrgicamente y con antimicrobianos de amplio espectro que luego de 17 días comenzó con fiebre, tos seca y dolor torácico. Se realizó diagnóstico clínico, imagenológico y microbiológico de abscesos pulmonares múltiples por Staphylococcus aureus.
Multiple lung abscesses are defined as a circumscribed, suppurative, bilateral process. They are smaller than 2cm of diameter in the lung parenchyma caused by purulent microorganism; several necrotic cavities were observed. The objective of this work was to report a case where the process was exposed in an atypical presentation. The evolution of a 48- year- old patient, of urban origin with diabetes mellitus history who was admitted due to anthrax in the gluteus region was presented. Surgical treatment and antimicrobial of wide spectrum were given. After seventeen days of treatment, he started presenting some symptoms such as: fever, cough and thoracic pain. A clinical, radiological and microbiological diagnosis of multiple lung abscesses caused by Staphylococcus aurous was made.
ABSTRACT
Objective To achieve the fusion expression of the entire human beta-defensin-3(hBD-3) gene. Method We synthesized two oligonucleotide primers accor ding to the codon preference of Escherichia coli. The gene was cloned into p GEX -4T-2 to establish the pGEX-4T-2-hBD-3 as the fusion expression vector by PCR. Transformed into E.coli strain DH5α, the express vector was induced an d ex pressed by IPTG. The fusion protein GST-hBD-3 was obtained by repeated cycles of freezing and thawing, cut by thrombin to attain the recombinant hBD-3 protei n. Result The result of the antibacterial peptide agarose diffu sion assay shows the antibacterial activity of the rhBD-3 against the S.aureu s exists, and it reaches 0.843U. Conclusion The fusion expr ession of the hBD-3 gene is successful.