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Abstract Phenolic compounds are one of the main groups of secondary metabolites in plants and are known for their antioxidant activity. Rosmarinus officinalis L. (rosemary) contains different phenolic compounds including carnosol, carnosic acid and rosmarinic acid. In Cuba, rosemary cultivation is limited because it is difficult to propagate and has a low yield. As a result, it was removed from the Herbal Medicine National Formulary. However, the National Public Health System has a strong interest in rosemary because of its value as a natural antioxidant medicine. Irrigation with water treated with a static magnetic field (SMF) is a possible strategy to increase rosemary yield. This technology has been applied to accelerate plant growth and increase crop quality. The aim of this study was to evaluate the content of phenolic compounds and antioxidant activity in aqueous leaf extracts from plants irrigated with SMF-treated water in comparison to control plants. Significant differences in phenolic content and antioxidant activity were observed between aqueous extracts of control plants and plants irrigated with SMF-treated water. Therefore, irrigation with SMF-treated water is a promising technology to improve the cultivation of rosemary as a raw material to obtain pharmaceutical products with high antioxidant activities.
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Rosmarinus/chemistry , Agricultural Irrigation/methods , Magnetic Fields , Antioxidants/analysis , Chromatography, High Pressure LiquidABSTRACT
Chemotherapy is one of the traditional tumors treatment solutions.Chemotherapy has the feature of tissue non-specificity,which can cause side effects on normal cells while inhibiting tumor cell growth.Magnetic targeting drug delivery system (MTDDS) employs biocompatible and stable magnetic nanoparticles (MNP) as drug carries to transport and accumulate anticancer drugs to the specific tumor tissues under the guidance of external magnetic field.This technology not only improves the efficiency of drug delivery and antitumor activity,but also reduces the drug dosage and side effects.The properties of drug-loaded MNPs and the applied external magnetic field are the main factors that affecting the MNPs targeting to the tumor tissues.The effectiveness of the targeted delivery of the drug-loaded magnetic nanoparticles mainly depends on the form and strength of the magnetic field at the target site.That is,whether there is sufficient strength to attract and retain NMPs,and to promote antitumor drug release at the tumor region.In this paper,the research progress of static magnetic field targeting drug delivery system in tumor diagnosis and therapy was summarized,which can provide some basic information for the relative scientific researches.
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Objective To investigate the effect of moderate static magnetic fields (SMF) on secretion of inflammato?ry factors tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) in human monocytic leukemic cell line THP-1. Methods THP-1 cells at logarithmic phase were divided into control group and magnetic treatment group. CCK-8 method was used to detect cell proliferation after THP-1 cells were exposed to 60 mT, 200 mT and 400 mT static magnetic fields at 18, 24 and 48 h. Then THP-1 cells were divided into control group, magnetic treatment group, LPS activation group and LPS+SMF treatment group. When magnetic treatment group and LPS+SMF treatment group were ex?posed to SMF at 18, 24 and 48 h, the levels of the cytokines TNF-α, IL-6 and IL-8 were determined by ELISA. Results (1) 60 mT, 200 mT and 400 mT SMF had no significant effects on cell proliferation in THP-1 cells (P>0.05). (2)THP-1 cells secreted more TNF-αand IL-6 in 24 h than 18 h in every group, while IL-8 didn′t change. Compared with 24 h, the secre?tion of TNF-αdecreased and IL-6 didn′t change, while IL-8 increased in 48 h. At three sampled time THP-1 cells of LPS activation group secreted more TNF-α, IL-6, IL-8 than those of control group and magnetic treatment group. After magnetic treatment THP-1 cells of LPS+SMF treatment group secreted less TNF-α, IL-6, IL-8 than those of LPS activation group (P<0.05). Conclusion Static magnetic field may have some inhibitory effects on release of TNF-α, IL-6, IL-8 from THP-1 cells, which can provide basic data for the treatment of rheumatoid arthritis.
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@#Objective To investigate the effect of exposure to 0.25 T, 0.35 T, 0.42 T static magnetic fields (SMF) on the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs). Methods Primary bone marrow MSCs were obtained from Sprague-Dawley rats and screened by adhesive method. MSCs were exposed to 0.25 T, 0.35 T, 0.42 T SMF continuously and 24 h intermittently respectively.The cell proliferation activity was detected by Cell Counting Kit (CCK-8) assay. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity and osteocalcin were analyzed after continuously exposure to 0.35 T SMF. Results Compared with the control group, the proliferation activity of SMF-treated cells significantly decreased, especially on the 7th day (P<0.001) after continuous exposure,and on the 2nd to 8th day in 0.25 T, 0.35 T SMF intermittent exposure groups (P<0.001). Both the alkaline phosphatase activity and the level of osteocalcin significantly increased in MSCs after continuous exposure to 0.35 T SMF (P<0.05). Conclusion Continuous or intermittent exposure to 0.25 T, 0.35 T and 0.42 T SMF could effectively inhibit the proliferation of MSCs. Continuous exposure to 0.35 T SMF could enhance the osteogenic differentiation of MSCs.
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The effect of magnetic field (MF) treatments of maize (Zea mays L.) var. Ganga Safed 2 seeds on the growth, leaf water status, photosynthesis and antioxidant enzyme system under soil water stress was investigated under greenhouse conditions. The seeds were exposed to static MFs of 100 and 200 mT for 2 and 1 h, respectively. The treated seeds were sown in sand beds for seven days and transplanted in pots that were maintained at -0.03, -0.2 and -0.4 MPa soil water potentials under greenhouse conditions. MF exposure of seeds significantly enhanced all growth parameters, compared to the control seedlings. The significant increase in root parameters in seedlings from magnetically-exposed seeds resulted in maintenance of better leaf water status in terms of increase in leaf water potential, turgor potential and relative water content. Photosynthesis, stomatal conductance and chlorophyll content increased in plants from treated seeds, compared to control under irrigated and mild stress condition. Leaves from plants of magnetically-treated seeds showed decreased levels of hydrogen peroxide and antioxidant defense system enzymes (peroxidases, catalase and superoxide dismutase) under moisture stress conditions, when compared with untreated controls. Mild stress of -0.2 MPa induced a stimulating effect on functional root parameters, especially in 200 mT treated seedlings which can be exploited profitably for rain fed conditions. Our results suggested that MF treatment (100 mT for 2 h and 200 for 1 h) of maize seeds enhanced the seedling growth, leaf water status, photosynthesis rate and lowered the antioxidant defense system of seedlings under soil water stress. Thus, pre sowing static magnetic field treatment of seeds can be effectively used for improving growth under water stress.
Subject(s)
Catalase/metabolism , Catalase/radiation effects , Chlorophyll/metabolism , Chlorophyll/radiation effects , Dehydration , Hydrogen Peroxide/metabolism , Hydrogen Peroxide/radiation effects , Magnetic Fields , Peroxidases/metabolism , Peroxidases/radiation effects , Photosynthesis/radiation effects , Plant Leaves/chemistry , Plant Leaves/enzymology , Plant Leaves/radiation effects , Rain , Seedlings/growth & development , Seedlings/radiation effects , Seeds/radiation effects , Soil , Superoxide Dismutase/metabolism , Superoxide Dismutase/radiation effects , Water/metabolism , Zea mays/growth & developmentABSTRACT
With the rise of magnetic therapy, biological effects of magnetic field have gotten more and more attention in recent years. Based on literatures both at home and abroad, this article summarized research status of biological effects of static magnetic field on whole level, tissue-organ level, cellular level and molecular level of living organisms. We found that conclusions and results of researches varied with different purposes and experiment methods, demonstrating that researches on dose-response relationship and safety of static magnetic field were necessary.
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0.05),while the value of the 120 mT group was lower than the control (P
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Objective To observe the value of the corresponding solutions for MRI static magnetic field artifacts after analyzing their generating mechanism.Methods Different solutions was used to eliminate static magnetic field artifacts,such as improving inner and outer background,calibrating software and hardware,editing parameters,instructing patients,etc.in scanning patients or phantom with Siemens Impact 1.0T and GE Signa 1.5T MR by the use of SE or GRE sequences.Results Static magnetic field artifacts were controlled to different extent and the quality of the mass MR images were improved.Conclusion Most static magnetic field artifacts can be eliminated completely or reduced by using corresponding solutions,so more corrective information can be provided to clinic.
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Objective:To observe the proliferation and the free intracellular calcium ion concentration of osteoblast under the effect of static magnetic field and discuss the possible mechanism of the static magnetic field on promoted the proliferative function. Methods: The MTT assay was used to measure the rate of the proliferation on different magnetic field intensity(0, 0.026, 0.044, 0.090 T)and action time(24, 48, 72 h).The fluorescence double wavelength spectrophotometer was used to measure the concentration of intracellular calcium ion. The data obtained were analyzed for ANOVA and t-test using SPSS 11.0 software package. Results: Osteoblasts in group 0.044 T appeared significant proliferation within 48 h(P
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Objective:To Study the effect of static magnetic field on the proliferation and differentiation of osteoblasts.Methods:In vitro cultured rat calvarial osteoblasts were seeded into 96-well culture plate and exposed to the different magnetic intensity field for 24,48 and 72 h respectively.MTT assay was applied to study the cell proliferation. ALP was measured by a microplate reader.Results:When the magnetic exposure time extended to 48 h or 72 h, the corresponding MTT value of osteoblasts with magnetic treatment of 40 mT or 62 mT increased obviously(P0.05). ALP activity increased after 24 h exposure to static magnetic field, the effect of static magnetic field intensity of 62 mT and 83 mT showed the strongest promotion effect(P
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more efficient tooth movement. The purpose of this study was to evaluate the effect of different static magnetic fields of ND-Fe-B magnet on MC3T3-E1 cells by measuring the alkaline phosphatase activity and observing the amount of stained alkaline phosphatase. For measuring of alkaline phosphatase activity, MC3T3-E1 cells were seeded in first and third row of 12 well culture plate. And Nd-Fe-B magnets were positioned under the first column of first and third row to apply different static magnetic fields(first column:100mT ; second column:4.6mT ; third column:0.5mT ; forth column:0.0mT) to the cells for 7, 13, 19, and 25 days. For staining of alkaline phosphatase, MC3T3-E1 cells were seeded in 100mm culture plates. And Nd-Fe-B magnets were positioned under the corner of plates to apply different static magnetic fields(magnet side:100mT ; the opposite side:0.5mT) to the cells for 7, 13, 19 and 25 days. The results were as follows : 1. ALP activity was increased until day 19 in biochemical determination as well as in histochemical staining. 2. The application of higher magnetic field(100mT) suppressed ALP activity at day 13, 19, 25. On the contrary, the application of the lower magnetic field(4.6mT, 0.5mT) significantly enhanced the ALP activity. 3. Consistent with enzyme assay, histochemical staining of ALP also demonstrated that higher magnetic field(100mT) suppressed ALP activity, lower one(0.5mT) enhanced.
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Alkaline Phosphatase , Enzyme Assays , Magnetic Fields , Tooth Movement TechniquesABSTRACT
To evaluate the effect of a static magnetic field on the bone producing potential of MC3T3 El cells, the alkaline phosphatase activity was measured after the cells having been cultured under 76.4mT static magnetic field using a SmCos magnets for 5days, 7days, lldays, 15days and 2ldays for each cell culture group. Also, the amount of bone nodule stained with Alizarin red S was observed. The results were as follows. · The alkaline phosphatase activity of the 7, 11, and 15 days group among the experimental groups was decreased as compared with the control groups, and the decrease of alkaline phosphatase activity in the 11 days group was the most evident among them. · Any stained bone nodules of both groups had not been observed until the 11th day. The stained bone nodules in the control groups were found on the 15th day, but not in the experimental groups. The stained bone nodules were observed in both groups on the 21st day, but the control groups have more bone nodules than the experimental groups.
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Alkaline Phosphatase , Cell Culture Techniques , Magnetic FieldsABSTRACT
Optimal force for orthodontic treatment is the force that produces a rapid rate of tooth movement without discomfort to the patient or ensuing tissue damage. Recently considerable interest has been generated in the application of magnets as a way to obtain an optimal force. The purpose of the present study was to investigate the effect of static magnetic fields of Sm-Co magnets on molecular and cellular activities. The distance of erythrocyte sedimentation was measured directly, and the acticities and the ayntheses of Fe2+ -related enzymes (xatalase and NO synthase) and non Fe2+ -related enzyme (lactic dehydrogenase) were assayed by the spectrophotometer. The growth and the proliferation of osteoblast-like cells MC3T3-E1 were determined by the crystal violet staining and the 3H-thymidine incoroiration. The erythrocytes were exposed to the pole face flux density of 1,400 G(gauss), and the enzymes and osteoblast-like cells MC3T3-E1 were exposed to the flux density of 7,000 G. The results obtained were as follows: 1. The distance of sedimentation of erythrocyte was not affected by the static magnetic fields. 2. The activities of catalase and lactic dehydrogenase were not affected by the static magnetic fields. 3. The intracellular syntheses of NO synthase and latic dehydrogenase were not affected by the static magnetic fields. 4. The growth and the proliferation if cultured osteoblast-like cells MC3T3-E1 were not affected by the static magnetic fields. There results suggested that the molecular and cellular activities were not significantly influenced by the static magnetic fields.