ABSTRACT
@#This study aimed to investigate the protective effects of Baibu Tang on bleomycin-induced pulmonary fibrosis in mice. After intratracheally giving bleomycin(3. 5 mg/kg), mice were orally administered Baibu Tang once a day for 14 consecutive days, takingnintedanib as a positive control. The anti-fibrotic effects were assessed by the hydroxyproline level and the histopathological changes in H&E or Masson stained lung tissues. The results revealed that the lung coefficient, hydroxyproline content, inflammation and collagen deposition were increased significantly in the lung tissue of the model mice. Both ethanol and water extracts of Baibu Tang significantly improved all the pathological indexes in mice, but the effect of the ethanol extract was better than that of the water extract. Baibu Tang with Baibu(root of Stemona tuberosa)containing different components(neotuberostemonine, tuberostemonine and stemoninine, respectively)could significantly reduce hydroxyproline level and collagen deposition in the lung tissue of bleomycin-induced mice, and there was no significant difference in their activity. This result showed that the changes in the chemical composition of Stemona tuberosa(Baibu, monarch drug for Baibu Tang)have little effect on the anti-fibrosis activity of Baibu Tang, and its mechanism and material basis need further investigation.
ABSTRACT
OBJECTIVE:To study the relieving cough and eliminating phlegm effects of stemoninine combined with mogrosideⅤ on mice,and select its optimal ratio. METHODS:70 mice were selected in each experiment and randomly divided into 7 groups,namely solvent group(normal saline), codeine(15 mg/kg)or ambroxol(30 mg/kg)group(positive control),stemoni-nine group(30 mg/kg),mogroside Ⅴ group(30 mg/kg),stemoninine-mogroside Ⅴ combination group(30 mg/kg)with mass ra-tio of 2:1,1:1,1:2,ig,once a day. Ammonia-induced cough experiment(positive drug was codeine)and tracheal phenol red ex-cretion experiment(positive drug was ambroxol)were respectively conducted. Using cough latent period,cough times and volume of tracheal phenol red excretion as indexes,the antitussive and expectorant effects of drug in each group were compared. RE-SULTS:Compared with solvent group,cough latent period was obviously shortened,cough times was obviously reduced,volume of tracheal phenol red excretion was obviously increased in each administration group(P<0.05 or P<0.01). Compared with mogro-side Ⅴ group,cough latent period was obviously shortened,cough times was obviously reduced in stemoninine-mogroside Ⅴgroup with different mass ratios(P<0.05),and 2:1,1:1 groups showed the best effects. Compared with stemoninine group,vol-ume of tracheal phenol red excretion was obviously increased in stemoninine-mogroside Ⅴ group with different mass ratios (P<0.05),and 1:1,1:2 groups showed the best effects. CONCLUSIONS:The combination of temoninine and mogroside Ⅴ shows synergistic effects on relieving cough and eliminating phlegm,stemoninine-mogrosideⅤmass ratio of 2:1,1:1 can be used as pre-ferred combination of reference.
ABSTRACT
OBJECTIVE:To prepare Stemoninine orally disintegrating tablets,and to optimize its formulation and preparation technology. METHODS:Direct powder compression method was used to prepare Stemoninine orally disintegrating tablets. Using material angle of repose,disintegration time and taste evaluation as index,single factor test was used to screen several factors as bulking agent,disintegrating agent,glidant and flavoring agent;using disintegration time as index,L9(34) orthogonal test was used to optimize the formulation with ratio of MCC+mannitol,PVPP,silica powder and aspartame+stevia as factors. Validation test was also conducted. RESULTS:Optimized formulation was that MCC+mannitol(1∶1)was 50%,PVPP was 20%,silica powder was 2% and aspartame+stevia(10∶1)was 7%. 3 batches of prepared Stemoninine orally disintegrating tablets were smooth in sur-face and good in taste;their disintegrating time was(22.6±2.1)s,and weight variation,hardness,contents were within the speci-fied range (all RSD≤0.97%,n=3). CONCLUSIONS:The formulation and technology of Stemoninine orally disintegrating tab-lets are reasonable,and the quality indexes are all in line with the requirements of orally disintegrating tablets.