ABSTRACT
OBJECTIVE:To establish HPLC fmgerprints of Stephania kwangsiensis.METHODS:HPLC method was adopted.The determination was performed on Hypersil ODS2 with mobile phase consisted of acetonitrile-0.5％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 282 nm,and column temperature was 30 ℃.The sample size was 10 μL.Using tetrahydropalmatine as reference,HPLC fingerprints of 10 batches of medicinal materials were determined.Common peak identification and similarity evaluation were conducted by using Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition).RESULTS:A total of 17 common peaks were identified in HPLC fingerprints of 10 batches of S.kwangsiensis.Among 10 batches of samples,fingerprint of samples from 8 producing areas were compared with control chromatogram.The similarity was higher than 0.900.The similaritg of samples from 2 producing areas were lower than 0.900.CONCLUSIONS:Established HPLC fingerprint can provide reference for the identification and quality evaluation of S.kwangsiensis;S.kwangsiensis in Guangxi from most producing areas include similar alkaloid components,but samples from other producing areas are different from them.
ABSTRACT
OBJECTIVE:To establish HPLC fmgerprints of Stephania kwangsiensis.METHODS:HPLC method was adopted.The determination was performed on Hypersil ODS2 with mobile phase consisted of acetonitrile-0.5％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 282 nm,and column temperature was 30 ℃.The sample size was 10 μL.Using tetrahydropalmatine as reference,HPLC fingerprints of 10 batches of medicinal materials were determined.Common peak identification and similarity evaluation were conducted by using Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition).RESULTS:A total of 17 common peaks were identified in HPLC fingerprints of 10 batches of S.kwangsiensis.Among 10 batches of samples,fingerprint of samples from 8 producing areas were compared with control chromatogram.The similarity was higher than 0.900.The similaritg of samples from 2 producing areas were lower than 0.900.CONCLUSIONS:Established HPLC fingerprint can provide reference for the identification and quality evaluation of S.kwangsiensis;S.kwangsiensis in Guangxi from most producing areas include similar alkaloid components,but samples from other producing areas are different from them.