ABSTRACT
Introducción: La infección de la herida quirúrgica en apendicitis aguda complicada es frecuente. Objetivo: El objetivo fue comparar la solución Dakin y la Superoxidativa para prevenir infecciones de herida quirúrgica en pacientes con apendicitis aguda complicada. Métodos: Estudio comparativo, transversal, en 104 pacientes con apendicitis aguda complicada (Edad media: 36.29 años, 69(66.43%) hombres). Grupo-1: 52 pacientes, con lavado de herida quirúrgica con solución Dakin modificada. Grupo-2: 52 pacientes con solución superoxidativa (Microdacyn®). Se administró ceftriaxona 1 gr antes de la cirugía, se realizó apendicectomía convencional y cierre de pared con Vicryl-1 y Nylon-2/0. Se evaluó herida quirúrgica 7 días después de la operación, registrando presencia de pus, edema, eritema y calor local. Se utilizaron X2 y t de Student. Resultados: Se presentó infección de herida quirúrgica en 11(10.6%) pacientes; 3(5.8%) pacientes del Grupo-1 y 8(15.4%) del Grupo-2 (p=0.1). Conclusión: Ambas soluciones son útiles para prevenir infecciones de herida quirúrgica en pacientes con apendicitis aguda complicada.
Introduction: The infection of the surgical wound in a complicated acute appendicitis is common. Objective: The objective was to compare Dakin and Superoxidative solutions in preventing surgical wound infections in patients with complicated acute appendicitis. Methods: Comparative, cross-sectional study of 104 patients with complicated acute appendicitis (Average age: 36.29 years, 69 (66.43%) men). Group-1: 52 patients, with surgical wound wash using modified Dakin's solution. Group-2: 52 patients with superoxidative solution (Microdacyn®). Ceftriaxone 1 gr was administered before surgery, conventional appendectomy was performed, and the wall was closed with Vicryl-1 and Nylon-2/0. The surgical wound was evaluated 7 days after the operation, noting the presence of pus, edema, erythema, and local heat. Chi-squared (X2) and Student's t-tests were used. Results: Surgical wound infection occurred in 11 (10.6%) patients; 3 (5.8%) patients from Group-1 and 8 (15.4%) from Group-2 (p=0.1). Conclusion: Both solutions are useful in preventing surgical wound infections in patients with complicated acute appendicitis.
ABSTRACT
【Objective】 To screen the sterilizing-grade filters applicable for production of human coagulation factor Ⅷ/von Willebrand factor complex(FⅧ/VWF)and study the sterilization filtration process. 【Methods】 Four sterilizing-grade filters for FⅧ/VWF were evaluated through indicators such as filtration capacity, filtration flux, recovery rate of FⅧ activity, recovery rate of VWF activity, recovery rate of VWF antigen, recovery rate of protein and VWF molecular distribution. The sterilizing-grade filter with the best filtration performance was selected for further study. The study was designed by general full-factor design to determine the appropriate filitered protein concentration and filitered speed range through evaluating the total filtered protein amount, recovery rate of protein and filtration efficiency, and then the process operation parameters was determined. 【Results】 The filtration flux of Sartobran P, Sartopore 2 XLG, Sartopore Platinum and Sartopore 2 XLI were 1.71±0.01, 1.80±0.01, 1.34±0.01, and 1.81±0.04 L·(m2)-1·min-1, respectively; the recovery rates (%) of FⅧ activity were 97.09±2.82, 99.22±0.99, 96.87±1.85 and 93.76±1.21, respectively; the recovery rates (%) of VWF activity were 98.12±1.42, 99.95±1.85, 94.80±1.62 and 92.09±1.67, respectively. Between Sartopore 2 XLG and Sartobran P, the difference of filtration flux (P<0.001) was statistically significant; between Sartopore 2 XLG and Sartopore Platinum, the differences of the filtration flux (P<0.001) and VWF potency recovery rate (P<0.05) were statistically significant; between Sartopore 2 XLG and Sartopore 2 XLI, the differences of FⅧ potency recovery rate (P<0.01) and VWF potency recovery rate (P<0.01) were statistically significant. The optimal process operating space of Sartopore 2 XLG was protein concentration of 0.45-0.58 mg/mL, and filtration rate of 1.48-2.95 L·(m2)-1·min-1. 【Conclusion】 Sartopore 2 XLG is the most suitable filter for the production of FⅧ/VWF and the DoE test proves that it has good process operation space.
ABSTRACT
Currently available antiviral treatment can not eradicate hepatitis B virus completely, and needs long-term or lifelong therapy for the majority of patients with chronic hepatitis B. New antiviral inhibitors and immune modulatory therapies are in development, and have opened new possibilities to cure chronic hepatitis B. This paper summarises status and progress in chronic hepatitis B cure.
ABSTRACT
Objetivo: El objetivo de esta investigación fue valorar la eficacia del activo Nbelyax del esterilizante en frío Éviter™ con presencia de nanotecnología en tres diferentes cepas bacterianas que son de importancia en el sector odontológico: E. faecalis, S. aureus y Neisseria sp. Materiales y métodos: se realizó un estudio experimental, observacional, cualitativo empleando las tres cepas. Se preparó el producto esterilizante Éviter a una dilución de 1:20. En un matraz Erlenmeyer de 250 mL se colocaron 99 mL del producto preparado, donde se inoculó cada cepa bacteriana, se dejó en contacto a dos tiempos diferentes: 5 y 30 minutos. Posteriormente se hizo el vaciado en placa de alícuota de 0,1 mL en 18 mL de soya tripticasa por cada caja de Petri, se incubó 48 horas a 37 °C, y se montó el experimento por duplicado. Para el análisis de resultados se utilizó el programa estadístico SPSS versión 20.0 aplicando la prueba estadística McNemar. Resultados: mostraron que después de 5 minutos de contacto con el esterilizante en frío Éviter, las cepas E. faecalis y S. aureus presentaron desarrollo bacteriano; sin embargo, Neisseria sp. no mostró desarrollo. Las tres cepas bacterianas después de 30 minutos de contacto no presentaron desarrollo colonial. Al aplicarse la prueba estadística McNemar no hubo diferencias estadísticamente significativas (p?#8805;0,05). Con la muestra y metodología usada, se observó que el esterilizante quirúrgico en frío Éviter fue eficaz para eliminar los microorganismos de prueba en 30 minutos.
Purpose: The goal of this research was to evaluate the effectiveness of the active Nbelyax cold sterilant (Éviter™) with nanotechnology in three different bacterial strains of great importance in dentistry: E. faecalis, S. aureus and Neisseria sp. Materials and Method: An experimental, observational and qualitative study using three different bacterial strains was performed. The sterilant product was prepared at 1: 20 dilution. Then, in a 250 mL Erlenmeyer flask were poured 99 mL of the sterilant prepared, each bacterial strain was inoculated in same flask, allowing two different contact times: 5 and 30 minutes. Subsequently, an aliquot of 0.1 mL in 18 mL of trypticase soy per petri plaque was done using pour plate technique and incubated 48 hours at 37 °C. The experiment was done by duplicate. The SPSS statistical program version 20.0 was used, applying Mcnemar statistical test to analyze the results. Results: This research showed that after 5 minutes of contact with the cold sterilant Éviter, E. faecalis and S. aureus strains had bacterial growth, however Neisseria sp. did not have bacterial colonies growth. The three bacterial strains after 30 minutes of contact did not show colonies growth. McNemar test did not show significant statistical differences (p?#8805;0.05). Conclusions: With the sample and ramimethodology used, we observed that the recommended contact time of the cold sterilant Éviter product with nanotechnology to eliminate the microorganism tasted was 30 minutes.
ABSTRACT
En el estudio se desarrollaron métodos de análisis físico-químicos y microbiológicos con la finalidad de evaluar un nuevo producto ADASPOR (ácido peracético estabilizado con adazone), utilizado en el proceso de esterilización como alternativa química aplicado en servicios de microcirugía oftalmológica en el Instituto de Oftalmología Ramón Pando Ferrer. Como metodología de análisis se siguieron técnicas estandarizadas internacionalmente, descritas en la Farmacopea Americana (USP XXIII), normas cubanas, francesas y estadounidenses. Las evaluaciones realizadas demostraron la estabilidad de la solución a la dilución de uso y la compatibilidad con los dispositivos médicos utilizados durante 12 días de trabajo, en un período de análisis se obtuvo reducción total del número inicial de unidades formadoras de esporas/mL, así como la cuantificación del número de ciclos de inmersión (380 c/i) realizados en el período de uso establecido por el fabricante
The aim of present study was to develop methods of physicochemical and microbiologic analyses to assess a new product ADASPOR (adazone-stabilized periacetic acid), used in the sterilization process as a chemical option applied in ophthalmology services in the Ramón Pando Ferrer Institute. As analysis methodology the worldwide standardized standards were followed, described in the American Pharmacopeia (USP XXIII), Cuban, French and American standards. The assessments carried out demonstrated the stability of solution to used dilution and the compatibility with medical devices used for 12 working days in analysis period there was a total reduction of initial number of spores-forming units/mL, as well as the quantification of the number of immersion cycles (380 d/i) carried out during the use period established by manufacturer
ABSTRACT
Due to the growing number of outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is an important and often challenging task. In order to study and compare the behavior of selected microorganisms, they were submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds (QACs); 63 to 10000 mg/L of chlorhexidine; 1375 to 3250 mg/L of glutaraldehyde; 39 to 246 mg/L of formaldehyde; 43750 to 87500 mg/L of ethanol; 1250 to 6250 mg/L of iodine in polyvinyl-pyrolidone complexes, 150 to 4491 mg/L of chlorine-releasing-agents (CRAs) and 469 to 2500 mg/L of hydrogen peroxide. Chlorhexidine showed non inhibitory activity over germinating spores. A. calcoaceticus showed resistance to the majority of the agents tested, followed by E. cloacae and S. marcescens.
Devido ao número crescente de surtos de infecção hospitalar, torna-se proeminente o estabelecimento de um programa de sanitização que liste os agentes químicos a serem empregados e o modo de aplicação mais efetivo. Validação da eficácia de descontaminação é uma tarefa ao mesmo tempo importante e desafiadora. Para estudar e comparar o comportamento dos microrganismos selecionados foram realizados ensaios de concentração inibitória mínima (CIM). A CIM capaz de reduzir o bioburden inicial (>6 log10) foi: 59 - 156 mg/L de quartenários de amônia; 63 - 10000 mg/L de clorexidina, 1375 - 3250 mg/mL de glutaraldeído, 39 - 246 mg/L de formaldeído, 43750 - 87500 mg/L de etanol 1250 - 6250 mg/L de PVPI, 150 - 4491 mg/L de compostos liberadores de cloro e 469 -2500 mg/L de peróxido de hidrogênio.
Subject(s)
Disinfectants/analysis , Diagnosis/analysis , Microbial Sensitivity Tests , Sterilizing Agents , Health Surveillance , Product Surveillance, Postmarketing , Quality ControlABSTRACT
OBJECTIVE To investigate the clinical degree of satisfaction to sterilizing-supplying center,and to consummate the development of the transition of its decentralized administration to centralized pattern.METHODS We designated the questionnaire of degree of satisfaction(DOS) and carried out the investigation.RESULTS The initiated DOS was 90.6%,the DOS in mid-stage(2006-2007) was 97.3%,and the DOS in the latest stage(2008) was 99.1%.CONCLUSIONS Centralized administration is effective pattern for the development of sterilizing-supplying center and important to ensure the quality of sterile materials and service for clinical medicine.
ABSTRACT
OBJECTIVE To discuss rational layout of Sterilizing and Supply Room in the Department of Stomatology and its scientific utilization of sterilizing equipment which ensure safe medical treatment of patients. METHODS Funds should be invested to the Stomatology Department according to plan and environment and equipment should be transformed. RESULTS Transformed conditions of Sterilizing and Supply Room had conformed to Operation Rules of Sterilization of Dental Medical Instruments in Medical Institutions promulgated by the Ministry of Health.One patient could certainly use his own medical goods with absolute sterilization.This not only reduced opportunities of cross infection,but also protected and made things convenient for medical personnel. CONCLUSIONS The fundamental guarantee of safe medical treatment of patients is rational layout of Sterilizing and Supply Room in the Stomatology Department and rational allocation and scientific utilization of sterilizing equipment.
ABSTRACT
OBJECTIVE To study the influence to the sterilizing effect of these factors,which were preheating the sterilizer,the positions of the articles placed and the cleanness of the internal wall.METHODS A total of 120 clinical used dental handpieces,according to the high-pressure steam sterilizer disposal procedure,were divided into four groups,with 30 handpieces in each group.Those only with high-pressure steam sterilizer process were chosen as the control group.Preheating 30 minutes,wipping the internal wall with 0.5% chlorine disinfectant before using high-pressure steam sterilizer and acting both of two steps were all chosen as the experimental groups.Each group was put in the interior,middle and exterior of the high-pressure steam sterilizer separately.The Rapid Readout Biological Indicators(3M Attest,the USA) were used to test the sterilizing effect.RESULTS The different methods of the high-pressure steam sterilizer had the significant differences to the handpieces sterilizing effect(P0.05).CONCLUSIONS To achieve ideal sterilization,it is suggested to wipe the internal wall and the temperature control probe head with 0.5% chlorine disinfectant and preheat the sterilizer for 30 minutes before put handpieces in the high-pressure steam sterilizer.
ABSTRACT
Objective To find out sterilization quality of pulsating vacuum sterilizer for better sterilization effect.Methods The sterilization quality was monitored by physical,chemical and biological methods.Results 4,576 subjects were involved in the test.Such data were obtained as the qualification rate for temperature,the one by chemical indicator card,the one by B-D test and the one by biological indicator,which were 99.6%,99.8%,99.9% and 100% respectively.Conclusion Above data proves that the sterilizer is in normal condition.
ABSTRACT
A new combination process of sterilizing, deplug and waterflood-increasing with sodium hypochlorite in waterflooding horizons has been developed. Its applicability was certified by 4 treatments of injection wells. After treatments the water-intake capacity of injection wells was increased, the pressure at well heat was decreased, and the average duration of stability wster injection was 307 days.
ABSTRACT
Objective To improve the management and utilization of hospital sterilizing devices. Method Strict and operable managerial system was established. Result Hospital sterilizing devices behaved well. Conclusion Just like the purchase, utilization and maintenance of large-scale equipments, the ones of hospital sterilizing devices also should be integrated to optimize their performances.