ABSTRACT
ObjectiveThe aim of this study is to investigate change of platelet count in red blood cell （RBC） units at different storage periods and explore the efficiency of platelet removal by leukocyte filter. MethodsA total of 58 RBC units were divided into four groups according to different storage periods： 1 week Group （16）， 2 weeks Group （16）， 3 weeks Group （14） and 4 weeks Group （12）. RBC units in the four groups were filtered through leukocyte filter. The RBC samples before and after filtration were obtained. The platelet count was detected by automatic blood cell counter and the efficiency of platelet removal was calculated. RBC samples before filtration were made into blood cell smears. The blood cell smears were dyed with Wright-Giemsa stain， and the morphology of platelets was observed through a microscope. ResultsThe platelet count in RBC units stored for 1， 2， 3 and 4 weeks was （286.5±62.34）×109/L， （238.0±57.37）×109/L， （193.6±56.21）×109/L and （167.8±24.76）×109/L， respectively. Platelet count in blood stored for 3 weeks （P<0.01） and 4 weeks （P <0.000 1） were significantly lower than those stored for 1 weeks. When observed in the blood smears of RBC units at different storage periods， platelets with normal morphology were distributed in clump and scattered style. The platelet removal rates of the four groups were （80.13±9.06） %， （76.41±10.13） %， （77.78±9.30） % and （70.63±9.39） %， respectively， with no significant difference （P >0.05）. ConclusionsPlatelet count in RBC units decreases gradually as the storage period increases， but most platelets still remain in RBC units of late storage periods （3 weeks and 4 weeks）. The leukocyte filter is able to remove most of the platelets， and the removal efficiency is similar among the groups.
ABSTRACT
Objective To study the effect of time and temperature on the count of peripheral blood cells in newborns.Methods The peripheral blood of 100 cases of newborns who were admitted in the First Affiliated Hospital of Hebei North University from January 2016 to June 2016 were collected,and measured with Sysmex XS-500i automatic blood cell analyzer immediately,then were split into two parts and stored at 4 ℃ and room temperature respectively.The measurement was repeated in 24 and 48 h,and the groups were compared on WBC,RBC,PLT,HGB,IG％ and IG#.Results There were statistically significant differences between the instantly measuring result and those in 24 and 48 h under room temperature (P＜0.05).In case of 4 ℃,the instantly measuring result had no obvious difference with that in 24 h (P＞0.05),while statistical difference with that in 48 h (P＜0.05).Conclusion Newborns' peripheral blood can be stored at 4 ℃C,and the counting results will not be affected for WBC,RBC,PLT,HGB,IG％ and IG# within 24 h.
ABSTRACT
A prospective study was conducted to evaluate the storage period and sterility of placental blood from neonates delivered by caesarian section. Following caesarian section of the infant and placenta, the placenta was immediately placed in a sterile tray and the umbilical cord near the clamps was rinsed with sterile saline and cut 2 cm below the clamps with sterile scissors. An Fr 8 feeding tube was inserted into an umbilical vein and 43 ml of placental blood was drawn into a 50 ml disposable syringe which contained 7 mls of CPDA-1 solution. Five-ml aliquots of blood retrieved from the placenta were inoculated into aerobic and anaerobic blood culture bottles. Sixty-four specimens of CODA-1 anticoagulant blood were retrieved from the placenta and evaluated for biochemical changes to determine the recommended storage period. The mean plasma potassium concentrations were 4.9 + 1.1, 9.1 + 2.6, 13.1 + 2.0 mM/l at 0, 48 and 72 hours after collection, respectively. The 72-hour potassium concentration was higher than the value in adult whole blood stored for 7 days, which is considered fresh blood and recommended for transfusing newborn infants. The 107 paired aerobic/anaerobic culture specimens showed an overall contamination rate of 13%. These findungs suggest that placental blood in CPDA-1 can be stored for 48 hours for autologous transfusion and that rinsing the umbilical cord in sterile saline cannot prevent bacterial contamination in the retrieved placental blood.