ABSTRACT
Objective@#To study the influence of environmental factors on the two-species biofilm formed by the combinations of Streptococcus oligofermentans (So) with Streptococcus mutans (Sm) and Streptococcus sanguinis (Ss) with Sm so as to evaluate the role of So in maintaining the microecological balance of the oral cavity.@*Methods@#Single-and two-species biofilms were grown on saliva-coated surfaces (glass tube and 96-well plate). Colony-counting method and safranin staining method were used to measure the biofilms formed under various oxygen conditions (aerobic and anaerobic), sucrose conditions (0%, 1% and 5% sucrose concentrations) and pH conditions (5.5, 6.0, 6.5, 7.0, 7.5 and 8.0).@*Results@#Comparing the numbers of Sm in two co-cultures under various conditions, Sm counts in So+Sm group [(7.70±2.46)×108 CFU/ml] were significantly lower than those in Ss+Sm group [(9.00±1.13)×108 CFU/ml] in aerobic environment (P<0.05). Sm counts in So+Sm group [(2.80±0.52)×108 CFU/ml] were also significantly lower than those in the Ss+Sm group [(4.00±1.25)×108 CFU/ml] in anaerobic environment (P<0.05). The Sm counts in So+Sm group [(8.90±0.82)×108 CFU/ml] were significantly higher than those in Ss+Sm group [(7.50±1.73)×108 CFU/ml] in 0% sucrose environment (P<0.05). The Sm counts in So+Sm group [(5.70±2.94)×108 CFU/ml] were significantly lower than those in Ss+Sm group [(10.30±3.21) ×108 CFU/ml] in 1% sucrose environment (P<0.05). The Sm counts in So+Sm group [(6.10±1.71)×108 CFU/ml] were also significantly lower than those in Ss+Sm group [(7.40±1.20)×108 CFU/ml] in 5% sucrose environment (P<0.05). The Sm counts in So+Sm group [(3.50±1.50)×108 CFU/ml] were significantly lower than those in Ss+Sm group [(10.70±2.80)×108 CFU/ml] in pH7.0 environment (P<0.05). Comparing the formation of biofilm after 24 h cultivation, the Sm counts in So+Sm group were significantly lower than those in Ss+Sm group both in aerobic and anaerobic environments (P<0.05). The Sm counts in So+Sm group were significantly higher than those in Ss+Sm group in 0% sucrose environment (P<0.05). The Sm counts in So+Sm group were significantly lower than those in Ss+Sm group in 1% and 5% sucrose and pH 7.0 environments (P<0.05). Both So and Ss had no inhibitory effect on Sm in pH5.5 and pH8.0 environments.@*Conclusions@#In the in vitro two-species co-culture systems, So showed stronger inhibitory effects than Ss on Sm and its inhibitory ability might influenced by various environmental factors.
ABSTRACT
Objective:To study the colonization ability of Streptococcus oligofermentans (S.oligofer-mentan)in the condition of high sucrose in oral cavity of rats.Methods:In this study,48 SPF-SD rats aged 21 days were selected.From 24th to 27th days,the rats were fed with water of antibiotic and fed with high glucose diet continuously.On the 28th day,the rats were divided into four groups randomly,12 rats per group.From the 28th day to 30th day,the first group (SMgroup)was inoculated with S.mutans,the second group (SO group)with S.oligofermentan,the third group (SO+SM group)with mixture of S. mutans and S.oligofermentan,the control group not with any bacteria.On the next day and the 10th day after inoculation of bacteria,the samples of dental plaque of the rats were acquired by scrubbing occlu-sal,buccal and lingual surfaces of bilateral mandibular molars with sterile swabs.The samples of SM group were inoculated on MSB and BHIS,of SO group on MSAE,of SO+SMgroup on MSB,MSAE and BHIS,of the control group on MSB and MSAE.S.mutans were screened and calculated on MSB,the suspected colonies of S.oligofermentan were screened and identified by the analysis of 16S rDNA.Re-sults:On the next day,the detection rate of S.oligofermentan was 33.3% (4/12)in the group of SO;in the group of SO+SM,the detection rate of S.oligofermentan was 0,the detection rate of S.mutans 100.00%,and the proportion of S.mutans 14.70%±4.53%;in the group of SM,the detection rate of S.mutans was 100.00%,the proportion of S.mutans 12.42%±4.27%.On the 10th day,in the group of SO,the detection rate of S.oligofermentan was 0;in the group of SO+SM,the detection rate of S.oligofermentan was 0,the detection rate of S.mutans 100.00%,and the proportion of S.mutans 15.78%±5.10%;in the group of SM,the detection rate of S.mutans was 100.00%,and the propor-tion of S.mutans 17.08%±5.75%.Conclusion:In the condition of the experiment where high glucose was maintained in the oral cavity in rats,S.oligofermentan appeared transiently and couldn’t colonize in the rats.
ABSTRACT
Objective:To investigate the inhibition of Streptococcus oligofermentans(So)on Streptococcus mutans(Sm)and the hydro-gen peroxide(H2 O2 )producibility by So under different sucrose concentration environment.Methods:The inhibition of So on Sm was observed by plating method under different sucrose concentration environment.The initial synthesis rates and production of H2 O2 by So were determined by 4-aminoantipyine-horseradish peroxidase method.Results:Under 500 mmol/L of H2 O2 ,the inhibition of So on Sm was not observed.Under the other sucrose concentration environment,the inhibition of So on Sm was as following:50 mmol/L >0 mmol/L and 1 mmol/L(P 0 mmol/L and 1 mmol/L >500 mmol/L(P 50 mmol/L >500 mmol/L(P <0.05).When So was inoculated before Sm,the inhibition of So on Sm was stronger than that when the two species were inoculated at the same time.Conclusion:The capability of the inhibition of So on Sm and the production of H2 O2 by So are influenced by sucrose concentration.