ABSTRACT
Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.
ABSTRACT
A literatura é unânime em afirmar que o uso de aparelhos fixos ortodônticos para a correção da má oclusão causa uma mudança da microbiota bucal. Há um aumento do acúmulo de placa e de Streptococcus mutans, microrganismo associado à desmineralização do esmalte dentário e desenvolvimento da lesão de cárie. Dependendo do tipo de material dos acessórios ortodônticos, sugere-se uma adesão e colonização bacteriana diferenciada. Diante das divergências encontradas na literatura, este artigo tem como objetivo realizar uma revisão crítica sobre a adesão de microrganismos em aparelhos ortodônticos fixos e sua consequência na cavidade bucal.
It is a common sense in the literature that the use of orthodontic appliances for malocclusion correction causes a change in the oral microflora. There is an increase in plaque accumulation and an enhancement of Streptococcus mutans colonization, which is related to enamel demineralization and caries progression. For each type of material used in the appliances, there will be a specific bacterial colonization. In order to clarify all the divergences found in the literature when colonization is studied, the aim of this paper is to make a critical review of the literature concerning microorganism adhesion in fixed orthodontic appliances and the consequences in the oral cavity.
Subject(s)
Biofilms , Orthodontic Brackets , Streptococcus mutansABSTRACT
The purpose of this study was to evaluate the adherence of Streptococcus mutans to the surface of direct-use composites submitted to different polishing techniques. Cylindrical specimes were made of resins: Filtek Z250®; Filtek A110®; Filtek P60® and Filtek Supreme®. The samples were grouped in accordance with the surface treatment: poliester matrix (without polishing); brush technique (without polishing); Sof-Lex® disks; fine grain and extra-fine grain diamond points; carbide burs with 12 and 30 blades; diamond points associated with silicone points (Enhance® system); and multibladed burs associated with silicon points. In a sterile laminar flux apparatus the samles were disinfected and placed in Petri dishes where BHI sucrose broth was added and S. mutans ATCC 25175 was inoculated in a such a manner that the study surface was turned upwards (incubation at 35+ - 2°C). After 24 and 72 hours, three previously calibrated examiners used a stereomicroscope to classify the samples with regard to the presence and thickness of the pellicle and quantity of colonies. The data were statistically analyzed nonparametric tests of Mann Whitney and Student-New-man-Keuls (p<0,05). The microhybrid resin exhibited the same indexes of adherence when compared to both the microparticle and the nanoparticle, and lower adherence than packable composite.Among the polishing systems, the most effective were the multibladed burs and diamond points used before the silicon points. For the obtainment of a smooth surface by means of polishing, it is necessary to inhibit the impregnation of bacteria on the composite resin restorations.