ABSTRACT
Objective To explore polycyclic aromatic hydrocarbons(PAHs) exposure of traffic policemen in Beijing and its impact on DNA oxidative damage. Methods From Jun. to Aug. 2007,41 male traffic policemen and 34 male suburban inhabitants in Beijing were selected. The ambient fine particulate matter(PM2.5) was sampled within two consecutive days in the work places of traffic policeman and in the residential area of suburban inhabitants respectively . The levels of urinary 1-hydroxypyrene(1-OHP) and 10 PAHs species were both analyzed by high performance liquid chromatography(HPLC) . The levels of urinary 8-hydroxy-2’-deoxyguanosine(8-OHdG) were detected by ELISA kit,while the questionnaires for health information on smoking,drinking alcohol,exercise habit and so on were also inquired. Results The average levels of PM2.5,benzo[a]pyrene [B(a) P] and total PAHs in the traffic policeman group were 0.096 mg/m3,3.20 ng/m3,and 38.32 ng/m3 respectively,while the corresponding values in the suburban inhabitant group were 0.045 mg/m3,1.54 ng/m3 and 25.43 ng/m3 respectively. The concentration of urinary 1-OHP in the policeman group [(0.50 ?0.38 ) ?mol/mol Cr] was higher than that of the suburban inhabitant group([0.34?0.28) ?mol/mol Cr],P0.05. Multiple factors analysis indicated that urinary 1-OHP and smoking habit had influence on urinary 8-OHdG level,while there was no relationship with other factors(such as drinking alcohol,exercise and cooking habit) . Conclusion Traffic pollution may increase the level of DNA oxidative damage in policemen in Beijing.Traffic and smoking habit-related PAHs exposure is the important influencing factor.
ABSTRACT
Objective To assess the exposure to polycyclic aromatic hydrocarbons(PAHs) in traffic police and suburban inhabitants in Beijing and to explore the factors that can influence urinary 1-hydroxypyrene(1-OHP) which will be taken as PAHs exposure biomarker.Methods From Jun.to Aug.2007,Sixty-two traffic policemen and 35 male suburban inhabitants were selected.The ambient PM2.5 samples were collected within two consecutive days in the work place of traffic policeman and in the residential area of suburban inhabitants respectively.The levels of urinary 1-OHP and 10 PAHs species in the collected PM2.5 were determined by high performance liquid chromatography(HPLC),while the information on smoking,drinking alcohol,exercising habit and so on was investigated by questionnaire.Results The average levels of benzo[a]pyrene,pyrene and total PAHs in the traffic policeman group were 3.20,6.48 and 38.32 ng/m3 respectively,while the corresponding values in the suburban inhabitant group were 1.54,4.05 and 25.43 ng/m3 respectively.The concentration of urinary 1-OHP in the policeman group was higher than that of the suburban inhabitant group.In addition,the effect of smoking on the 1-OHP concentrations were also detected in the smoking suburban inhabitants and serious smoking traffic policemen(≥20 cigarettes per day).There was no effect of other factors(such as drinking alcohol,exercising and cooking habit) on 1-OHP.Conclusion Both traffic policemen and suburban inhabitants in Beijing were exposed to high level of PAHs.The ambient air exposure and smoking are the most important factors influencing the concentration of urinary 1-OHP.