ABSTRACT
Abstract The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) and Super EBA(r). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus(r) was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus(r) and EndoSequence Root Repair Material Putty(r). Super EBA(r) showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus(r) and EndoSequence Root Repair Material Putty(r) were less cytotoxic in the highest dilution (1:1) compared with Super EBA(r).
Resumo O objetivo deste trabalho foi avaliar in vitro a citotoxicidade em fibroblastos do ligamento periodontal humano de três cimentos de retrobturação: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) e Super EBA(r). Uma cultura de fibroblastos primários do ligamento periodontal humano foi obtida anteriormente a fim de avaliar a citotoxicidade dos três extratos dos cimentos de retrobturação após 2 e 7 dias de endurecimento. As diluições em série destes extratos (1:1, 1:2, 1:4 e 1:8) foram avaliados em 1, 3 e 7 dias empregando o ensaio colorimétrico metil-tiazol-tetrazólio (MTT). A viabilidade celular foi calculada em base da porcentagem do grupo de controle negativo, que representou 100% de viabilidade de células. As análises estatísticas foram realizadas com o teste t, ANOVA e teste de Kruskal-Wallis a um nível de significância de 5%. Verificou-se que a principal diferença entre os cimentos de retrobturação estava nas diluições mais elevadas (p<0,05) e houve um comportamento semelhante nas diluições mais baixas (p>0,05). A viabilidade celular dos fibroblastos do ligamento periodontal humano foi superior para MTA Angelus(r) de 2 dias de endurecimento (p<0,05), em comparação com os outros materiais de retrobturação. Não houve diferença significante entre MTA Angelus(r) e EndoSequence Root Repair Material Putty(r) de 7 dias de endurecimento. Super EBA(r) mostrou a menor percentagem da viabilidade celular nas diluições mais altas (p<0,05). Portanto, os cimentos de retrobturação MTA Angelus(r) e EndoSequence Root Repair Material Putty(r) foram menos citotóxicos na diluição mais alta (1:1) em comparação com Super EBA(r).
Subject(s)
Humans , Periodontal Ligament/pathology , Root Canal Filling Materials , Cells, Cultured , In Vitro TechniquesABSTRACT
O objetivo deste estudo foi avaliar a resposta biológica de MTA e Super EBA (SEBA) em retro-obturações em cães. Lesões periapicais induzidas foram tratadas por cirurgia parendodôntica com retro-obturação utilizando MTA (n = 12) ou SEBA (n = 12). Após 180 dias, necrópsias foram coletadas, submetidas ao processamento histológico e análise estatística. MTA gerou menor presença de células inflamatórias e maior presença de cápsula fibrosa e de novo cemento em relação ao SEBA. Conclui-se que MTA e SEBA são biocompatíveis, mas apenas o MTA é bioativo com maior capacidade cementogênica.
The aim of this study was evaluate the biological response of MTA and Super EBA (SEBA) in root end filling in dogs. Parendodontic surgery and root end filling were used to treat induced periapical lesions using MTA (n = 12) or SEBA (n = 12). After 180 days, necropsies were collected, submitted to histological processing and statisticalanalysis. MTA induced smaller presence of inflammatory cells and higher presence of fibrous capsule and new cementum than SEBA. It was concluded that MTA and SEBA are biocompatible but only MTA is bioactive with the highest cementogenic capacity.
Subject(s)
Animals , Dogs , Materials Testing , Biocompatible Materials/standards , Root Canal Filling Materials/standards , Root Canal Obturation/methodsABSTRACT
O objetivo deste trabalho foi verificar a adaptação marginal de materias retroobturadores por microscopia eletrônica de varredura (MEV). Utilizou-se 12 pré-molares inferiores epicetomizados, eliminando-se 3.0 milímetros finais de cada raiz e executando-se em seguida os retropreparos com profundidade de 3,0 milímetros mediante o uso de pontas ultra-sônicas. Dividiram-se os espécimes em três grupos de quatro elementos e foram feitas retroobturações com amálgama, Super EBA e MTA, analisadas em aumento de 1000 X para verificação e medição dos gaps eventualmente encontrados entre o material retroobturador e as paredes dentinárias. Os três materiais testados apresentaram falhas na adaptação marginal.
Subject(s)
Dental Amalgam , Dental Marginal Adaptation , Dental Materials , Materials Testing , Microscopy, Electron, ScanningABSTRACT
The purpose of this study was to evaluate the apical sealing ability of Super-EBA, MTA and Dyract-flow as retrofilling materials. Forty-eight extracted human teeth with straight and single root canal were used in this study. The root canals were prepared to a #40 apical canal size and obturated with gutter-percha. Apicoectomies were performed and root end cavities were prepared to a depth of 3mm using an ultrasonic device. The root end cavities were filled with Super-EBA, MTA or Dyract-flow. Leakage was measured using an electrochemical technique for 4 weeks. According to this study, the results were as follows. 1. Increasing leakage with time was observed in all groups. 2. No significant difference was noted among the 3 groups with time (p = 0.216). 3. No significant difference was noted among the 3 groups when measured within the same time interval (p = 0.814). The results of this study suggest that the sealing ability of Dyract-flow is equal to that of Super-EBA and MTA, and Dyract-flow may be an alternative to other materials for root-end filling.