ABSTRACT
Aim To investigate the effect of astragalo- side Ⅳ on focal cerebral ischemia-reperfusion injury in rats.Methods The focal cerebral ischemia/reper-fusion of rat left middle cerebral artery occlusion ( MCAO) was induced by suture method .Male SD rats were randomly divided into sham operation group , cer-ebral ischemia/reperfusion group , astragaloside IV group and solvent control group .Except for the sham operation group , the others were subjected to ischemia 2h and reperfusion 24h.Then, rats with successful model were chosen for the detection of various indexes . Astragaloside IV group was injected intraperitoneally with astragaloside IV(20 mg· kg -1 ) at the same time as reperfusion , while solvent control group was injected with the same amount of solvent .TTC staining was used to detect the volume of cerebral infarction , and Nissl staining to observe the changes of histomorpholo-gy, and transmission electron microscope (TEM) to ob-serve the ultrastructure of the cells .Results There was no neurological deficit in the sham operation group, and the volume of cerebral infarction was zero . Compared with the sham operation group , there were some increased neurological deficits , nerve cell damage and cerebral infarction volume in other groups ( P <0.05) .Compared with the cerebral ischemia/reperfu-sion group , the nerve function damage could be signifi-cantly improved , the damage of neurons reduced , and the volume of cerebral infarction decreased ( P<0.05 ) in astragaloside IV group , and there was no obvious change in the solvent control group ( P>0.05 ) .Con-clusion Astragaloside IV can reduce the focal ische-mia/reperfusion injury in rats and protect nerve cells from damage.
ABSTRACT
@#There are many ways to prepare middle cerebral artery occlusion (MCAO) rat model. The focal cerebral ischemia model es-tablished by the suture-occluded method is widely accepted as an ideal focal cerebral ischemia model, which can be used in the basic and clinical study of cerebral vascular diseases. However, it still has the defects of high technical requirement, large model difference and high animal mortality. A variety of focal cerebral ischemia models were established by changing the retention time of the thread, the road the thread plugging into, the type of thread bolt and the depth of the thread plugging. This article compared different suture-occluded method, as well as the suture-occluded method to other modeling methods, different cerebral ischemia models may be different in the pathophysiology of cerebral infarction, and the effects may also be different.
ABSTRACT
Middle cerebral artery occlusion model with suture-occluded method in rats is widely regarded as the standard animal model of focal cerebral ischemia .In the process of preparation , it is easy to be influenced by multiple factors;among them the successful operation plays a key role .This article summarized from several aspects , such as the anatomic locations of the major arteries in the rat ’ s neck , where to perform the incision , which side to perform the operation and where to insert the suture .