ABSTRACT
Objective: To accelerate the development of nation medicine, ultra-high performance liquid chromatography (UPLC) combined with content analysis of index compound, cluster analysis (HCA), and principal component analysis (PCA) was used to evaluate and discriminate three kinds of national folk medicinal plants, Swertia davidi, S. punicea, and S. angustifolia. Methods: The chromatograms of 27 samples from three kinds of Swertia L. were collected. The chromatograms were imported in Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A to obtain data retention time and peak area of samples. Three sets of samples of similarity were analyzed. The peak area data were imported in SPSS and SIMCA-P+ software for cluster analysis and principal component analysis, dendrogram and principal component scores were obtained, and the clustering effect was observed. Results: The line relationship of this way was good (R2 > 0.9993), with high precision instrument (RSD of 0.19%-2.45%), the method had good reproducibility (RSD of 0-1.77%), recovery was between 95.8% and 102.3% (RSD of 0.45%-2.81%). Three kinds of Swertia L. medicinal plants were different in the contents of index compounds, and the contents of index compounds in the same species were different. Swertiamarin: S. davidi (0.54-10.05 mg/g), S. angustifolia (undetectable), S. punicea (1.76-15.62 mg/g); gentiopicroside: S. davidi (4.27-11.18 mg/g), S. angustifolia (0.26-3.58 mg/g), S. punicea (1.13-16.11 mg/g); sweroside: S. davidi (0.02-0.28 mg/g), S. angustifolia (0.02-0.14 mg/g), S. punicea (0.11-44.52 mg/g); mangiferin: S. davidi (0.14-0.55 mg/g), S. angustifolia (0.03-0.04 mg/g), S. punicea (0.01-13.49 mg/g). The accuracy of dendrogram classification was 85.2%. The contents of index compounds of three false anomalies were less than the remaining nine samples of S. punicea. The effect of discrimination by PCA scores plot of three Swertia L. plants was good, and S. punicea with dispersive distribution indicated that the individuals were significant difference. Conclusion: The method to evaluate and discriminate three Swertia L. medicinal plants is feasible by UPLC combined with analysis of index compound HCA and PCA. In this article, S. punicea is more valuable compared S. davidi and S. angustifolia.
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Objective To observe the protective effect of Swertia Punicea Hemsl. on mice chronic hepatic injury induced by CCI_4. Methods Serum levels of ALT, AST and total protein were measured and the liver index, spleen index as well as hepatic pathological changes were also observed. Results Swertia Punicea Hemsl. obviously decreased the levels of serum ALT and AST(P
ABSTRACT
Objective: to observe the protective effect of Swertia Punicea on experimental hepatic injury induced by CCl4 and BCG/LPS respectively. Methods: Mouse models of hepatic injury were induced by CCl4 and BCG/LPS. Serum levels of ALT and AST, MDA content and the pathological changes of hepatic tissue were observed. Results: Swertia Punicea obviously inhibited the rising levels of serum ALT and AST, improved the histological features and relieved hepatic edema induced by CCl4 in mice. However, it has no obvious action on the rising levels of ALT and AST induced by BCG/LPS. It also reduced MDA content and decreased the hepatic index and the splenic index. Conclusion: Swertia Punicea has antioxidation and a protective action on the chemical and immunological hepatic injury, especially on the chemical hepatic injury.
ABSTRACT
AIM:To observe the protective effect of Swertia Punicea on chronic liver injury induced by CCl 4. METHODS: To measure levels of serum ALT、AST and the total protein, and to observe changes of the liver index、the spleen index as well as the liver pathological change. RESULTS: Swetia Punicea obviously lowered the levels of serum ALT and AST, and increased the content of the total protein. The drug decreased the liver index, but it had no obvious effect on the spleen index. It also improved the liver fibrosis. CONCLUSION: Swertia Punicea can protect liver and decrease enzymatic activity, and can retard or ease up the formation of the liver fibrosis.