ABSTRACT
Objective To study the fingerprints of 15 batches of Mongolian medicinal materials of Lomatogonium rotatum collected from different places by HPLC, and to evaluate the quality of the medicinal materials of L. rotatum by similarity calculation. Methods A total of 15 batches of ribbed flowers were collected by HPLC. Chromatographic column: YMC C18 column (250 mm × 4.6 mm, 5 μm), mobile phase: acetonitrile-0.4% phosphoric acid-methanol, gradient elution, flow rate was 0.8 mL/min, detection wavelength was 254 nm, column temperature was 30 ℃. The similarity of fingerprints was evaluated by using the “Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine 2004A Edition”. Results The fingerprints of L. rotatum of Mongolian medicine were established, 15 common peaks were identified, five common peaks were identified, and the similarity among 15 batches of L. rotatum and the fingerprints of control was in the range of 0.881—0.997. The fingerprints of erect column flowers have good precision, stability and reproducibility. Conclusion The characteristic fingerprint of L. rotatum was established for the first time, which not only provides a new scientific basis for the identification and quality control of L. rotatum, but also has important significance for the quality evaluation of L. rotatum.
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OBJECTIVE： To establish the qualitative and quantitative control method of Tibetan medicine Thlaspi semen. METHODS： TLC and HPLC method were used to identify and determine flavonoids isovitexin， swertisin and glucosinolates sinigrin from 15 batches of T. semen. The stationary phases identified by TLC of flavonoids and glucosinolates were polyamide film and high performance silica gel GF254. The developing agents were trichloromethane-methanol-glacial acetic acid （11 ∶ 1 ∶ 1，V/V/V） and ethyl acetate-methanol- triethylamine （4 ∶ 5 ∶ 0.5，V/V/V）. In chromatogram condition of content determination of isovitexin and swertisin， the separation was performed on CAPCELL PAK MGⅡ C18 column with mobile phase composed of acetonitrile-0.4％ glacial acetic acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 336 nm. In chromatogram condition of content determination of sinigrin， the separation was performed on CAPCELL PAK MGⅡ C18 column with mobile phase composed of acetonitrile-0.02 mol/L tetrabutylammonium hydrogen sulfate （15 ∶ 85，V/V，pH 6） at the flow rate of 1.0 mL/min. The detection wavelength was set at 227 nm. RESULTS： In TLC identification chromatogram， spots corresponding to isovitexin， swertisin and sinigrin control were detected in test samples. The linear ranges of isovitexin， swertisin and sinigrin were 1.26-79.00， 1.21-75.38， 12.80-640.00 μg/mL， respectively （all r≥0.999 5）. The limits of detection （LODs） were 0.09， 0.12， 0.15 μg/mL， and limits of quantitation （LOQs） were 0.39， 0.43， 0.54 μg/mL， respectively. RSDs of precision， stability （24 h） and reproducibility tests were all lower than 2.0％（n＝6）. The recoveries were 99.1％， 97.0％ and 98.1％， and RSDs were 1.9％， 1.8％， 1.8％（n＝6），respectively. The contents of isovitexin， swertisin and sinigrin in 15 batches of T. semen were 0.013-0.090， 0.020-0.130 and 18.92-40.75 mg/g， respectively. CONCLUSIONS： Established quality control method is simple， reproducible and stable， and can be used for the quality control of Tibetan medicine T. semen.
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Objective: To study the chemical constituents from the whole plant of Swertia davidi. Methods: The chemical constituents were isolated and purified with silica gel column chromatography, silica gel chromatography, etc. The structures of the compounds were identified by physicochemical properties and various spectroscopic methods. Results: Sixteen compounds were isolated as uvaol (1), oleanolic acid (2), 5-carboxyl-3, 4-dihydrogen-1H - 2-benzopyran-1-one (3), 3β, 12β-pregnane-16-12-ene-20 (4), swertisin (5), 6-C-β-glucopyranosyl-7-O-methylluteolinidin (6), isoscoparin (7), 6-demethoxy-7-methylcapillarisin (8), 3-acetoxy-28-hydro-xyl-12-eneurs (9), gentiopicroside (10), N-pentacosy-2-carboxy-benzoylamide (11), 4-O-glycosyloxy-2-hydroxy-6-methoxy-acetophenone (12), quercetin-3-O-β-D-xylopyranosyl-(1→2) - β-D-galactopyranoside (13), 8-epikingiside (14), 3, 5-dicaffeoylquinicacid (15), and 2-methylphenyl-1-O-β-D-glucopyranoside (16). Conclusion: All the compounds are isolated from S. davidi for the first time.
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Objective: To study the chemical constituents from Liparis nervosa. Methods: The column chromatography on silica gel, MCI gel, ODS, and Sephadex LH-20 columns was applied for the isolation and purification of the chemical constituents. The structures were identified based on physicochemical property and spectral data. Results: Nine compounds were isolated and identified as (hexahydro-1H-pyrrolizin-1-yl) methyl-2, 2-dimethyl-8-(3-hydroxyisoamyl) chroman-6-carboxylate (1), friedelin (2), α-spinasterol (3), p-hydroxybenzaldehyde (4), swertisin (5), 6, 8-di-C-α-L-arabinopyranosyl apigenin (6), 6, 8-di-C-α-L-arabinopyranosyl genkwanin (7), 8-C-α-L-arabinopyranosyl apigenin (8), and thymidine (9). Conclusion: Compound 1 is a new compound named nervosine B, and compounds 2-9 are isolated from L. nervosa for the first time.