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Objective To investigate the relationship between human papillomavirus (HPV) infection and cervical lesions, and to explore the application value of HPV typing in cervical disease screening. Methods Colposcopic examination and cervical biopsy were performed in 1 851 cases of HPV positive patients from January 2017 to September 2018 in the cervical disease clinic of Shanghai First People's Hospital.Pathological diagnosis was used as the gold standard to analyze the distribution of HPV subtypes and the relationship between HPV subtypes and cervical lesions. Results Among 1 851 patients, high-risk human papillomavirus (HPV) infection was the main type.The detection rate of HPV 16 was the highest, followed by HPV52, HPV58, HPV53 and so on.A total of 234 patients with positive biopsy results were found, and 77 patients with CIN2 or above lesions were detected.The ratio of CIN2 or above patients with HPV 16 was 11.8% (45/380), higher than 2.4% (32/1 328) of other high-risk subtypes such as HPV52 and HPV58.The difference was statistically significant(χ2=61.60, P < 0.001).In the age factor, the high-risk positive ratio of HPV in the 35-year-old group was 96.91% (1 225/1 264), higher than 94.38% (554/587) in the 35-year-old group.The difference was statistically significant (χ2=6.898, P=0.009).The detection rate of CIN1 disease was 12.64% (234/1 851) in HPV classification test, 8.59% (159/1 851) in liquid based thin layer cytology test (TCT), and the detection rate of TCT as a screening method was lower than that of HPV test method (χ2=16.01, P < 0.001). Conclusion The survey find that HPV subtypes are the most common in the order of HPV16, HPV52, HPV58, HPV53 and HPV18.HR-HPV infection was closely related to cervical lesions, and with the increase of high-risk infections, the degree of cervical lesions also increased.The detection rate of HPV typing is higher than that of TCT screening for cervical lesions.HPV genotyping has high sensitive and negative predictive value in screening cervical lesions.
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Objective To investigate the relationship between human papillomavirus (HPV) infection and cervical lesions, and to explore the application value of HPV typing in cervical disease screening. Methods Colposcopic examination and cervical biopsy were performed in 1 851 cases of HPV positive patients from January 2017 to September 2018 in the cervical disease clinic of Shanghai First People's Hospital.Pathological diagnosis was used as the gold standard to analyze the distribution of HPV subtypes and the relationship between HPV subtypes and cervical lesions. Results Among 1 851 patients, high-risk human papillomavirus (HPV) infection was the main type.The detection rate of HPV 16 was the highest, followed by HPV52, HPV58, HPV53 and so on.A total of 234 patients with positive biopsy results were found, and 77 patients with CIN2 or above lesions were detected.The ratio of CIN2 or above patients with HPV 16 was 11.8% (45/380), higher than 2.4% (32/1 328) of other high-risk subtypes such as HPV52 and HPV58.The difference was statistically significant(χ2=61.60, P < 0.001).In the age factor, the high-risk positive ratio of HPV in the 35-year-old group was 96.91% (1 225/1 264), higher than 94.38% (554/587) in the 35-year-old group.The difference was statistically significant (χ2=6.898, P=0.009).The detection rate of CIN1 disease was 12.64% (234/1 851) in HPV classification test, 8.59% (159/1 851) in liquid based thin layer cytology test (TCT), and the detection rate of TCT as a screening method was lower than that of HPV test method (χ2=16.01, P < 0.001). Conclusion The survey find that HPV subtypes are the most common in the order of HPV16, HPV52, HPV58, HPV53 and HPV18.HR-HPV infection was closely related to cervical lesions, and with the increase of high-risk infections, the degree of cervical lesions also increased.The detection rate of HPV typing is higher than that of TCT screening for cervical lesions.HPV genotyping has high sensitive and negative predictive value in screening cervical lesions.
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Objective: To identify appropriate ways of applying the current cervical screening methods to minimize the occurrence of missed diagnosis of high-grade cervical intraepithelial neoplasia (CIN2/3) and cervical cancer. Methods: A retrospective analysis was conducted based on the clinical data of 318 patients, who had CIN2/3 or cervical cancer and were treated in Aviation General Hospital Affiliated to China Medical University between June 2014 and August 2018. Among these cases, 296 involved CIN2/3, and 22 involved cervical cancer. All of the patients involved underwent a thinprep cytology test (TCT) and high-risk human papilloma virus (hrHPV) screening, and their age and TCT and hrHPV results were further analyzed. Results: Among the 296 patients diagnosed with CIN2/3, 130 of them (43.92%) were aged between 30 and 39 years, ranking the top among all age groups. Sixty-nine young patients (23.31%) aged between 20 and 29 years were diagnosed with CIN2/3, ranking in the third place. Among all of the 318 cases, the TCT results were positive in 199 cases (62.58%), hrHPV results were positive in 308 cases (96.86%), and there were 313 cases (98.43%, 313/318) wherein both TCT and hrHPV results were positive according to joint screening. The most predominant hrHPV subtypes were 16, 52, 58, 33, 18, and 31. Conclusions: As an increasing number of young women are diagnosed with CIN2/3 and cervical cancer, their screening should not be ignored. Single use of TCT screening leads to more frequent missed diagnosis of high-grade lesions compared with single use of hrHPV screening, while joint use of both screening methods could improve the detection rate. Even if TCT results were negative, it is suggested to conduct a colposcopy for those patients with high-risk subtypes besides hrHPV16 and 18 positivity, and in particular, for patients with hrHPV 52,58,33, and 31 positivity.
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Objective To study the value of TCT, human papillomavirus DNA combined with colposcopy in the early screening of cervical cancer. Methods The clinical data of 80 cases of patients with cervical lesions in our hospital from January 2011 to January 2017 were retrospectively analyzed. Results Among the 80 cases of patients, ASCUS was detected by TCT 8 cases, LSIL6 cases, HSIL4 cases, SCC2 cases, the detection rate of cervical lesions was 25.0% (20/80); Normal and inflammatory 60 cases, accounting for 75.0% (60/80) of the total. HPV-DNA positive 28 cases, negative 52 cases, the positive rate was 35.0% (28/80); The detection rate of TCT (+) HPV (+) was significantly higher than TCT (+) HPV (-), TCT (-) HPV (+), TCT (-) HPV (-) (P<0.05), the detection rate of SCC reached 100.0%; The detection rate of CIN I, CIN II, CIN III of TCT examination, HPV-DNA examination combined with colposcopy were significantly higher than TCT+HPV-DNA examination (P<0.05), but the difference of detection rates of SCC between the two was not significant. Conclusion The value of TCT, human papillomavirus DNA and colposcopy in the early screening of cervical cancer is higher, so is worthy of promotion and use in the clinical.
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Objective To evaluate the diagnostic value of thinprep cytologic test (TCT) combined with high-risk human papillomavirus (HR-HPV) DNA for cervical cancer (CC). Methods 141 cases of patients with abnormal cervical lesions were abnormal examined by TCT and were graded by the results of TCT and cervical biopsy, the HR-HPV-DNA was detected by surface plasmon resonance (SPR). Results The cervical biopsy positive rate 65.2% (92/141) was significantly higher than the positive rate of TCT 39.0% (55/141) (χ2=19.45, P < 0.05). The positive rate of HR-HPV-DNA was 66.0% (93/141) was significantly higher than the positive rate of TCT 39.0% (55/141), (χ2=20.53, P < 0.05). Conclusion TCT, HR-HPV-DNA and cervical biopsy are important clinical diagnostic methods for cervical lesions, combine detection of TCT and HR-HPV-DNA can improve the detection rate of cervical lesions.
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<p><b>OBJECTIVE</b>This study was designed to determine the prevalence of oncogenic human papillomavirus (HPV) in cervical infections in Beijing, China, and to investigate the odds ratio (OR) of HPV single and multiple infections in abnormal cytology.</p><p><b>METHODS</b>A total of 19,018 specimens from outpatients in the department of obstetric and gynecology were collected. They were detected using high-risk HPV genotyping real-time polymerase chain reaction (PCR) kit and analyzed by ThinPrep cytology test for cervical pathological diagnosis. HPV prevalence, age-specific prevalence, and OR of each type of HPV in abnormal cytology were analyzed.</p><p><b>RESULTS</b>Overall, 19.1% (3,623/19,018) of the individuals were positive for HPV infection, 14.9% (2,833/19,018) were positive for a single HPV type, and 4.2% (790/19,018) were positive for multiple types. Among the 3,623 HPV-positive individuals, the most predominant HPV types were HPV52 (4.4%, 834/19,018), HPV16 (3.7%, 710/19,018), and HPV58 (3.4%, 644/19,018). The OR of multiple infections and single infection differed significantly among disease severities. The OR of dual infection was higher than that of each of the two single infection types, respectively.</p><p><b>CONCLUSION</b>HPV prevalence in the outpatients was 19.1%, and the most predominant HPV types in the study were HPV52, HPV16, and HPV58. Women with multiple infectionswere more likely to have abnormal cytology.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Middle Aged , Young Adult , Beijing , Genotype , Papillomaviridae , Classification , Genetics , Papillomavirus Infections , Pathology , Virology , Uterine Cervical Neoplasms , Pathology , VirologyABSTRACT
The tendo calcaneus is a vital structure in the bipedalic system of human propulsion. Its thickness (TCT) was assessed in an apparently healthy young adult population of Southeast Nigeria using ultrasound. The relationship between TCT and other anthropometric parameters was also evaluated. 120 young adults with age ranging between 19 and 30 years volunteered for the study and their TCT was assessed using B-mode ultrasound. Height, weight, foot length and midcalf circumference were measured while Body Mass Index (BMI) and Body Surface Area (BSA) were calculated for the sample population. Results show that the mean values of TCT were 6.26±0.56mm and 6.32±0.58mm for the right and left sides respectively. TCT correlated positively with all the anthropometric variables assessed except age and BMI. TCT also showed significant sexual dimorphism (P=0.000), laterality and bilateral asymmetry with the left being thicker (P=0.000). These findings will further emphasize the biomechanical relationships of the large tendon. The study is the first to report lateralization and significant sexual dimorphism in a young adult population.
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Objective To explore the role of thinprep cytology test(TCT)and HPV-DNA hybrid capture detection played in the screening of cervical lesions.Methods Do TCT examination on 9260 outpatients and do HPVDNA hybrid capture detection on 840 cases therein,836 positive cases continued to have biopsy by colposcope,conformed to the gold standard of histopathology and contrast with cytology in the meantime.Results 8374 cases were negative in the TCT examination;836 cases were cytological abnormal.Cytology-positive rate was 9.03％,ASC-US 398 were 4.29％,LSIL 239 were 2.58％,HSIL 179 are 1.93％,SGUS 9 are 0.097％,SCC 11 are 0.12％.The group of 30 ～40 ranked the highest in cytological positive,it's 43.66％,the second highest was the group of 40 to 50,it's 27.39％.The results of other groups were as follow:＜ 30 is 2.85％,50 ～ 60 is 5.14％,＞ 61 is 0.96％ The difference of them was significant ( P ＜ 0.01 ).HPV infection rate was 27.98％ (235/840).In ASC-US,the detectable rate of CIN in the HPV-positive group was 81.58％ (31/38),the HPV-negative group was 8.98％ ( 15/167),there was significant differene between the two methods(P ＜ 0.01 ).In LSIL,the detectable rate of CIN in the HPV-positive group was 95.45％ (21/22).In HSIL,the detectable rate of CIN in the HPV-positive group was 100％ (16/16).The infection rate in different age group is different,the difference was significant ( x2 =25.2 P ＜ 0.01 ) ; 836positive cases had biopsy by colposcope,507 cases were diagnosed to be CIN,the detectable rate was 60.65％ (507/836).To organize pathology as control,the diagnose accordance rate of cytology and pathology of LSIL and HSIL and SCC were 50.46％,82.12％,100％ (P ＜0.01 ).Took their accordance rate into comparison,the differene was significant.Conclusion Cervical lesion was collected in sex activity period,the peak was 31-40 years.TCT is propitious to screen cervical cancer and precancerous lesion,combined with HPV-DNA hybrid capture detection,colposcopy and pathology,can improve the diagnostic accuracy and the detective rate of CIN and cervical cancer.
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Objective: Cervical smear screening for abnormal cells plays an important role in reducing the mortality of cervical cancer. However, all smears must be approved by the naked eyes using microscopes, and there has been a higher false-positive rate and false-negative rate. Methods: To solve this problem, the thin-layer cytology assistant screening system was developed. On the hardware side, the traditional computer-aided analysis system was improved; and on the software side, gray level transformation, edge detection, image mosaic, and some other image processing methods were introduced; and by the use of neural network, the system could realize the function of acquisition, mosaic, process, measure and recognition of the liquid-based cytology image. Results: This system was brought into the test of 150 thin-layer cytology technology(TCT) cervical cell samples, and the agreement between the results of computer-aided analysis and the manual analysis by pathology experts exceeded 90%. Conclusion: Both the traditional Pap smear and TCT preparation smear can be diagnosed by this system. As a result, the false-positive rate and false-negative rate of the cervical smear screening will be significantly reduced, meanwhile, work efficiency of pathologist can be greatly improved.
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Objective To study the relativity analysis of abnormal cervical pathology results in cytology and histology.Methods With retrospective analysis of 31,634 cases of fluid-based thin-layer method(ThinPrep Cytology Test,TCT)of PUMC Hospital from January,2001 to March,2003,which reported in the Bethesda System,we checked the abnormal results and advised different diagnose biopsy of vaginoscopy and/or conization,and got the relativity description of abnormal results in TCT and CINⅢ/CIS results validated by vaginoscopy,match analysis of the CINⅡ～Ⅲ and CINⅢ/CIS results validated by vaginoscopy and conization labeled by the age group,and relativity analysis of abnormal results in TCT and CINⅢ/CIS results validated by vaginoscopy.Results Among 31,634 cases of TCT test,948 cases had confirmed biopsy results validated by vaginoscopy,of which 70 cases were of CINⅡ～Ⅲ,56 cases were of CINⅢ/CIS.The risk ratio(RR)of different abnormal TCT results in predicting CINⅢ/CIS results validated by vaginoscopy is:ASCUS group,14.7(95% confidence interval 8.0～27.0,P=0.00);CINⅠ group,13.9(6.3～30.9,P=0.00);CINⅡ group,44.2(15.5～126.5,P=0.00);CINⅢ group,272.2(161.6～458.6,P=0.00);Cancer group,unmeasured.As noted,there is no significant difference between the RR of ASCUS group and CINⅠ group(P=0.951)in predicting CINⅢ/CIS results.Conclusions Vaginoscopy examination and biopsy could verify histology abnormity of CINⅡ～Ⅲ及CINⅢ/CIS from abnormal results of TCT,and has a good accordance along with biopsy results of conization.There are significantly greater risk of being CINⅢ/CIS validated by vaginoscopy in the abnormal TCT patients,among which ASCUS group and CINⅠ group have the coequal risk.
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CIN1.Results There are 69 positive cases in ≥CIN1 group,positive rate is 34.5%;There are 16 positive cases in cervicitis group,positive rate is 8.0%.these two groups have statistic difference.Conclusions High risk type HPV16/18 infection correlate with CIN.Patients with high risk type HPV have higher risk of CIN,the High risk type HPV16/18 test is important in cervical disease.If join to TCT,colposcope and pathologic diagnosis,detectable rate will improve.
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Objective The a seek in ideal of iatrotechnique is treatment of Traumatic Cerebral Tinnitus(TCT).Methods Use the pioneering of Laser Meridian Points Therapy(LMPT).The domestic He-Ne laser therapeutic instrument was used with optical fiber wave length 632.8nm,output power 2~30mW,therapeutic power quartz optical fiber at the end of fiber power 1~10 mW,diameter of optical patch at the end of the optical fiber 0.2cm,optical patch area 0.0314 cm2,power density 31.85~318.47mW/cm2.Use the optical fiber end of the instrument to contact and irradiated the following points:Yifeng(SJ 17),Yiming(Extra),Fengch'ih(GB 20),Yingyou,T'ingkung(SI 19),T'inghui(GB 2),Erhmen(SJ 21),Paihui(Du 20),et al.Each point each time was irradiated for 5~15 minutes of laser dosage(energy density)9.555~286.623J/cm2,once a day,7~14 days a course of treatment.Results 262 cases(503 ears)TCT(patients)of 215 cases(82.06%)414 ears(82.31%)showed tinnitus disapperance,45 case(17.18%)86 ears(17.10%)showed marked or some therapeutic effectiveness,2 case(0.76%)3 ears(0.59%)was ineffective.Follow-up survey 1~9 years of 157 cases 284 ears,found 141 case(89.81%)255 ears(89.78%)had their tinnitus disappeared,15 case(9.55%)27 ears(9.51%)showed marked effectiveness or some effectiveness,1 cases(0.64%)2 ears(0.70%)was ineffective.Conclusions LMPT for the treatment of TCT is the pioneering technique we have developed.Better than any other therapies at present and having good short-term effectiveness and stable and ideal long-term effectiveness.LMPT is worthwhile for further study and popularized clinical application.
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Objective:To study the value of ZoomScope colposcopy in diagnosis of cervical diseases.Methods:378 patients with cervical diseases were examined by ZoomScope colposcopy (WALLACH 906043-SP type) from June 2002 to April 2003.The results of 378 patients by colposcopy were compared with Thin-prep Cytology Test (TCT) and histology diagnosis.Results:Colposcopy found normal transformation zone in 114 cases,cervical condyloma in 68 cases,aceto-white epithelium in 95 cases,twin signs in 65 cases,triple signs in 20 cases,cervical cancer in 4 cases,while unsatisfactory colposcopic findings accounted for l2 cases.The accuracy,specificity and sensitivity of colposcopy in evaluation of cervical diseases were 80.56%,74.60%,and 97.48%respectively.The accuracy of colposcopy in diagnosis of cervical diseases were 88.36% and 80.56% respectively as compared with TCT,histology diagnosis,and that of cervical cancer was 100.00% as compared with histology examination.Conclusion:ZoomScope colposcopy in diagnosis of cervical diseases can be accurate,reliable.When colposcopy is combined with TCT and to bospy for cervical diseases,the diagnostic accuracy rate of the precancerous lesions or earlier cervical cancer can be further improved.
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By analysing the ribosome gene(rDNA) sequences in externally transcribed spacer(ETS) of wheat T.controversa(TCK) and its similar spacies T.caries(TCT)and T.foetida(TFL).The special sequences of TCK's ETS have been found,.And designed Taqman probe according to the special sequences,TCK has been successfully detected by using Real-time Fluorescent PCR.