Teratocarcinoma con componente de coriocarcinoma / Teratocarcinoma with a component of choriocarcinoma

Los teratocarcinomas representan aproximadamente entre el 3-5% de los tumores intracraneales en la infancia. Su manifestación clínica puede variar desde un estado asintomático hasta un amplio espectro de síntomas neurológicos, visuales y endocrinos. Se presenta el caso de una paciente femenina de 9 años de edad atendida en el Hospital Pediátrico "Pepe Portilla" de Pinar del Río, por un cuadro de cefalea, vómitos y pérdida brusca de la visión, a la cual se le realizan varias investigaciones y se le diagnostica un teratocarcinoma con componente de coriocarcinoma. Se opera y logra una evolución satisfactoria.

Teratocarcinomas represent 3-5% of the intracranial tumors in childhood approximately. Their clinical manifestations can vary from an asymptomatic status to a wide spectrum of neurological, visual and endocrine symptoms. A 9 year-old female patient presenting headache, vomits and sudden loss of vision attended to "Pepe Portilla" Children Hospital, Pinar del Rio. Several medical examinations were performed, diagnosing a Teratocarcinoma with a component of choriocarcinoma; the patient underwent a surgery having a satisfactory evolution.

CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell

Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.

Impacts of specific TDGF-1 gene silience by siRNA on invasion and migration of human breast cancer cell / 中华内分泌外科杂志

Objective To study the impact of TDGF-1 gene silience by small interfering RNA(siRNA)on the invasion and migration of human breast cancer cell. Methods 3 siRNA fragments were designed according to the characteristic of TDGF-1 gene sequence and the most appropriate siRNA was selected by fluorescence real-time quantitative RT-PCR method. After the human breast cancer cell line MDA-MB-468 was transfected by the selected TDGF-1 siRNA, mRNA and protein of TDGF-1 were determined by real time quantitative RT-PCR and western blot respectively. The migration and invasion ability of the cancer cell were evaluated by wound-healing assay and Boyden chamber model respectively. Results siRNA could down-regulate the level of mRNA and protein of TDGF-1 in a dose-and time-dependent manner. In vitro experiment showed that TDGF-1 siRNA transfection can effectively inhibit the clonal growth, invasion and migration of breast cancer cell in a dose-dependent manner. Conclusions TDGF-1 gene may play an important role in the migration and invasion of human breast cancer cells. siRNA transfection can inhibit the invasion of human breast cancer cells.

Effects of teratocarcinoma-derived growth factor-1 on invasion of human pancreatic cancer cell line PANC1 / 中华胰腺病杂志

Objective To investigate the effects of the silence of teratocarcinoma-derived growth factor-1 ( TDGF-1 ) gene on invasion of human pancreatic cancer cell. Methods Three small interfering RNA (siRNA) targeting for TDGF-1 genes (S1, S2, S3 ) were designed and established, then the gene with the best silencing effects was screened. Human pancreatic cancer cell line PANC1 were transfected by siRNA with different concentrations (3. 125, 6.25, 12.5 nmoL/L), the cells without transfection, and simply treated with liposomes were controls. The expressions of mRNA and protein of TDGF-1 were determined by real time PCR and Western blot assay, respectively. The anchorage-independent growth was examined by clon formation in soft agar, and invasion ability was evaluated by boyden chamber model. PANC1 cells with transfection for 48h were injected into the nude mice to evaluate the invasion ability in vivo. Results The expressions of TDGF-1 mRNA and protein of cells transfected by siRNA were decreased in a dose-and time-dependent manner, which were significantly lower than those in liposomes group. Number of colony formation and transmembrane cell were 19.8 ± 2.2 and 49.8 + 2.6 in the control group, and 5.6 + 1.2 and 8. 1 + 1.1 in the 12.5 nmol/L transfection group. The volumes of tumor 4 weeks after transplation in the control group, liposomes group and the 12.5 nmol/L transfection group were (2.228 ± 0.016 ) cm3, ( 2.186 ± 0.028 )cm3 and ( 0.728 ± 0.023 )cm3. Conclusions TDGF-1 gene silence could inhibit invasion ability of human pancreatic cancer cell PANC1.

Effects of cripto siRNA on liver metastasis of colorectal cancer cell in nude mice / 复旦学报（医学版）

Objective To study the effects of cripto on migration, invasion, and liver metastasis of colorectal cancer cell. Methods After human colorectal cancer cell line SW480 was transfected by cripto small interfering RNA (siRNA), the mRNA and protein level were determined by Real-time RT-PCR and Western blot, respectively. The migration and invasion ability were evaluated by wound-healing assay and boyden chamber model, respectively. Thirty nude mice model of liver metastasis from colorectal cancer was established by splenectomy. Results The siRNA could down-regulate the level of mRNA and protein of cripto in a dose- and time-dependent manner. Suppression of cripto expression could inhibit migration and invasion ability of human colorectal cancer cell in vitro. The metastastic rate and tumor nodules were lower in transfection with cripto siRNA than in two control groups in vivo. Conclusions Cripto gene might play an important role in regulation of liver metastasis from colorectal carcinoma cell, and suppression of cripto gene by siRNA can inhibit liver metastasis of colorectal cancer.

A Case of Teratocarcinoma with Central Diabetes Insipidus / 소아과

Central diabetes insipidus is a rare disorder that can result as a consequence of diverse etiologies, including malformations, autoimmune, infiltrative(e.g. neoplastic or histiocytosis) or traumatic processes, as well as mutations in the gene encoding arginine vasopressin. Idiopathic central diabetes insipidus is a diagnosis of exclusion, one that has been made less frequently through the decades. Idiopathic central diabetes insipidus in children and adolescent requires a frequent follow-up regimen using serial brain MRI and CSF examinations especially if an isolated pituitary stalk thickening or loss of a hyperintense signal in the posterior lobe is observed. Also, so-called "idiopathic" central diabetes insipidus warrants close follow-up to determine the etiology, especially if anterior pituitary hormone deficiencies are detected. We report a case of idiopathic central diabetes insipidus with growth hormone deficiency and loss of a hyperintense signal in the posterior lobe of pituitary in the brain MRI. We followed up with serial contrast enhanced brain MRI and CSF evaluation for the early detection of an evolving occult hypothalamic-stalk lesion and finally detected a newly developed teratocarcinoma in the suprasellar region.

The imaging analysis of intracranial malignant teratomas / 中华放射学杂志

Objective To analyse the imaging characters of intracranial malignant teratomas (IMT). Methods The imaging appearances of IMT were analyzed in 15 pathologically proved IMT. MRI were performed on all the 15 cases, CT on 8 of them. Results Thirteen IMT showed as a lobular cyst solid mass with clear borders. The solid portion of IMT displayed iso (6/13) to hypointense(7/13) on T 1WI and iso (2/13) to hyperintense(11/13) on T 2WI. The heterogeneous enhancement was found in 11 cases, homogeneous in 2. Two IMT exhibited as the intrasellar solid lesions involving thalamencephalon. Calcification was found in 8 IMT on CT scan. No fat was demonstrated in the tumor on both CT and MR. Conclusion IMT displays relatively typical appearances on imaging studies. Together with the CT scan, The MR study may make the diagnosis before the operation.

Primary Teratocarcinoma of the Pineal Gland: A case report

Teratocarcinoma is a form of mixed germ cell tumor composed of a mixture of teratoma and embryonal carcinoma. It is rarely found in the brain, but when present it is most commonly found in the pineal region. We investigated a case of primary teratocarcinoma in the pineal region. The patient was a 10 year old boy who had suffered from a bitemporal headache for one month. Brain CT and MRI revealed a rather well defined ovoid heterogenous mass in the pineal gland region, measuring 4.3 x 3.8 x 3.0 cm in size. Microscopically the tumor contained areas of immature teratoma and embryonal carcinoma. Immunohistochemical staining revealed positive reactions for cytokeratin, epithelial membrane antigen, carcinoembryonic antigen, and alpha-fetoprotein in the embryonal carcinoma component.

Induction of Embryonal Teratocarcinoma Cell(P19 cell) to Differentiate into Smooth Muscle Cell

BACKGROUND: Understanding the regulatory mechanisms of the smooth muscle cells differentiation is one of the central issues in researches of atherogenesis where smooth muscle cells undergo dedifferentiation and regain embryonic phenotype. Smooth muscle myosin heavy chain isoforms(SM1,SM2) are important molecular markers to define the stage of smooth muscle cell differentiation. METHODS: In order to establish an in vitro model of smooth muscle cell differentiation using a pluripotent murine embryonal teratocarcinoma cell line(P19 cell), we first isolated cDNA clone of mouse SM1 and then tried various chemicals to induce P19 cells to differentiate into smooth muscle cells, The expression of Sm1 and alpha-smooth muscle actin was examined using RNase protection assay, Western blotting and indirect immunofluorescence. RESULTS: In the presence of luM retinoic acid, a small proportion of P19 cells could be in duced to differentiate into smooth muscle cells expressing SM1 as well as alpha-smooth muscle actin since day 8 after treatment. By blocking the Brain-2 expression, thus inhibiting neuronal differentiation, we could obtain more abundant differentiated smooth muscle cells. Sequential immunofluorescencc demonstrated that it took weveral days for smooth muscle myosin heavy chain to organize completely in differentiation smooth muscle cells. On the other hand, 10nM retinoic acid, 1% dimethyl sulfoxide or 2mM hexamethylene bisacetamide could not indduce P19 cell to differentiate to smooth muscle cells. CONCLUSION: In conclusion, P19 cells can be induced to differentiate into smooth muscle cells and this in vitro system will be useful in understanding the regulation of SM1 gene expression as well as of angiogenesis.

A Huge Teratocarcinoma in Abdominal Testis / 대한비뇨기과학회지

Cryptorchid testis is correlated with high incidence rate of testicular neoplasm. A 44 year-old male was admitted to our hospital due to huge abdominal mass and dyspnea. Physical examination revealed impalpable right testicle in the scrotum. Abdominal CT scan revealed a huge right retroperitoneal mass with central necrosis. AFP and BHCG were increased. 4 courses induction PVB therapy was performed under diagnosis of unresectable bulky stage IIB nonseminomatous germ cell tumor. Thereafter the tumor size and markers were decreased. But the patient was lost for follow-up. One year later, the tumor size and markers were increased Retroperitoneal excision of intraabdominal mass was performed followed by adjuvant PVB therapy due to persistent increased markers.

A Huge Teratocarcinoma in Abdominal Testis / 대한비뇨기과학회지

Cryptorchid testis is correlated with high incidence rate of testicular neoplasm. A 44 year-old male was admitted to our hospital due to huge abdominal mass and dyspnea. Physical examination revealed impalpable right testicle in the scrotum. Abdominal CT scan revealed a huge right retroperitoneal mass with central necrosis. AFP and BHCG were increased. 4 courses induction PVB therapy was performed under diagnosis of unresectable bulky stage IIB nonseminomatous germ cell tumor. Thereafter the tumor size and markers were decreased. But the patient was lost for follow-up. One year later, the tumor size and markers were increased Retroperitoneal excision of intraabdominal mass was performed followed by adjuvant PVB therapy due to persistent increased markers.

Adenocarcinoma arising in Sacrococcygeal Teratoma: A case report

Authors experienced a rare adenocarcinoma arising in presacral sacrococcygeal teratoma in a adult and herein reported. The patient was a 52 year old female admitted because of lower abdominal pain for 5 to 6 months. Past and familial history were unremarkable. On rectal examination a mass was palpated 3 cm above the anal verge. The large bowel was displaced by an extrinsic mass but there was no evidence of intrinsic tumor lesion on barium enema, CT was done and showed a large presacral tumor attached to the sacrum. Clinical impression was sacrococcygeal teratoma. Resected tumor mass was a relatively well circumscribed cystic mass, measuring 11 x 11 cm and the cystic content was previously evacuated. Inner surface showed ragged appearance, and focal nodular solid area was noted. On microscopic examination, the tumor revealed the derivatives of three germ layers and main components were tissues of respiratory tract. Sections from the solid area showed a well differentiated adenocarcinoma and with stromal, capsular and perineural invasion. Borderline malignant epithelial lesions are also noted suggesting the pathogenesis and progression of this tumor. There was no recurrence for one year after surgery in the follow up.

Teratocarcinoma of Testis: A Report of a Case and Review of Literature / 대한비뇨기과학회지

The first case of testicular teratocarcinoma in Korea, found in 24 year old service man was reported. Complication of the metastases to various organs, gynecomastia and hydrocele was seen at the first examination and simple orchiectomy and delayed x-ray deep therapy were not effective to survive this case. The patient died 3 months after the affliction. Autopsy revealed matastases were to lung, spleen, lower poles of both Kidneys, mediastinal and lumbar paraaortic lymph nodes and histopathological examination showed the teratocarcinoma composed of seminoma, embryonic carcinoma and chorioepitheliomatous elements. Additionally, 13 cases of testis tumors reported in literatures of recent years in-country have peen reviewed.