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@# Objective:To explore the prognostic significance of thrombospondin 2 (THBS2) expression and its effects on the proliferation and migration of pancreatic cancerASPC-1 cells for patients with pancreatic cancer, and to investigate its possible molecular mechanism. Methods: The expression of THBS2 in pancreatic cancer tissues and its effects on overall survival rate in patients were analyzed by online database. THBS2 expression in pancreatic cancerASPC-1 cells was detected by Western Blotting; RNAinterference was used to knockdown the expression of THBS2 inASPC-1 cells, and then the effects of THBS2 knockdown on cell proliferation and migration were detected by MTT and Transwell assays, while its effects on protein expression levels (MMP, E-cadherin,AKT and PI3K) were detected by Wb. Results: Expression of THBS2 in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues (P<0.01), and the high expression of THBS2 could lead to the decrease of overall survival rate in pancreatic cancer patients. The expression of THBS2 in pancreatic cancer cell lines was significantly up-regulated; however, after interference on the expression of THBS2, the proliferation (P<0.01) and migration ability (P<0.01) of ASPC-1 cells were significantly decreased, and the expression of AKT and PI3K in cells was significantly down-regulated (P<0.01). Conclusion: THBS2 is highly expressed in pancreatic cancer tissues and cells, and is negatively correlated with the prognosis of patients. The mechanism is possibly related with the proliferation and migration of ASPC-1 cells that regulated byAKT/PI3K signaling pathway.
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ABSTRACT:Objective To explore the effects of miRNA-1246 (miR-1246)on cell proliferation,invasion and migration in human cervical squamous cell carcinoma (CSCC)cell line SiHa.Methods SiHa cells were assigned into 3 groups:miR-1246 analog group,miR-1246 antagonist group and control group.Transfection efficiency was determined.The MTT assay,transwell assay and wound healing assay were performed respectively to evaluate the proliferation,invasion and migration abilities of SiHa cells.Western blot was carried out to detect the expression of thrombospondin-2 (THBS2)before and after transfection.A THBS2 3’-UTR-containing dual luciferase plasmid was synthesized and co-transfected with miR-1246 into SiHa cells to observe the luciferase enzyme activity.Results MTT assay,transwell assay and wound healing assay revealed that the abilities of proliferation,migration and invasion were significantly enhanced (P<0.01)in SiHa cells transfected with miR-1246 analog,but suppressed in SiHa cells transfected with miR-1246 antagonist.Western blot showed that SiHa cells transfected with miR-1246 analog had significantly decreased THBS2 expression (gray value = 6 .2 8 ± 1 0 .2 2 , P=0 .0 1 3 ) while those transfected with miR-1246 antagonist had significantly increased THBS2 expression (gray value = 12.90±19.81, P=0.037).After co-transfected with miR-1246 and THBS2 3’-UTR-containing plasmid,SiHa cells exhibited a decreased level of luciferase enzyme expression.Conclusion miR-1246 promoted the proliferation,invasion and migration of CSCC SiHa cell, and it might promote CSCC tumorigenesis and progression by suppressing the expression of its target gene THBS2 .
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Objective To explore the expression of THBS 2 and its clinical significance in gastric cancer . Methods The mRNA and protein expression levels of THBS2 were assessed in 14 paired of gastric cancer specimen and corre-sponding normal mucosa using quantitative real-time PCR and Western blot analysis. Immunohistochemistry of TH-BS2 on a population-based tissue microarray consisting of 129 gastric cancer cases was used for evaluating the TH-BS2 prognostic significance. Kaplan-Meier method and Cox′s proportional hazards model were used in survival anal-ysis. Results Both mRNA and protein expression of THBS2 in 12 gastric cancer tissues were remarkably lower than the corresponding normal tissues among total 14 pair tissues. Consistent with the results of our Western blot, THBS2 expression was significantly inhibited in gastric cancer tissues as that in the normal controls in TMA ( P=0. 031) . Overexpression of THBS2 had a significant correlation with favorable prognosis of gastric cancer patients ( P=0. 002 ) and decreasing THBS2 expression was associated with the poor histological grade of gastric cancer his-tological grade ( P=0. 005 ) . Conclusion This study suggests THBS2 is down-regulation in gastric cancer tissue versus normal gastric tissue, and it may play a critical role in gastric cancer carcinogenesis and may be a potential prognosis predictor of gastric cancer.