ABSTRACT
Inflammation is a reaction of a living vascularised tissue to an injury. Conventional or synthetic drugs used in the treatment of inflammatory diseases are inadequate, it sometimes have serious side effects. So, number of herbal medicines is recommended for the treatment of inflammation that has no side effects. The present study is aimed to evaluate the anti inflammatory activity of Talinum fruticosum L on formalin induced paw edema in rats as for controlling inflammatory disorders. The objectives of the present study are to carry out phytochemical screening of selected plant drug , to prepare an aqueous extract from Talinum fruticosum L and to screen the in vivo anti inflammatory effect of Talinum fruticosum L. For phytochemical screening, the secondary metabolites like alkaloid, flavonoid, tannin, saponin, quinine were tested using qualitative spot tests. For anti inflammatory activity, wistar albino rats were used and divided into 6 groups and treated accordingly:Normal control, formalin induced group (0.1ml/kg bw), formalin+ Talinum fruticosum L (100mg/kg bw), formalin+ Talinum fruticosum L (200mg/kg bw), formalin+ Talinum fruticosum L (300mg/kg bw), and plant treated (300mg/kg bw).After the experimental period of 15 days, the blood and tissue samples were collected and biochemical parameter and histpathological studies were carried out.The phytochemical screening suggests the standardization, identity, purity and of presence of phytochemicals like saponins, tannins, flavonoids, terpenoids and cardiac glycosides. Oral administration of formalin to the experimental animals produced reduction in the levels of SOD,GSH,GPX ,GR, serum protein and total RBC and Hb. The animals pretreated with Talinum fruticosum L extract at dose levels of 100,200,300mg/kg bw were significantly increased the levels of the above parameters. A significant increase in the length of the paw thickness, in the level of serum enzymes (SGOT, SGPT, ALP, CK) and Lipid peroxide (LPO), in the level of hydroxy proline, hexosamine and leucocytes was noted in the rats induced with formalin, while these levels were normalized by pretreatment with Talinum fruticosum L extract. The histopathological studies of edematous sections of formalin induced rat paw showed loss of cartilage, osteoblast hyperplasia. Talinum fruticosum L treated formalin induced rats showed moderate reduction in the cartilage and osteoblast. From the present observation, it is evidenced that Talinum fruticosum L would be an effective drug for the treatment of inflammatory reactions.