Establishment of an in vitro fluorescence spectrophotometry based on end-product malondialdehyde for evaluating scavenging ability on hydroxyl radicals / 中国药理学通报

Aim To establish an in vitro fluorescence spectrophotometry based on the end-product malondialdehyde(MDA)for evaluating hydroxyl radical-scavenging ability, and compare the advantages and disadvantages of fluorescence and visible methods.Methods The reaction time, temperature, and the concentration of key reactant deoxyribose were investigated and optimized respectively.Under different solvent conditions, sensitivity and the measurement window of two methods were compared.The hydroxyl radical scavenging ability of tanshinone I was determined by these two methods.Results The optimal temperature and time was 37 °C and 60 min, and the concentration of deoxyribose was 2.8 mmol·L-1.The limit of detection for the fluorescence method(4.49 nmol·L-1)was much lower than that of the visible spectrophotometry(39.15 nmol·L-1).The ratio of model/control(the measurement window)of the fluorescence method was much larger than that of visible spectrophotometry in both the aqueous system and the organic system(containing DMSO).Within the concentrations of 62.5 mg·L-1-1 000 mg·L-1, tanshinone I showed scavenging ability on hydroxyl radicals in a concentration-dependent manner using the fluorescence method, but the visible method could not.Conclusions In contrast to visible method, fluorescence method has the advantages of higher sensitivity and stronger anti-interference ability to the color of test substance and the specificity of solvents.By virtue of large measurement window, it can be applied to evaluating the effect of fat-soluble test substances.

Molecular Mechanism of Salviae Miltiorrhizae Radix et Rhizoma against Bladder Cancer： Based on Network Pharmacology and In Vitro Experiment / 中国实验方剂学杂志

ObjectiveTo predict the potential targets and possible related signaling pathways of Salviae Miltiorrhizae Radix et Rhizoma against bladder cancer （BC） based on network pharmacology and verify the potential molecular mechanism through in vitro cell experiment. MethodActive components of Salviae Miltiorrhizae Radix et Rhizoma were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP） and BC-related targets were searched from GeneCards and Online Mendelian Inheritance in Man （OMIM）. Via Venny2.1， the potential targets of Salviae Miltiorrhizae Radix et Rhizoma against BC were screened out and the Venn diagram was plotted. Protein-protein interaction （PPI） network was constructed by STRING， followed by Gene Ontology （GO） term enrichment and Kyoto Encyclopedia of Genes and Gnomes （KEGG） pathway enrichment with DAVID. Cell Counting Kit-8 （CCK-8） assay was employed to detect the inhibitory effect of tanshinone ⅡA （Tan ⅡA）， cryptotanshinone （CPT）， and luteolin （LUT） at different concentration （0， 1， 2， 4， 8， 16， 32 μmol·L-1） on the proliferation of BC T24 and 5637 cells， propidium iodide （PI） staining to analyze the apoptosis of 5637 cells induced by Tan ⅡA， CPT， and LUT （0， 4， 8 μmol·L-1）， and Western blotting to detect the regulatory effect of Tan ⅡA （0， 4， 8， 16 μmol·L-1） on the expression of key target proteins. ResultA total of 65 active components and 39 anti-BC targets of Salviae Miltiorrhizae Radix et Rhizoma were screened out. The anti-BC targets were mainly involved in the KEGG pathways of neuron-ligand-receptor interaction， phosphatidylinositol 3-kinases （PI3K）/protein kinase B （Akt） signaling pathway， epidermal growth factor receptor （EGFR） tyrosine kinase inhibitor resistance， and hypoxia inducible factor （HIF）-1 signaling pathway. As for the CCK-8 assay， compared with the blank group， Tan ⅡA， CPT， and LUT significantly inhibited the proliferation of T24 and 5637 cells， particularly the 5637 cells. The half maximal inhibitory concentration （IC50） of Tan ⅡA on 5637 cells was significantly lower than that of CPT and LUT. Moreover， compared with the blank group， Tan ⅡA， CPT， and LUT all induced the apoptosis of 5637 cells， and the effect followed the order of Tan ⅡA>CPT>LUT （P<0.05）. Western blot showed that Tan ⅡA significantly reduced the expression of EGFR， p-PI3K， and p-Akt in 5637 cells in a concentration-dependent manner compared with the blank group （P<0.05）. ConclusionSalviae Miltiorrhizae Radix et Rhizoma exerts therapeutic effect on BC through multiple components， multiple targets， and multiple pathways. The mechanism is the likelihood that it down-regulates the expression of EGFR， p-PI3K， and p-Akt proteins， thus further inhibits cell proliferation， and induces apoptosis.

Efficacy and safety of Di-Tan Decoction for treating post-stroke neurological disorders: a systematic review and Meta-analysis of randomized clinical trials / 中国天然药物

The management of post-stroke complications plays an important role in the quality of life. Di-Tan Decoction (DTD; ) is a widely used traditional Chinese medicine. This study incorporated systematic review and meta-analysis to evaluate the efficacy of DTD in post-stroke neurological disorders. Randomized clinical trials (RCTs) were searched from English, Chinese and Korean electronic medical databases, by including the keywords "Di-Tan Tang", "Di-Tan Decoction", "Scour Phlegm Decoction", "stroke", and "RCT. Each RCT included control (placebo, conventional therapy, or Western medicine) and experimental (DTD treatment) groups. For patients inflicted with stroke for 1-6 weeks, the outcomes of post-stroke neurological disorders were measured by scales for post-stroke symptoms and were classified as "completely healed", "markedly effective", "effective" and "ineffective". Totally, 11 RCTs (n = 490 controls and n = 502 DTD subjects) were selected from 210 articles identified in the initial search. A meta-analysis of evaluation criteria in post-stroke symptoms revealed that the overall odds ratio (ORs) for alleviating post-stroke neurological disorders were 0.30-fold lower (95% CI = 0.21-0.43) in the DTD group than the control (Western medicine) group (P < 0.000 01). Moreover, regardless of the type of stroke diagnostic scale applied (including NFA, HDS, and NIHSS), the overall post-stroke symptoms determined were less severe in the DTD group (n = 219) than the control group (n = 217). No adverse effects of DTD were observed in the 11 RCTs reviewed. All 11 studies used an appropriate method for randomization of subjects to evaluate the risk of bias (ROB), and 7 studies included allocation concealment as well as blinding of patients and practitioners. High-risk ROB was included in 6 RCTs. No significant publication bias was derived from the funnel plot. Our results indicate that the administration of DTD alone, and DTD in combination with Western medicine, exert greater efficacy for post-stroke complication therapy, than Western medicine administered alone. More rigorous and regulated studies are required to confirm the therapeutic efficacy of DTD for post-stroke neurological disorders. disorders.

The clinical research on the efficacy of Tanreiqing injection combined with routine western medicine in treating chronic obstructive pulmonary disease at acute and aggravating period with syndrome of phlegm-heat obstructing lung / 国际中医中药杂志

Objective@#To evaluate effect of Tanreqing injection combined conventional western medicine therapy on acute exacerbation of chronic obstructive pulmonary disease (AECOPD) of Phlegm heat obstructing Lung.@*Methods@#A total of 112 patients with AECOPD were randomly divided into two groups with digital table method from February 2015 to February 2018, 56 cases in each group. The control group was treated byconventional western medicine therapy, and treatment group was treated by Tanreqing injection based on control group. Both groups' treatment lasted for 2 weeks. The modified version of British Medical Reseach Council respiratory questionnaire (mMRC) was used for severity evaluation in breath difficulty. The COPD assessment test (CAT) was used for the assessment of symptoms, activity ability, psychological, social impact and sleep. The FEV1% and FEV1/FVC were recorded. The SaO2, PaO2 and PaCO2 were recorded. The phlegm heat obstructing lung symptom scores were detected. The adverse reactions were recorded.@*Results@#The total effective rate of treatment group was 94.4% (51/54), and the control group was 77.3% (41/53). There was statistically significant difference between two groups (χ2=5.138, P=0.023). After treatment, the mMRC and CAT scores in the treatment group were significantly lower than those in the control group (t value were 17.233, 17.111, respectively, all Ps<0.01); the cough, wheezing, shortness of breath, fever, dry mouth, constipation scores were significantly lower than the control group (t value were 27.717, 29.387, 27.227, 30.268, 28.430, 29.738, respectively, all Ps<0.01). After treatment, the FEV1% (52.34% ± 5.79% vs. 46.98% ± 5.72%, t=4.817), FEV1/FVC (61.36 ± 6.52 vs. 56.93 ± 5.94, t=3.675) in the treatment group were significantly higher than the control group (P<0.01); SaO2 (90.11% ± 9.53% vs. 83.56% ± 8.84%, t=3.684); the PaO2 (91.22 ± 9.79 mmHg vs. 85.18 ± 8.80 mmHg, t=3.354) significantly higher than the control group (P<0.01), and PaCO2 (44.75 ± 4.94 mmHg vs. 51.05 ± 5.46 mmHg, t=6.261) significantly lower than the control group (P<0.01). The incidence of adverse reactions in the treatment group was 11.1% (6/54), and the control group was 7.5% (4/53). There was no statistically significant difference between two groups (χ2=0.091, P=0.763).@*Conclusions@#Tanreiqing injection combined with conventional western medicine therapy can take effectsimprove levels of SaO2 and PaO2, reduce PaCO2 level of the AECOPD patients.

Study on the optimal concentration of Tanreqing injection based on the mathematical model of "net benefit-concentration median" / 国际中医中药杂志

Objective To explore the optimal concentration of Tanreqing injection for acute upper respiratory tract infections. Methods A retrospective analysis of 531 children with acute upper respiratory tract infection treated with Tanreqing injection was conducted. These children were divided into different groups according to concentration of Tanreqing injection (i.e., the relationship between liquid and solvent, or the dilution ratio of solvent to liquid). Assume that X was the dilution ratio of solvent to liquid and the group was divided by an additional increment of 2.5 dilution units. All the children were divided into six groups as follows, groups of A (1:10≤X<1:12.5), B (1:12.5≤X<1:15), C (1:15≤X<1:17.5), D (1:17.5≤X<1:20), E (1:20≤X<1:22.5) and F (1:22.5≤X<1:25). The clinical effective rate and incidence of adverse reactions in each group were used as clinical indicators related to concentration of Tanreqing injection. Origin 8 was used to establish the relationship between the two indicators and the median of the concentration, and to seek a net benefit index that can combine the two indicators together. Finally, the relationship between the net benefit indicator and the median of the concentration were established, and the optimal concentration was found by judging and picking up the maximum of the net benefit indicator. Results The relationship between the clinical effective rate and the concentration median were: Y=-0.3+31.58X-203.33X2 (R2=0.988 46), and relationship between the incidence of adverse reactions and the concentration median were: Y=-0.27+10.24X-67.85X2 (R2=0.982 28), and relationship between the net benefit indicator and the concentration median were: ΔY=-0.03+21.34X-135.48X2. The peak value of the net benefit indicator was 0.81, and the corresponding median of the concentration was 0.078 8, and the optimal dilution ratio of liquid to solvent was 1:11.6-1:14.1. Conclusions This article explored the optimum concentration range of Tanreqing injection and gave tips for clinical safety and effective drug use.

Study on the optimal concentration of Tanreqing injection based on the mathematical model of "net benefit-concentration median" / 国际中医中药杂志

Objective@#To explore the optimal concentration of Tanreqing injection for acute upper respiratory tract infections.@*Methods@#A retrospective analysis of 531 children with acute upper respiratory tract infection treated with Tanreqing injection was conducted. These children were divided into different groups according to concentration of Tanreqing injection (i.e., the relationship between liquid and solvent, or the dilution ratio of solvent to liquid). Assume that X was the dilution ratio of solvent to liquid and the group was divided by an additional increment of 2.5 dilution units. All the children were divided into six groups as follows, groups of A (1:10≤X<1:12.5), B (1:12.5≤X<1:15), C (1:15≤X<1:17.5), D (1:17.5≤X<1:20), E (1:20≤X<1:22.5) and F (1:22.5≤X<1:25). The clinical effective rate and incidence of adverse reactions in each group were used as clinical indicators related to concentration of Tanreqing injection. Origin 8 was used to establish the relationship between the two indicators and the median of the concentration, and to seek a net benefit index that can combine the two indicators together. Finally, the relationship between the net benefit indicator and the median of the concentration were established, and the optimal concentration was found by judging and picking up the maximum of the net benefit indicator.@*Results@#The relationship between the clinical effective rate and the concentration median were: Y=-0.3+31.58X-203.33X2 (R2=0.988 46), and relationship between the incidence of adverse reactions and the concentration median were: Y=-0.27+10.24X-67.85X2 (R2=0.982 28), and relationship between the net benefit indicator and the concentration median were: ΔY=-0.03+21.34X-135.48X2. The peak value of the net benefit indicator was 0.81, and the corresponding median of the concentration was 0.078 8, and the optimal dilution ratio of liquid to solvent was 1:11.6-1:14.1.@*Conclusions@#This article explored the optimum concentration range of Tanreqing injection and gave tips for clinical safety and effective drug use.

Summarization of the Experience of Luteinized Unruptured Follicle Syndrome's Treatment by Professor TAN Yong / 浙江中医药大学学报

[Objective] This paper introduces the experience of Professor TAN Yong's treatment of the luteinized unruptured follicle syndrome(LUFS) with a view to provide references for the related study.[Methods]Through studying with Professor TAN, collecting and analyzing clinical case data, researching editorial book and articles. From the etiology, pathogenesis and drug selection of the LUFS, the clinical experience of the teacher was discussed, Professor TAN's academic characteristics on the treatment of LUFS were summarized, and a medical case was provided to support the idea.[Results]Professor TAN believes that the pathogenesis of this disease is mostly due to deficiency of the kidney Yin, the weak Yang qi, Yin and Yang conversion is unfavorable during intermenstrual period, combined with pathological factors such as liver depression, blood stasis, and phlegm dampness. Unsmooth running of Qi and blood, so that the follicular can not be discharged smoothly, the disease occurs. In the treatment of combination of Traditional Chinese Medicine(TCM) and western Medicine, in the intermenstrual period attach importance to the function of the heart and brain, to tonifying kidney and blood circulation as a method, the use of "Tonifying Kidney Stimulating Ovulation" medicine treatment, combined with western medicine, in the clinically achieved good results. The medical case was based on the "Tonifying kidney Stimulating Ovulation" medicine as the basis for the successful pregnancy after the treatment, which fully proved the above mentioned dialectical thinking and treatment experience. [Conclusion]It is worthwhile to learn and promote the treatment of LUFS with tonifying kidney and blood circulation therapy.

Clinical effect of Huayi decoction in the treatment of recurrent endometriosis / 中国基层医药

Objective To study the effect of Huayi decoction in the treatment of recurrent endometriosis ,and its influence on Th1/Th2 balance.Methods 92 patients with recurrent endometriosis were selected as the research subjects.According to the digital table method ,the patients were randomly divided into the observation group and the control group,46 cases in each group.The control group was given mifepristone ,every time 12.5mg,1 time/day, continuously treated for 3 months.The observation group was treated with Huayi decoction for 3 months.The serum carbohydrate antigen 125(CA125),interferon gamma(IFN-γ),interleukin 4(IL-4) were measured before and after treatment.Results After treatment,the effective rate of the control group was 78.3％,which was significantly lower than 91.3％ of the observation group (χ2 =5.16,P<0.05).Before and after treatment,the serum CA125 levels of the control group were (103.62 ±27.45) IU/mL,(55.21 ±19.23) IU/mL,respectively.Before and after treatment,the serum CA125 levels of the observation group were (106.85 ±22.74)IU/mL,(31.47 ±12.94)IU/mL, respectively.After treatment,the serum CA125 levels were significantly lower in the two groups (t=10.963,14.206, all P<0.01),and the serum CA125 level in the observation group was significantly lower than that in the control group (t=5.038,P <0.05).The IL -4,IFN -γ,IL -4/IFN -γof the control group before treatment were (22.61 ±6.72) pg/mL,(51.77 ±8.09) pg/mL and (0.41 ±0.11),respectively,which after treatment were (15.16 ±5.04)pg/mL,(60.39 ±9.14)pg/mL and (0.22 ±0.07),respectively.The IL-4,IFN-γ,IL-4/IFN-γof the observation group before treatment were (23.01 ±6.71) pg/mL,(51.16 ±7.63) pg/mL and (0.42 ± 0.15),respectively,which after treatment were (9.03 ±3.37)pg/mL,(69.91 ±8.24)pg/mL and (0.12 ±0.05), respectively.After treatment,the serum IL-4 levels of the two groups were significantly lower (t=6.0143,8.770,all P<0.01),and the decrease of IL -4 level of the observation group was more significant (t=3.448,P<0.05).After treatment,the serum IFN-γlevels were significantly higher in the two groups (t=2.014,2.998,all P<0.05),and the increase of IFN-γlevel of the observation group was more significant (t=2.106,P<0.05).After treatment,the serum IL-4/IFN-γof the two groups were significantly lower (t=5.182,9.062,all P<0.01),and the IL-4/IFN-γdecreased more significantly in the observation group (t=5.824,P<0.05).Conclusion Huayi decoction can significantly improve the postoperative curative effect and improve the imbalance of Th 1/Th2.

Cuando la baciloscopía positiva no es tuberculosis. A propósito de un caso y breve review de la literatura / When smear positive is not tuberculosis. About a case and brief review of the literature

El método gold standar para el diagnóstico de la tuberculosis (TB) es el cultivo en medio sólido de Lowenstein-Jensen. Dado el alto costo y los requerimientos de infraestructura y personal entrenado que exige este método, tanto el programa nacional, como las recomendaciones de la Organización Mundial de la Salud (OMS), establecen que una baciloscopía positiva (BAAR +) confirma el diagnóstico de TB. Si bien el cultivo amplía la sensibilidad diagnóstica, tipifica los bacilos, y permite realizar pruebas de sensibilidad, no es un requisito operativo para la definición de confirmación bacteriológica de TB. En Argentina, el 1% de las BAAR (+) corresponden a otras etiologías3. Presentamos un caso comprendido en ese 1%, en el cual mostramos los problemas a los que se enfrenta un paciente (fundamentalmente demora diagnóstica y exposición innecesaria a drogas potencialmente tóxicas) cuando tiene un diagnóstico de TB de acuerdo con los stándares nacionales e internacionales y sin embargo no es TB.

The method gold standard for the diagnosis of tuberculosis (TB) is the culture in Lowenstein-Jensen’s solid way. In view of the high cost and the requirements of infrastructure and trained personnel that demands this method, both the national program, and the recommendations of the World Health Organization (WHO), they establish that a smear-positive confirms TB’s diagnosis. In addition, the culture increases the diagnostic sensitivity, typifies the bacillus and allows the sensitivity tests to be performed, but it is not an operative requirement for the definition of TB’s bacteriological confirmation. In Argentina, 1% of the BAAR (+) are due to other etiologies3. We present a case included in that 1%, in which we show the problems that a patient faces (basically diagnostic delay and unnecessary exposure to potentially toxic drugs) when it has TB’s diagnosis of agreement to the national and international standards and nevertheless it is not TB.

Experience of TAN Jin-chuan in Treatment of Idiopathic Membranous Nephropathy / 中国中医药信息杂志

Idiopathic membranous nephropathy (IMN) is one of the main causes of nephrotic syndrome in adults, and is a kind of glomerular capillary loops epithelial side immune complex deposition of glomerular disease. At present, Western medicine treatment mainly uses hormone combined with immunosuppressive agent, but the adverse reactions are bigger. According to many years clinical experience, Professor TAN Jin-chuan starts from the spleen and kidney deficiency and blood stagnation in kidney meridian, and uses modified Yishen Tongluo Prescription to support right qi and regulate spleen and kidney at the same time. She is good at use of insect medicine to seize the meridians, and focuses on comprehensive care, which provides positive reference value for TCM treatment of IMN.

HAN Xinmin's Experience in Treating Tic Disorders from View of Wind-phlegm / 浙江中医药大学学报

Objective]The paper summarizes the academic thoughts and clinical experience of Professor HAN Xinmin for treating Tic Disorders from view of wind-phlegm. [Methods] By learning from Professor HAN Xinmin, recording the relevant cases and analyzing typical cases, to sum up the knowledge of etiology and pathogenesis, treatment characteristics, as well as list one proved case. [Results]Professor HAN Xinmin holds that this disease origins from liver, involving lung, spleen and kidney. The key pathogenesis is the internal stir of wind and phlegm. Treatment follows the principles of dispelling pathogenic wind and eliminating phlegm, along with suppressing hyperactivity liver qi, dispersing lung qi, regulating spleen and tonifying kidney. The self-ordained Chinese herbal medicine prescription has achieved a satisfactory clinical curative effect. [Conclusion]Therapies of Professor HAN Xinmin on treating Tic Disorders have significant effect, which have the value of popularization and application.

Experience of TAN Qing-gang in Differentiation and Treatment for Postoperative Breast Cancer / 中国中医药信息杂志

Breast cancer is one of the most common malignant tumors in women, which affects female physical and mental health seriously, and even has a life-threatening effect. TCM plays a more and more important role in the treatment of breast cancer today. Many veteran TCM doctors hold unique opinions about it, especially for postoperative complications. Doctor TAN Qing-gang is one of the most famous and experienced TCM doctors in the field of TCM in Enshi, Hubei Province. He is expert in the diagnosis and treatment on miscellaneous diseases of TCM. This article summarized the experience of Doctor TAN in treatment for postoperative breast cancer.

Pharmacodynamics Research on Antitussive, Expectorant, Antiasthmatic and Antiinflammatory Effect of Ju-Hong Tan-Ke Liquid / 世界科学技术-中医药现代化

This study was aimed to investigate the antitussive,expectorant,antiasthmatic and anti-inflammatory effect of Ju-Hong Tan-Ke (JHTK) liquid.The methods of cough induced by ammonia in mice and cough induced by citric acid in guinea pigs were used to observe the antitussive effects.The mouse phenolsulfonphthalein excretion method,rat capillary expectoration method and the rabbit tracheal cilia movement influence experiment were used to observe the expectorant effect.An experiment of allergen induced rats' asthma was used to observe the antiasthmatic effects.The anti-inflammatory effect was observed by mouse peritoneal capillary permeability test and xylene induced ear swelling in mice.The results showed that JHTK liquid could inhibit cough induced by ammonia in mice,decrease the frequency of cough induced by citric acid in guinea pig and prolong the latent period of cough.The medicine could also obviously increase phenol red output of trachea in mice,promote expectoration in rats,accelerate the movement of cilium of tracheal transparently and prolong the latent period of asthma induced by allergens in rats,inhibit capillary permeability and lighten ear edema in mouse model.It was concluded that JHTK liquid could inhibit the reflex cough caused by hypersensitivity of airway sensor.It is peripheral antitussive rather than central antitussive,which has significant antitussive,expectorant,antiasthmatic and anti-inflammatory effects.Its efficacy is superior or equivalent to positive chemical control medicine.

Curative Effect Observation of Ju-Hong Tan-Ke Liquid in Treatment of Patients with Anemofrigid (or Mixing with Damp) Cough / 世界科学技术-中医药现代化

This study was aimed to observe the clinical efficacy of Ju-Hong Tan-Ke (JHTK) liquid in the treatment of patients with anemofrigid (or mixing with damp) cough.A total of 70 patients with anemofrigid (or mixing with damp) cough admitted to Chinese medicine clinic in our hospital were selected and randomly divided into the treatment group (n =50) and the control group (n =20).Patients in the treatment group were treated with 2 JHTK liquid (Guangzhou Xiangxue Pharmaceutical Co.,Ltd.,20 ml),3 times a day.Two She-Dan Chen-Pi (SDCP) liquid (20 ml) was used in the control group,3 times a day.The treatment course was 1 week.Differences of the clinical efficacy of cough and expectoration symptoms between two groups after treatment were compared.The effect of JHTK liquid on different degrees of cough and expectoration was also compared.The results showed that the total effective rate of cough in the treatment group was 92.00％,which was obviously higher than 70.00％ of the control group with statistical difference (P＜ 0.05).The total effective rate of expectoration symptoms in the treatment group was 94.00％,which was obviously higher than 75.00％ of the control group with statistical difference (P＜0.05).The curative effects of JHTK liquid in the treatment of mild,moderate and severe cough were 90.00％,93.75％,and 87.50％,respectively.The curative effects of JHTK liquid on mild,moderate and severe expectoration were 100％,96.77％,and 80.00％,respectively.It was concluded that JHTK liquid can effectively improve cough and expectoration symptoms of patients with anemofrigid (or mixing with damp) cough,especially the clinical curative effect of mild and moderate cough with expectoration was obviously and the effect of severe cough and expectoration was excellent.Its curative effect was superior to SDCP liquid,which is worthy of clinical application.

Optimization of preparation procedures of tanshinone-ⅡA /β-cyclodextrin inclusion complex and dissolution study of tanshinone-ⅡA in vitro / 中国中药杂志

In this paper, tanshinone-ⅡA (Tan-IIA)/β-cyclodextrin (β-CD) inclusion complexes were prepared by saturated aqueous solution method. Based on the single factor experiment, Box-Benhnken design and response surface method were utilized to optimize the preparation procedures of tanshinone-ⅡA/β-cyclodextrin inclusion complexes. The ratio of β-CD to Tan-ⅡA, experimental temperature and time were defined as independent variables, while the yield of the inclusion complexes, encapsulation efficiency and the generalized "normalized value" were set as the response value. In addition, the inclusion complexes were characterized by infrared spectroscopy (IR) and nuclear magnetic resonance (NMR). The results showed that optimum preparation conditions for Tan-ⅡA/β-CD inclusion complex were as follows: Tan-ⅡA/β-CD ratio of 1:7, the temperature of 48 ℃ and the time of 3 h. Under the optimized conditions, the encapsulation efficiency of Tan-ⅡA/β-CD inclusion complex was 84.75%. The Tan-IIA and β-CD inclusion complex can significantly improve the dissolution of Tan-ⅡA.

Effect of xiao-tan-jie-yu prescription on sleep quality of soldiers acute exposure to high altitude / 解放军医学杂志

Objective To observe the efficacy of Xiao-tan-jie-yu prescription (XTJYF) on sleep quality of the soldiers who acutely exposed to western area of high altitude. Methods In this prospective, completely randomized, parallel, placebocontrolled study, 550 soldiers acutely exposed to western area of high altitude were investigated by the Pittsburgh Sleep Quality Index (PSQI), 100 soldiers with sleep disorder were selected and divided into two groups (50 each): treatment group received TCM XTJYF therapy and control group was treated with placebo. After 2 weeks' treatment, PSQI total score and respective factor scores before and after treatment were assessed, and clinical therapeutic efficacy and adverse reactions were observed. Results The PSQI total score and respective factor scores of these soldiers were significantly higher than those of normal adults, but significantly lower than those of insomnia patients, while their sleep disorder factor score was significantly higher compared with insomnia patients. XTJYF reduced the total score and some factor scores (subjective sleep quality, time for initiating sleep, total sleep time, and sleep efficiency) for PSQI in the soldiers with sleep disorder, and the overall response rate was 91.49% which is higher than those in the placebo control group (P<0.05 or 0.01), without toxic side effects. Conclusions The sleep quality of soldiers who acutely exposed to western area of high altitude in China is not high, and XTJYF may safely and effectively improve the sleep quality.

Algunas formas alternativas de ejercicio, una opción a considerar en el tratamiento de personas con diabetes mellitus / Some alternative forms of exercising as a choice to be considered in the treatment of individuals with diabetes mellitus

Introducción: Muchas personas, como parte del tratamiento de su diabetes mellitus, prefieren hacer ejercicios alternativos, incluyendo actividades mente-cuerpo empleados en la Medicina Tradicional China.Objetivo: describir algunas formas alternativas de ejercicios utilizados por la Medicina Tradicional China como una opción a considerar en el tratamiento de personas con diabetes mellitus.Desarrollo: se encontraron efectos similares a los reportados con el uso de la actividad física convencional en personas con diabetes mellitus, que al parecer pueden ser obtenidos con los ejercicios utilizados en la Medicina Tradicional China, como es el caso de la práctica del Tai chi chuan, Qigong y Wai tan kung. Ellos constituyen programas de ejercicios útiles, bien estructurados y con miles de años de práctica cotidiana, que, unidos al tratamiento convencional de la diabetes mellitus, pueden facilitar una buena evolución y una mayor calidad de vida de estas personas, pues aportan efectos positivos sobre el control metabólico, la densidad ósea, la función física general y cardiopulmonar en particular, y sobre el estado psicológico y la función inmune del paciente.Conclusiones: los efectos favorables obtenidos durante la realización de formas alternativas de ejercicio físico utilizados por la Medicina Tradicional China en personas con diabetes mellitus, hacen de su práctica una opción a considerar para el tratamiento de esta importante entidad nosológica(AU)

Introduction: As part of the treatment of diabetes mellitus, many people choose to do alternative exercises including mind-body activities used in the Chinese traditional medicine.Objective: to describe some alternative forms of exercising used by Chinese traditional medicine as a choice to be considered in the treatment of diabetes mellitus people.Development: similar effects to those reported with the use of conventional physical activity in diabetic persons were found; it seems that they may be reached with the Chinese traditional medicine exercising as is the case of practicing Tai chi chuan, Qigong and Wai tan kung. These are well-structured programs of useful exercises that have been practiced during thousand of years and that, together with the conventional therapy for diabetes mellitus, could facilitate good progress and higher quality of life in this people. They provide positive effects in terms of metabolic control, bone density, general physical function and particularly, cardiopulmonary function, psychological status and immunological condition of the patient.Conclusions: the favorable impact from the practice of alternative forms of physical exercises in Chinese traditional medicine by diabetes mellitus patients makes practice a choice to be considered in treating this important entity(AU)

Effect ofTan-Re-QingInjection on Perioperative Period of TNF-α, IL-6, IL-8 and IL-10 in Lung Cancer Patients Complicated with COPD / 世界科学技术-中医药现代化

This study was aimed to explore the possibility of reducing postoperative inflammatory response and lung injury degree by observing the effect ofTan-Re-Qing(TRQ) Injection on perioperative period of lung cancer patients complicated with chronic obstructive pulmonary disease (COPD). A total of 39 lung cancer cases complicated with COPD (phlegm-heat obstructing the lung) were randomly divided into the tested group of 18 cases and the control group of 21 cases. All patients underwent small incision surgery with video-assisted thoracoscopic surgery (VATS). The control group was treated with routine western medicine. TRQ injection was added in the tested group. On the preoperative 1D (T1), 1D after operation (T2), 3D after operation (T3), 7D after operation (T4), serum samples were collected for the determination of serum concentrations of TNF-α, IL-6, IL-8 and IL-10. At T1 and T2 time, there were no significant differences on levels of TNF-α, IL-6, IL-8 and IL-10 between two groups of patients (P > 0.05). At T3 time, levels of TNF-α, IL-6, IL-8 of the tested group were lower than that of the control group with statistical significance (P < 0.05). The level of IL-10 in the tested group was higher than that of the control group with statistical significance (P < 0.05). At T4 time, the levels of TNF-α, IL-6 and IL-8 of the tested group were lower than that of the control group with statistical significance (P < 0.05). The levels of TNF-α, IL-6 and IL-8 of both groups gradually declined at T3 and T4 time, compared with T2 time level with statistical significant (P < 0.05). It was concluded that TRQ injection reduced the releasing of inflammatory cytokines during perioperative period, increased levels of anti-inflammatory cytokine, thereby reducing the degree of inflammatory reaction and relieving lung injury for the protection of lung function of lung cancer patients complicated with COPD.

Study on Intervention of Serum 3-deoxyglucosone byBu-Shen Hua-TanFang in Rats with Diabetes Mellitus and Cognitive Impairment / 世界科学技术-中医药现代化

This paper was aimed to study the influence ofBu-Shen Hua-TanFang (BSHTF) on the learning and memory function of diabetes mellitus (DM) rats. A total of 50 3-month-old male Wistar rats were randomlydivided into the control group, model group and 3 BSHTF groups (i.e., high-, middle-, and low-dose). A total of 35 mg·kg-1 streptozotocin (STZ) was intraperitoneally injected into the model and BSHT group. The same volume of citric acid-sodium citrate buffer solution was intraperitoneally injected into the control group. The intragastric administration of BSHTF at the dose of 20, 10, and 5 g·kg-1 was given to the BSHTF groups, respectively. The behavior and content of serum 3-deoxyglucosone (3-DG) were detected among DM rats after 30 days. The results showed that the levels of serum glucose in the model group and the BSHTF groups were obviously higher than that of the control group (P < 0.01). The swimming time to platform of rats in the model group was obviously prolonged compared to the control group with significant difference from the second day on (P < 0.01). The required time in different BSHTF intervention groups was obviously decreased compared with the model group from the 4th day (P< 0.05). The level of serum 3-DG in rats of the model group was obviously higher than that of the control group (P < 0.01). The level of serum 3-DG in rats of the middle-dose BSHTF group was obviously decreased compared with the model group (P < 0.05). It was concluded that BSHTF was able to improve the cognitive impairment among DM rats by reducing the levels of serum glucose and serum 3-DG.

Study on content of eucalyptol in chao aiye and aiye tan / 中国生化药物杂志

Objective To improve the quality of aiye processed products, an eucalyptol content in commercially available aiye two processed products of chao aiye and aiye tan was investigated.Methods A capillary gas chromatography was used.The sample was prepared with n-hexane by reflux condensation.Chromatographic conditions: The separation was carried on an Ailgent DB-1 capillary column(30 mm ×0.320 mm ×0.25 μm). Inlet temperature was 200℃ and FID temperature was 250℃.The programmed column temperature was set as follows:maintained at 100℃ for 6 min and raised to 160℃ at the rate of 20℃/min followed by holding for 3min.The splitting-ratio was 5.0:1.The carried gas was nitrogen, flow rate was 1.0 mL/min.Injection volume was 1μL.Results In the given chromatographic conditions, the eucalyptol chromatographic separation had good, and the separation degree was greater than 1.5 between eucalyptol and other impurity peak.The linear range of eucalyptol was 11.4-114.0 mg/mL(r=0.999 5). Methods repeatability and recovery were good.The minimum limit of quantification was 0.5μg/mL.The results of determination of eucalyptol show that the eucalyptol content in the commercially available 11 batch of chao aiye was between 5.6-78.2 μg /g, and 12 batch of aiye tan had no eucalyptol. Conclusion The processing technology of current commercially available aiye processed products of chao aiye and aiye tan need to be improved, and the quality standard need to be improved.