ABSTRACT
Objective: To investigate the metabolism effect of tannins from Granati Pericarpium in intestinal flora for elucidating the metabolic pathway of tannins from Granati Pericarpium in the intestinal flora. Methods: Pomegranate tannins were incubated with rats intestinal flora for 4, 6, 8, 12, 24, and 48 h. The content was then detected at different times. Results: A total of 10 metabolites were detected in the rats intestinal flora by LC-MS, namely urolithin B, gallic acid, pyrogallic acid, 3-O-methylgallic acid, urolithin A, methyl-urolithin A, urolithin C, urolithin-M6, urolithin A-glucuronidation, urolithin C-glucuronidation. Conclusion: Tannins from Granati Pericarpium in intestinal flora could generate physiological activity of small molecule compounds through hydrolysis of metabolic enzymes, and these compounds may be the material basis of tannins from Granati Pericarpium and play the role of pharmacological activities in the body.