Relationship between nucleoprotein TAR DNA/RNA binding protein 43 and mouse amyotrophic lateral sclerosis / 解剖学报

Objective Transgenic mice expressing human TAR DNA/RNA binding protein 43 (hTDP-43) mutant protein in spinal cord motor neurons were constructed using HB9 promoter to establish a disease model of amyotrophic lateral sclerosis ( ALS) and explore the mechanism of ALS induced by hTDP-43 mutation. Methods HB9 promoter junction mutant hTDP-43 vector was constructed in vitro, and the positive transgenic mouse strains were prepared by prokaryotic injection and screened (There were 8-10 mutations at Q331K and M337V). Gait analysis, rotary rod fatigue test, and suspension test were used to detect locomotion ability of mice. Immunohistochemistry, immunofluorescence staining and Western blotting were used to detect hTDP-43, phosphorylated HTDP-43 ( p-hTDP-43) , Caspase-3, cleaved Caspase-3, respectively. Expression of ubiquitin, (3-tubulinIH(Tujl) , Ki67 and cyclin-dependent kinase 5 (CDK5) proteins were also detected. Results In transgenic mice expressing mutant hTDP-43 protein in spinal motor neurons, both hind limbs were atrophied to the trunk side, and motor function showed progressive decline with increasing age. hTDP-43, p-hTDP-43, Caspase-3, and cleaved Caspase-3 were observed in spinal motor neurons Caspase-3 positive staining and ubiquitin protein positive inclusion body, and in vitro isolation and culture of spinal motor neurons, it was found that hTDP-43 and ubiquitin protein co-located in choline acetyl translocation enzyme ( ChAT) positive motor neurons, accompanied by ectopic expression of CDK5. Conclusion The mutant HDP 43 protein expressed in mouse spinal cord motor neurons can promote the re-entry of differentiated mature neurons into the cell cycle, leading to the occurrence of ALS.

Personas con VIH en hospitales públicos de CABA: antirretrovirales y respuestas al tratamiento. Aún lejos de los objetivos de la OMS / People with HIV in Public Hospitals of CABA: Antiretrovirals and treatment responses. Still far from WHO ́s goals

Introducción: Se estima que en Argentina son 129 mil las personas que poseen el virus de inmunodeficiencia humana, aunque un 20% de ellas lo desconoce. El 20% de 5800 nuevos casos por año en el país corresponden a CABA. No hay datos sobre la situación de pacientes que se atienden en hospitales públicos de CABA con respecto a objetivos de la OMS. Objetivo: Analizar esquemas antirretrovirales y situación virológica e inmunológica de las personas con VIH (PcVIH) en TAR que se asisten hospitales públicos de CABA. Métodos: Estudio descriptivo y retrospectivo, bajo muestreo por conveniencia. Análisis de datos obtenidos del Sistema de Administración de Pacientes VIH (SVIH), activos a junio de 2018. Es dable aclarar que la primera parte se refiere a la fuente de información, y que sean activos a junio de 2018 se refiere a criterio de inclusión y periodo de análisis respectivamente. Variables analizadas: sexo, edad, distribución de TAR, CV y CD4 utilizando el programa SPSS 20. Resultados: 6878 PcVIH en TAR, 66,6% hombres. 79% con al menos 1 CV. De ellas, con CV indetectables (<40 copias) 62% (78% del total de PcVIH con CV). Datos de CD4 en 56% PcVIH: 65% con >500. En primera línea de TAR 88,3%: 42% IP potenciados, 45% INNTI, 13% INSTI. De CV indetectables (<40 copias), 92% en primera línea de TAR (significativo) y de las detectables, 80%. INNTI como tercera droga, significativo para CV indetectable (<40 copias). 45% de PcVIH en TAR sin CV y/o CD4. Discusión: La muestra representa la mitad de las PcV1 de CABA atendidas en el sistema público. Más de la mitad de la población estudiada se encuentra indetectable (<40 copias). INNTI siguen siendo las terceras drogas más utilizadas. Alto % de PcVIH en TAR sin CV y/o CD4. Insuficientes laboratorios (CV y CD4), baja disponibilidad de datos y lejanía del objetivo de OMS indican deficiencias del sistema de salud. Mejoras en acceso a TAR, a determinaciones de laboratorio, y en la calidad de los datos son necesarios.

Introduction: HIV infection is significant in Buenos Aires City. 20% of 5800 new cases/year in the country are from this region. There ́s no data about PLwHIV treated in public hospitals of Buenos Aires City about WHO objectivesObjetives: To know and analyze the situation PLwHIV treated in p ublic hospitals of Buenos Aires City about treatment, and virological and immunological responses.Methods: Descriptive and retrospective study. Data obtained from the Administration System of HIV Patients actives in June 2018. Variables analyzed: gender, age, ART distribution, VL and CD4 counts, with SPSS 20 ProgramResults: 6878 PLwHIV on ART, 66,6% male. 79% with at least 1 VL. Of them, 62% with undetectable VL (78% of all PLwHIV with VL). CD4 data in 56% PLwHIV: 65% >500: In 1st line of TAR 88,3%: 42% boosted IPs, 45% NNRTIs, 13% INIs. Of PLwHIV with undetectable VL, 92% in 1st line of ART (significant). NNRTIs significant for undetectable VL.Discussion:Our cohort represents 50% de los PLwHIV of Buenos Aires City. 62% currently with undetectable VL. NNRTIs still the more used 3rd antiretroviral. High % of PLwHIV on ART without VL and/or CD4 count. Not enough laboratory determinations (VLs and CD4 counts), low data ava ilability and remoteness of WHO ́s goals for 2020, show health system ́s weakness. Improvements in Access to ART and laboratory determinations and in the data availability andquality are necessary.

Repensando o ato de pesquisar - negociações, composições e afetações / Repensando el acto de investigación - negociaciones, composiciones y afectaciones / Rethinking the act of research - negotiations, compositions and affections

Resumo: Este artigo busca trazer contribuições para se pensar métodos de pesquisa a partir da Teoria Ator-rede (TAR), com autores como Latour, Despret, Favret-Saada, dentre outros - no intuito de trazer visibilidade a novas maneiras de se posicionar enquanto pesquisador. Nesta perspectiva, o pesquisador se encontra em um movimento de tradução e negociação, em um curso de ação composto por agentes diversos. Uma vez que se concebe que as ciências não estão recortadas do resto dos acontecimentos dispersos no mundo, pode então o cientista dar-se a percorrer uma infinidade de possibilidades de intervenção ao seguir as ações e seus agentes. Deste modo, o que estaria em pauta seria a produção de boas generalizações e não a comprovação de verdades previamente estabelecidas. Neste sentido, o caminho de pesquisa estaria representado pela capacidade de articulação das ciências com temas para além de suas fronteiras, compondo outras traduções, mais amplas e inclusivas daquilo que as cercam.

Resumen: Este artículo busca hacer contribuciones para pensar el método de investigación a partir de Latour y demás autores como Despret y Favret-Saada, con el propósito de traer visibilidad a nuevas maneras de posicionarse como investigador. En esta perspectiva, el investigador se encuentra en un movimiento de traducción y negociación, en un curso de acción compuesto por agentes diversos. Una vez que se concibe que las ciencias no están recortadas del resto de los acontecimientos dispersos en el mundo, puede entonces el científico darse a recorrer una infinidad de posibilidades de intervención al seguir las acciones y sus agentes. Así, lo que estaría en pauta sería la producción de buenas generalizaciones y no la comprobación de verdades previamente establecidas. En este sentido, el "interés" discutido por Latour sería representado por la capacidad de articulación de las ciencias con temas más allá de sus fronteras, componiendo otras traducciones, más amplias e inclusivas de aquello que las rodea.

Abstract: This article makes some contributions to think about the research method from Latour and other authors such as Despret and Favret-Saada, in order to bring visibility to new ways of positioning oneself as a researcher. In this sense, the researcher is in a movement of translation and negotiation, in a course of action composed of diverse agents. Once the sciences are conceived as not being out from the rest of the world's dispersed events, then the scientist can go through a myriad of possibilities of intervention by following the actions and their agents. Thus, what would be in question would be the production of good generalizations and not the proof of previously established truths. In this sense, the "interest" discussed by Latour would be represented by the capacity of articulation of the sciences with themes beyond their borders, composing other translations, broader and more inclusive of what surrounds them.

Retinal immaturity at first screening and retinopathy of prematurity: Image-based validation of 1202 eyes of premature infants to predict disease progression

Purpose: To use the extent of retinal immaturity at the first visit to predict progression to any stage and treatment-requiring retinopathy of prematurity (ROP). Methods: Retrospective, multicenter, nonrandomized, observational, clinical, validation study. In all, 601 Asian Indian preterm infants born < 2000 g and/or < 34 weeks of gestation completing ROP screening with RetCam images taken during each visit were included. A total of 1202 eyes of these infants were classified into three groups based on the retinal immaturity at the first screening visit into “mild” (Group 1), vessels reaching the posterior boundary of zone 3; “moderate” (Group 2), vessels entering zone 2 anterior; and “severe” (Group 3), vessels in zone 1 or zone 2 posterior. RetCam images at each subsequent visit were evaluated and the proportion of eyes that progressed to Type 1 or Type 2 ROP was correlated with the degree of retinal immaturity. Results: Of the 958 eyes in Group 1, 200 eyes in Group 2, and 44 eyes in Group 3, any stage ROP developed in 15% of eyes in Group 1, 46.5% of eyes in Group 2, and 100% of eyes in Group 3 (P < 0.001). Sixteen of 128 eyes (12.5%), 12 of 72 (16.6%), and 28 of 44 of eyes (63.6%) in Groups 1, 2, and 3, respectively, required treatment (P < 0.001). Conclusion: Retinal immaturity at first screening visit predicts Type 1 and Type 2 ROP. “Severe” immaturity is more likely to progress to “treatment-requiring” disease. This could be a useful tool for prognostication, counseling, and scheduling follow-up.

Impaired Nucleoporins Are Present in Sporadic Amyotrophic Lateral Sclerosis Motor Neurons that Exhibit Mislocalization of the 43-kDa TAR DNA-Binding Protein

BACKGROUND AND PURPOSE: Disruption of nucleoporins has been reported in the motor neurons of patients with sporadic amyotrophic lateral sclerosis (sALS). However, the precise changes in the morphology of nucleoporins associated with the pathology of the 43-kDa TAR DNA-binding protein (TDP-43) in the disease process remain unknown. We investigated the expression of nucleoporins that constitute the nuclear pore complex (NPC) in spinal motor neurons that exhibit sALS in relation to TDP-43 pathology, which is a reliable neuropathological hallmark of sALS. METHODS: Paraffin-embedded sections of the lumbar spinal cord were obtained for immunofluorescence analysis from seven control subjects and six sALS patients. Anti-TDP-43 antibody, anti-nucleoporin p62 (NUP62) antibody, and anti-karyopherin beta 1 (KPNB1) antibody were applied as primary antibodies, and then visualized using appropriate secondary antibodies. The sections were then examined under a fluorescence microscope. RESULTS: NUP62 and KPNB1 immunoreactivity appeared as a smooth round rim bordering the nuclear margin in normal spinal motor neurons that exhibited nuclear TDP-43 immunoreactivity. sALS spinal motor neurons with apparent TDP-43 mislocalization demonstrated irregular, disrupted nuclear staining for NUP62 or KPNB1. Some atrophic sALS spinal motor neurons with TDP-43 mislocalization presented no NUP62 immunoreactivity. CONCLUSIONS: Our findings suggest a close relationship between NPC alterations and TDP-43 pathology in the degenerative process of the motor neurons of sALS patients.

Separación de componentes con liberación del músculo transverso (TAR) para el tratamiento de grandes defectos de la pared abdominal / Component separation with transverse abdominis muscle release (TAR) for the treatment of large abdominal wall defects

Antecedentes: numerosas técnicas han sido descriptas para el tratamiento de defectos complejos de la pared abdominal. La técnica de separación de componentes con liberación del músculo transverso (TAR) permite la movilización de colgajos miofasciocutáneos y cierre de la línea media, con baja tasa de complicaciones. Objetivo: evaluar los resultados del tratamiento de eventraciones complejas con técnica TAR. Material y métodos: se incluyó una serie de pacientes en los que se realizó TAR entre marzo de 2013 y abril de 2017, con seguimiento mínimo de 6 meses. Se analizaron variables demográficas y clínicoquirúrgicas. Los resultados fueron expresados en medianas. Resultados: se realizaron 14 eventroplastias con técnica TAR. Diez (70%) correspondieron a hombres. La edad fue 60 (35-81) años y el IMC fue: 28,2 kg/m². El 70% tenía al menos dos cirugías previas. Se solicitó tomografía computarizada preoperatoria en todos los pacientes. El índice de Tanaka fue 24,5%. En 7 (50%) pacientes el riesgo quirúrgico fue ASA < III. El tamaño del defecto fue 480 (224-720) cm² y el de la malla 900 (500-1050) cm². El tiempo operatorio fue 248 (180-341) minutos, y la estadía hospitalaria, 3 (2-4) días. Dos pacientes tuvieron infección del sitio quirúrgico. El seguimiento posoperatorio fue de 20 (6-48) meses, y no se detectaron recidivas. Conclusión: la técnica TAR es un método seguro y fiable para la reparación de grandes defectos de la pared abdominal con baja morbimortalidad y recidiva, por lo que debería considerarse dentro del armamentario quirúrgico.

Background: several strategies have been described for the treatment of complex abdominal wall defects. The component separation technique with transversus abdominis muscle release (TAR) allows adequate fasciomiocutaneos flaps mobilization to close the middle line and has low morbidity. Objective: to evaluate outcomes of TAR technique for the treatment of large incisional hernias. Material and methods: in the period between March 2013 and April 2017, all consecutive patients with TAR procedures with a minimum follow-up of 6 months were included. Demographics, operative and postoperative variables were analyzed. Results were expressed in medians. Results: a total of 14 TAR procedures were performed. Ten patients were men. The age was 60 (35-81) years and BMI was 28,2 kg/m². Seventy percent of the patients had at least two previous surgeries. CT scan was performed before surgery in all patients. Tanaka index was 24,5%. Seven patients had ASA < III. The defect size was 480 (224-720) cm² and mesh size 900 (500 - 1050) cm². Operative time was 248 (180 -341) minutes and hospital stay 3 (2-4) days. Two patients had surgical site infection. Postoperative follow-up was 20 (6-44) months. There was no recurrences. Conclusion: TAR technique is safe and reliable for the repair of large abdominal wall defects with low morbidity and recurrence rates. Therefore it should be taken into account in the surgical armamentarium.

PAHs exposure and occupational health risk assessment of workers in coal tar pitch factory / 中华劳动卫生职业病杂志

Objective@#To Investigation of industrial sites involving coal tar pitch to detect PAHs in the occupational environment and to assess their occupational health risks to workers.@*Methods@#Taking coal tar pitch enterprises as the research object, and making the occupational health field investigation and inspection. Detecting the 16 PAHs in the samples by high performance liquid chromatography (HPLC) , analyze the PAHs exposed dose of the workers in the place, and using cumulative toxic equivalent quantity method, loss of life expectancy method and carcinogenic risk factor method to assessment worker's occupational health risk.@*Results@#In the 15 posts involved, Some workers' exposed to the total concentration of PAHs is higher than others, the maximum exposure concentration is 1931.45ng/m3. There are different hazard risk levels in different working post due to different processes.The lifetime risk of workers is significantly higher than the acceptable range. Some workers has higher carcinogenic risk and workers' life expectancy loss is up to 1033.95 hours.@*Conclusion@#Part of the coal tar pitch workers exposed to higher concentrations of PAHs, and beyond the occupational exposure limits.And there is a high occupational health risk due to high exposure to PAHs.

Application of TAR cloning in microbial secondary metabolites / 中国药科大学学报

@#Microbial secondary metabolites have been a major source for drug discovery and development due to their structural novelty and diversity. Microbial secondary metabolites, typically encoded by specific biosynthetic gene cluster(BGC), are non-essential for the growth and propagation of the microbes. Despite the abundant existence of microbes, the majority of them are unculturable under laboratory conditions. Moreover, given that most of the BGCs from culturable microbes are silent, the discovery of novel microbial secondary metabolites has been hampered. Recently, the heterologous expression of BGCs has become an attractive approach to discover various microbial secondary metabolites, among which TAR-based heterologous expression is one of the important tools. This review summarized the principle of TAR, the applications and the advanced strategies of TAR-based methods for heterologous expression of secondary metabolites, which may help the advancements of drug discovery and development from microbial sources.

Research Progress of the Relationship between the Expression of TAR DNA-binding Domain Protein 43 and Brain Injury / 法医学杂志

TAR DNA-binding domain protein 43 （TDP-43） is a highly conserved and widely expressed nuclear protein. Nowadays, the expression of TDP-43 can be found in most neurodegenerative diseases such as Alzheimer's disease, which makes it become a neurodegenerative disease associated marker protein. From the current research status at homeland and abroad, and around the relationship between the expression of TDP-43 and brain injury, this article emphatically probes into the specific expression and function of TDP-43 in acute and chronic brain injury based on the knowledge of its biological characteristics, which aims to explore the feasibility for determining the cause of death and the injury and disability situations by TDP-43 in forensic pathology.

Research of relationship between occupational exposure to coal tar pitch workers and cytogenetic damage / 中华劳动卫生职业病杂志

Objective@#To investigate the effect of coal tar pitch occupational exposure on the cytogenetic damage.@*Methods@#In July 2015, 691 workers exposed to coal tar pitch were selected as contact group. The administrative and the support crew 201 cases were selected as control group. Detect the tail DNA% and tail moment in peripheral blood lymphocyte as DNA damage degree by single cell gel electrophoresis (SCGE) . Detect the concentration of the metabolic product in urine by HPLC/MC as exposure levels.@*Results@#The contact group were significantly higher than the control group in tail DNA% (contact group14.44%, control group 11.17%) and olive tail moment (contact group 2.85 μm, control group 1.95 μm) . The smoking one (contact group18.51%, control group13.43%) were significantly higher than the group not smoking (contact group12.69%, control group 11.71%) in tail DNA%. The coal tar pitch content in the air of workplace have correlation with worker, stail DNA% (rs=0.10) and olive tail moment (rs=0.11) .@*Conclusion@#Occupational exposure to coal tar pitch and smoking can cause cytogenetic damage to workers.

Analysis of inner or extra-exposure for worker in carbon and coal tar process industries / 中华劳动卫生职业病杂志

Objective@#To evaluate the inner or extra-exposure of workers in carbon and coal tar process industries.@*Methods@#In May 2015, 271 exposure and 75 non-exposure workers in two of carbon and coal tar process industries in Shandong province were selected. Coal tar pitch (CTP) was detected according to the methods of GB17054-1997. The 1-OH-Nap、2-OH-Nap、1-OH-Pyr in urine were detected by high performance liquid Chromatography (HPLC) .@*Results@#The concentration of 1-OH-Nap、2-OH-Nap、1-OH-Pyr in urine of exposure group were significant higher than that of control group (P<0.05) , respectively. The urine concentration of 1-OH-Nap、2-OH-Nap、1-OH-Py in contact group tar and asphalt were 12.20, 12.55, 7.08 and 10.62, 8.73, 3.07 μg/gCr, and the concentration of them were 7.25, 8.54, 3.00 μg/gCr in container handle workers. Significant positive correlations were found between high or median CTP exposure and the urine concentration of 1-OH-Nap、2-OH-Nap、1-OH-Pyr in exposure groups (P<0.05) .@*Conclusions@#The inner or extra exposure level is relative and different for different type of work in these carbon and coal tar process industries.

Investigation and analysis on occupational hazards in a carbon enterprise / 中华劳动卫生职业病杂志

Objective@#To explore occupational-disease-inductive in a carbon enterprise workplace and personnel occupational health examination, providing the basis for occupational disease prevention and control of the industry.@*Methods@#Field occupational health survey and inspection law were used to study the the situation and degree of occupational disease hazards in carbon enterprise from 2013 to 2015.Occupational health monitoring was used for workers, physical examination, detection of occupational hazard factors and physical examination results were analyzed comprehensive.@*Results@#Dust, coal tar pitch volatiles, and noise in carbon enterprise were more serious than others. Among them, the over standard rate of coal tar pitch volatiles was 76.67%, the maximum point detection was 1.06 mg/m3, and the maximum of the individual detection was 0.67 mg/m3. There was no statistical difference among the 3 years (P>0.05) . There were no significant differences in the incidence of occupation health examination, chest X-ray, skin audiometry, blood routine, blood pressure, electrocardiogram between 3 years (P>0.05) , in which the skin and audiometry abnormal rate was higher than 10% per year.@*Conclusion@#Dust, coal tar, and noise are the main occupational hazard factors of carbon enterprise, should strengthen the corresponding protection.

A association of genetic polymorphisms of XRCC1 and damage levels in workers exposed to coal tar pitch / 中华劳动卫生职业病杂志

Objective@#To study the relationship between XRCC1 gene polymorphism and DNA damage in peripheral blood lymphocytes of workers exposed to coal tar pitch.@*Methods@#203 coal tar asphalt device operation area workers (exposure group) and 76 logistics management personnel (control group) as the research ob-ject, determination of 1-hydroxypyrene concentrations in the urine as polycyclic aromatic hydrocarbons expo-sure dose, using the alkaline comet assay evaluation a peripheral blood lymphocyte DNA damage degree, using TaqMan MGB real time PCR method to detect XRCC1 gene 3 loci (XRCC1-194, XRCC1-280 and XRCC1-399) single nucleotide polymorphism.@*Results@#No significant differences was observed in age, sex, smoking and alco-hol consumption between the two groups (P>0.05). The level of 1-OHP in the exposed group was significantly higher than that in the control group (1.27±0.93 μg/g creatinine) (P<0.05). The comet Olive tail moment level (3.21±0.93) in the peripheral blood lymphocytes of the exposed group was significantly higher than that in the control group (0.94 ± 0.39) (P<0.05). There was no significant difference in genotype distribution of XRCC1-194, XRCC1-280 and XRCC1-399 between the two groups (P>0.05). There was a significant correlation be-tween the XRCC1-280 locus gene polymorphism and comet Olive tail moment in the exposure group (P<0.05) af-ter adjustment for sex, age, smoking rate, drinking rate, length of service and urinary 1-OHP concentration. The comet Olive tail moment level of GG individuals carrying wild homozygous genotype was significantly lower than that of individuals carrying heterozygous genotype GA and carrying mutant homozygous genotype AA (P< 0.05) , and the difference was statistically significant (P<0.05) The comet Olive tail moment level of heterozy-gous genotype GA was significantly lower than that of genotype AA with mutational homozygous genotype AA (P<0.05) , and the difference was statistically significant (P<0.05).@*Conclusion@#Arg280His locus polymor-phism of XRCC1 gene may influence the DNA damage level of peripheral blood lymphocytes induced by occupational exposure to coal tar pitch.

Detection of Ferric Iron Based on Fluorescence Quenching Effect of N-doped Carbon Quantum Dots / 分析化学

The photoluminescence properties of carbon quantum dots depend on their size and the properties of surface functional groups. The N-doped carbon dots ( using small molecular ethylenediamine ) with high quantum yield and excellent dispersibility were synthesized by one-step hydrothermal method with biomass tar that was generated in the reductive smelting process as a precursor. Rapid and accurate Fe3+ detection based on the selective fluorescence quenching effect of N-doped carbon quantum dots was achieved. The results showed that the as-synthesized N-doped carbon quantum dots were regular spherical, uniform in size with an average particle size of 2. 64 nm with a quantum yield of 26. 1%, and the crystal lattice spacing was 0. 25 nm, corresponding to the ( 100 ) facet of graphitic carbon structure. The functional groups on the surface of N-doped carbon quantum dots could interact with Fe3+ to form complex compound by coordination, leading to the fluorescence quenching effect. Fluorescence emission ratios kept a linear relationship with the concentrations of Fe3+ in the range of 0. 23-600 μmol/L with the detection limit of 230 nmol/L.

Detection of Ferric Iron Based on Fluorescence Quenching Effect of N-doped Carbon Quantum Dots / 分析化学

The photoluminescence properties of carbon quantum dots depend on their size and the properties of surface functional groups. The N-doped carbon dots ( using small molecular ethylenediamine ) with high quantum yield and excellent dispersibility were synthesized by one-step hydrothermal method with biomass tar that was generated in the reductive smelting process as a precursor. Rapid and accurate Fe3+ detection based on the selective fluorescence quenching effect of N-doped carbon quantum dots was achieved. The results showed that the as-synthesized N-doped carbon quantum dots were regular spherical, uniform in size with an average particle size of 2. 64 nm with a quantum yield of 26. 1%, and the crystal lattice spacing was 0. 25 nm, corresponding to the ( 100 ) facet of graphitic carbon structure. The functional groups on the surface of N-doped carbon quantum dots could interact with Fe3+ to form complex compound by coordination, leading to the fluorescence quenching effect. Fluorescence emission ratios kept a linear relationship with the concentrations of Fe3+ in the range of 0. 23-600 μmol/L with the detection limit of 230 nmol/L.

Capillary Electrophoresis Detection of Hydrogen Peroxide by Using Titanium Ion and 4-(2-thiazolylazo)resorcinol

A novel method for the detection of hydrogen peroxide in aqueous solution was developed via reaction between H₂O₂, trivalent titanium ion (Ti³⁺) and 4-(2-thiazolylazo) resorcinol (TAR), resulting in a ternary complex with a maximum UV absorbance at 530 nm. The CE detection of H₂O₂ was fast, sensitive and cost-effective without pretreatment procedures. H₂O₂ was detected within 15 min at 1 to 100 µM range with the lowest detection limit at 1.0 µM. Under the optimized CE conditions, the concentration of H₂O₂ in coffee or tea extract was quantitatively determined. Our results show that CE detection of the ternary complex of H₂O₂-Ti³⁺-TAR has potential applications for the detection of H2O2 in aqueous sources.

Isolation and characterization of phenol degrading yeasts from wastewater in the coking plant of Zarand, Kerman

Abstract Phenol and phenolic compounds are environmental pollutants present in industrial wastewaters such as coal tar, oil refineries and petrochemical plants. Phenol removal from industrial effluents is extremely important for the protection of environment. Usually, phenol degradation is carried out by physicochemical methods that are costly and produce hazardous metabolites. Recently, phenol biodegradation has been considered. Yeasts are the most important phenol biodegraders. In this study, the phenol-degrading yeast from environmental samples (soil and wastewater) was isolated from the coking plant of Zarand, Kerman. Then total heterotrophic yeasts were counted. The soil samples had higher rates of yeast degrader, in comparison to wastewater samples. After three passages, four yeasts (K1, K2, K7 and K11) that had the highest growth rate were selected for further study. Also, these yeasts were able to remove phenol measured by Gibbs reagent. The effect of four different concentrations of phenol (50, 125, 200 and 275) mg L−1 was measured and three degradation patterns in these yeasts were observed. The hydrophobicity and emulsification activity were measured in all eleven yeasts. Finally, strong yeasts in phenol degrading yeasts were identified by molecular method using amplification of 18S rRNA gene region. The sequencing results showed that these isolated yeasts belonged to Candida tropicalis strain K1, Pichia guilliermondii strain K2, Meyerozyma guilliermondii strain K7 and C. tropicalis strain K11.

La Cascada de la adherencia a la atención integral al tratamiento antirretroviral en Guatemala y factores institucionales que influyen en su evaluación / The Cascade of the integral attention to the treatment antiretroviral treatment in Guatemala and institutional factors that influence your evaluation

Introducción: La implementación de la terapia antirretroviral (TAR) en Centroamérica ha tenido un desarrollo diferente en cada país. En Guatemala en la última década, se ha ampliado sus coberturas de manera importante y por el apoyo inicial de Médicos sin Fronteras y luego con los Recursos del Fondo Mundial de Lucha contra el Sida, Malaria y Tuberculosis, y el progresivo aumento de la inversión por parte del Ministerio de Salud y el Seguro social, el acceso al TAR es una realidad. Sin embargo, con los conceptos de acceso universal al diagnóstico y tratamiento, y buscando llegar a los estándares propuestos por la Cascada o Continuum de Adherencia a la Atención integral y el TAR, una evaluación de su estado actual, se hizo necesaria. Objetivos: Desarrollar un diagnóstico de situación del estado de la adherencia al tratamiento ARV y establecer los componentes de la cascada de atención, relacionados con la adherencia en Guatemala. Métodos: Como parte Proyecto CAMPLUS y con la coordinación de Proyecto Capacity-USAID Guatemala, con expertos locales, se desarrolló una guía para establecer el cumplimiento de TAR, utilizando la carga viral y para documentar la cascada de adherencia al tratamiento del VIH, en Guatemala...(AU)

Reform of information literacy education according to The framework for information literacy in higher education of America / 中华医学图书情报杂志

After a description of the status quo of information literacy education in domestic colleges and universi-ties and the information literacy courses offered in University of Illinois according to The framework for information literacy in higher education, the information literacy courses offered in University of Illinois according to The frame‐work for information literacy in higher education were analyzed in aspects of their teaching target, teaching contents and teaching methods, with suggestions put forward for domestic information literacy education, including construc-tion of information literacy courses system and implementation of flexible teaching methods.

Nas malhas da rede com Clarice Lispector... aproximações entre a escrita clariceana e a escrita na TAR / In the meshes of the network with Clarice Lispector... approaches between Clarice's writings and the writings in ANT / En las Mallas de la Red con Clarice Lispector... similitudes entre los escritos de Clarice e os escritos en la TAR

Neste artigo pretendemos trazer ditos e escritos de Clarice Lispector para uma interlocução com a Teoria Ator-Rede (TAR), de Bruno Latour. Em nossos encontros, formais ou informais na UERJ, percebemos que com frequência a esta escritora recorremos nas referências e ilustrações ao estudo da TAR a que temos nos dedicado na pós-graduação em Psicologia Social (PPGPS). De acordo com a própria Clarice, não tivemos a presunção de explicá-la ou interpretá-la - apenas segui-la e explicitar as conexões com o que faz pesquisador da TAR ao compor seu texto. Dedicamo-nos a ampliar o diálogo entre nós e Clarice, percorrendo prazerosamente os meandros de sua inspiradora escrita através da maneira singular e plena de afetos com que articula atores humanos e não humanos, criando uma relação de intimidade com o leitor.

In this paper we intend to bring Clarice Lispector's words and texts to a dialogue with Bruno Latour's Actor-Network Theory (ANT). During our meetings at UERJ we realized that we frequently made a link within Lispector's thoughts and Latour's.We didn't have the intention of giving an explanation neither an interpretation of her writings - we've just followed her steps towards a connection with ANT. It was for us a pleasure to go in search of her paths, to the peculiar and full of feelings way in which she could manage human actors and non human actors in an intimate relationship with the reader.

En este artículo pretendemos traer palabras y textos de Clarice Lispector para un dialogo con la Teoría Actor-Red (TAR) de Bruno Latour. En nuestros encuentros formales e informales en la UERJ, percibimos que con frecuencia recurrimos a esta escritora para traer referencias e ilustraciones al estudio de la TAR, a la que nos hemos dedicado en el Programa de Pos Graduación en Psicología Social (PPGPS). Estando de acuerdo con la propia Clarice, no tuvimos la intención de explicarla o interpretarla - solamente de seguirla, explicitando las conexiones con la TAR. Nos dedicamos a ampliar nuestro diálogo con Clarice, recorriendo placenteramente los recovecos de sus escritos a través de la singular manera, llena de afectos, con que articula actores humanos y no humanos, creando una relación de intimidad con o lector.