ABSTRACT
Objective:To explore the correlation between the environmental factors and primary metabolite of Taxus mairei (Le-mee et Levl.) S.Y. Hu ex Liu. and to provide experimental basis for the further discussion on the biological characteristics,develop-ment and utilization of Taxus mairei (Lemee et Levl.) S.Y. Hu ex Liu.. Methods:Correlation analysis and path analysis were used to explore the correlation of temperature, sunshine duration and precipitation with the relative primary metabolite content by SPSS 19.0. Results:The environmental factor with the biggest direct contribution to the absolute value of relative primary metabolite content was average monthly temperature. The average monthly temperature by month cumulative sunshine time showed the largest influence on the relative primary metabolite content. Conclusion:The relationship between temperature and sunshine time is much close, and the most obvious factor on the primary metabolite accumulation is temperature changes. This research provides theoretical basis and certain guiding significance for the further development,utilization and studies on harvesting,processing, using and explaining the biological characteristics of Taxus mairei (Lemee et Levl.) S.Y. Hu ex Liu..
ABSTRACT
This article was aimed to study enhancing efficacy and reducing toxicity on anti-tumor from total flavonoids and total polysaccharides in Taxus mairei compatibility with taxol. MTT assay was performed to analyze the cell proliferation and apoptosis of 4T1, A549, MCF-7 tumor cells after treatment of total flavonoids and total polysaccharides in T. mairei combined with taxol at different concentrations. Isobolograms was used to investigate the behavior of combination therapy. Therapy experiments were conducted on ICR mice bearing S180 sarcoma. The tumor weight was measured to calculate inhibition rate according to uniform design. Blood cells indicators were detected by blood cell analyzer. The results showed that total polysaccharides and total flavonoids can enhance the inhibition effect on 4T1, A549 cells by taxol. Total polysaccharides can enhance the inhibition effect of taxol to MCF-7 in vitro, and S180 sarcoma-bearing mice in vivo. It reduced the toxic effects of anti-tumor effect of taxol in vivo. It was concluded that taxol can achieve the effect of enhancing efficacy and reducing toxicity through combining with total flavonoids and total polysaccharides in T. mairei.
ABSTRACT
[Objective] To determine dormancy mechanism of Taxus mairei(Lemee et Levl.) S.Y.Hu ex Liu Seeds,the best method of dormancy breaking.[Method]The seeds were handled by lotic water or excitatory autacoid or cold-warm-cold stratification treatment or cold-warm-cold stratification treatment with excitatory autacoid,then compare withgermination inhibition of Brassica campestris seeds handling with water solution from the seeds after four dormancy breaking methods,to dertemine the best dormancy breaking method.[Conclusion] The best damancy breaking method was cold(4℃)—warm(23℃)—cold(4℃) stratification treatment with 0.05% gibberellic acid for 120 days.