Ação dos desinfetantes sobre a adesão e biofilme consolidado de staphylococcus spp / Action of products based on chlorhexidine and iodine for the adhesion and consolidated biofilm of staphylococcus spp isolated from milk

Staphylococcus spp. são reconhecidos como importantes causadores de mastites em rebanhos leiteiros. Esses micro-organismos têm a capacidade de produzir uma estrutura denominada biofilme, que é responsável pela sobrevivência e muitas vezes pela resistência a ação de produtos desinfetantes e as demais condições adversas. Neste trabalho avaliou-se a ação de dois produtos pós-dipping a base de iodo (0,7%) e clorexidine (2,0%) sobre a adesão de Staphylococcus coagulase positiva (SCP) e Staphy-lococcus coagulase negativa (SCN) isolados de casos de mastite subclínica, e também sobre biofilmes pré formados a partir destes isolados. Os produtos testados apresentaram uma alta redução na taxa de adesão de todos os isolados. No entanto, a ação sobre os biofilmes consolidados só foi estatisticamente significativa sobre os SCN. Assim, ressalta-se a importância dos programas sanitários a fim de prevenir a formação de biofilmes e diminuir as fontes de contaminação da glândula mamaria em sistemas de produção leiteira.

Staphylococcus spp. are recognized as important causes of mastitis in dairy herds. These microorganisms have the ability to produce a structure called biofilm, which is responsible for the survival and often for resistance to the action of disinfectants and other harsh conditions. In this paper we evaluate the action of two teat dip products based on iodine (0,7%) and chlorhexidine (2,0%) on the adhesion of coagulase-positive staphylococci (CPS) and coagulase negative staphylococci (CNS) from subclinical mastitis cases, and also on its consolidate biofilm. The products tested showed a high reduction on the adhesion of all isolates. However, action on the consolidate biofilm was significant for CNS only, and unsatisfactory for CPS. Thus, it emphasizes the importance of health programs to prevent biofilm formation and reduce the sources of contamination of the mammary gland in dairy production systems.

Factors Affecting Iodine Content in Dairy Cow’s Milk – a Review.

Recently, milk has been suggested as one of the main sources of iodine for human intake. However, there is the potential for problems resulting from excessive amounts of iodine in cow milk. Supplementary feed iodine increases milk iodine and is apparently the cause of many of the high milk iodine values. Iodine teat dips and sanitizers also contribute to increased milk iodine values. Variability of these practices may result in variation of iodine concentrations in milk. In addition, the transportation and processing phases of milk production, where iodophor sanitizers are used, season, geographic region, stage of lactation and iodine antagonists in feeds, may influence milk iodine concentration. It seems that Iodine concentration in milk should be monitored, regularly.