ABSTRACT
Objective To discuss the effect of polysaccharides from Tegillarca granosa as an adjuvant on pcDNA3.1?Sj26GST vaccine against Schistosoma japonicum infection. Methods Sixty SPF BALB/c mice were divided into four groups randomly(15 mice each group),including a control group,a polysaccharides group,a vaccine group,and a vaccine plus poly?saccharides group. In the 0,2th and 4th week of the experiment,the mice in the above four groups were immunized for 3 times with 100μl PBS,100μg polysaccharides from Tegillarca granosa,100μg Sj26GST vaccine,and 100μg Sj26GST vaccine plus equivalent polysaccharides,respectively. Two weeks after the last immunization,all the mice were infected with 40 ± 1 Schistosoma japonicum cercariae through the skin of the abdomen. After the infection for 6 weeks,all the mice were sacrificed, and their serums,livers and the adult worm of Schistosoma japonicum in them were collected,the specific sera IgG antibodies were detected by ELISA,and the worm reduction rates and egg reduction rates in the liver were calculated. Results Six weeks after the infection,the IgG antibody levels of the mice in the vaccine group and the vaccine plus polysaccharides group were 18.26 ± 0.42 mg/ml and 20.21 ± 0.89 mg/ml respectively,the difference between them were statistically significant,and both of the IgG levels of the above groups were significantly higher than those in the control(both P<0.05). The worm reduction rate and egg reduction rate in the vaccine group were 28.60%and 35.84%,respectively,and the rates in the vaccine plus polysac?charides group were 38.04% and 49.74%,respectively,the differences between the worm reduction rates and egg reduction rates were both statistically significant(both P<0.05). Meanwhile,the two rates in the two above groups were all significantly higher than those in the control group(all P<0.01). Conclusion Polysaccharides from Tegillarca granosa using as an adju?vant can increase the protection effect of pcDNA3.1?Sj26GST vaccine against Schistosoma japonicum infection.
ABSTRACT
Objective To investigate the influence of a new marine preparation(Haishengsu) isolated from Tegillarca granosa on NSCLC patients after chemotherapy.Methods Eighty-three patients with NSCLC were randomly divided into two groups.Forty-two patients,in the treatment group were received the injection 2.4mg+0.9%NaCl 250mL,iv.drip from 1 to 15 days while they were received the routine chemotherapy of MVP.Forty-one patients in the control group were received only the chemotherapy of MVP.Twenty-eight days were considered as one treatment cycle and each patient was received two or more consecutive treatment cycles respectively.Results The gastrointestinal tract reactions in treatment group were alleviated obviously with increased food-intake and body weight gain.Moreover the KPS value and the curative effect in treatment group were higher than those in control group.Conclusion Treatment of Haishengsu injection plus MVP chemotherapy improves the curative effect and life quality of the NSCLC patients without marked adverse effects.
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Aim To study the infuences of Haishengsu extracted from Tegillarca granosa on cultured tumor cells of lung cancer, breast cancer and carcinoma of stomach from human in vitro and its preventive antitumor effect on transplanted tumor in mice in vivo. Methods The inhibitory effect of Haishengsu on tumor cells proliferation was assayed by MTT method, and transplant tumor models of sarcoma 180 (S 180), Ehrlich ascites carcinoma (EAC), and hepatoma (Heps) were used. Before and after transplantation, different doses of Haishengsu were given to the mice for 10 days. The inhibition rates and life span of the mice were calculated. Results Haishengsu at concentrations of 0.5%～2.0% inhibited the proliferation of tested tumor cells in vitro,especially on lung cancer cells with a dose dependent manner (r= 0.974,P
ABSTRACT
Superoxide dismutase(SOD) was purified from Tegillarca granosa and the effects of tributyltin chloride(TBTCl) on the enzyme activity of the SOD were analyzed at different conditios. The results showed that the effects of TBTCl on SOD activity were more ohvious at room temperature than at four degrees centigrade. 58. 1 % of the enzyme activity was reserved after SOD was incubated with 200?g?mL-1 of TBTC1 for 72 hours at room temperature, while 90. 9% of the enzyme activity was reserved at four degrees centigrade. The enzyme activity of SOD was reduced with the increase of TBTC1 concentration and interaction time. TBTCl could reduce heat-resisting, acid-resisting and alkali-resisting abilities of SOD. With TBTCl concentration increase,SOD isozyme bands in the graph of polyacrylamide gel electrophoresis were getting weaker.