ABSTRACT
Objective@#This study investigated the effects of interleukin-1β (IL-1β) on human synovial fluid-derived mesenchymal stem cells (hSFMSCs) in the temporomandibular joint.@*Methods @#hSFMSCs from synovial fluid samples of temporomandibular disorder (TMD) patients were cultured in vitro. hSFMSCs were divided into three groups with different concentrations of rhIL-1β in complete medium (α-MEM cell culture medium + 10% FBS + 1× GlutaMAX): 0 ng/mL IL-1β group, 1 ng/mL IL-1β group and 10 ng/mL IL-1β group. Changes in the rate of colony formation, growth curve, cell cycle and apoptosis of hSFMSCs under IL-1β stimulation were evaluated. The osteogenic, adipogenic and chondrogenic potential of the cells were also determined using histochemical and real-time fluorescence quantitative PCR methods. @*Results @#No significant differences in growth or proliferation capacity were observed in any IL-1β-stimulated group in comparisons of the colony-formation rate (F = 0.665, P=0.548), growth curve (F=0.001, P=0.999), cell cycle (FG1=0.773, PG1=0.503; FS =0.636, PS =0.562) or apoptosis (F=0.196, P=0.827) of the cells. However, the multidifferentiation capacity of hSFMSCs was affected in the inflammatory environment. Mineralized nodule and lipid clusters decreased significantly, and the gene expression levels of runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), peroxisomal proliferative receptor G2 (PPARG2) and lipoprotein lipase (LPL) were suppressed significantly in IL-1β-mediated induction medium (P < 0.05). In general, cartilage pellets formed in all the IL-1β-mediated chondrogenic differentiation groups. The gene levels of sex-determining region Y-related high-mobility group box-9 (SOX9) and collagen II were decreased (P < 0.05), while that of matrix metalloproteinase 13 (MMP13) was increased significantly in the presence of IL-1β (P < 0.05). @*Conclusion@# IL-1β directly affects the multidifferentiation potential of hSFMSCs but not their cell growth or proliferation ability.
ABSTRACT
La condromatosis sinovial es una metaplasia cartilaginosa del tejido sinovial de las articulaciones. Es una enfermedad de etiología desconocida e infrecuente. Su localización en la articulación temporomandibular es más rara aún. Se describe el caso clínico de una paciente con diagnóstico de condromatosis sinovial de la articulación temporomandibular tratada con éxito mediante un abordaje quirúrgico combinado: externo y con endoscopios de 4 mm. Se realizó una revisión de la bibliografía sobre la información existente de la condromatosis sinovial.
Synovial chondromatosis is a cartilaginous metaplasia of the synovial tissue of joints. It is a disease of unknown etiology and infrequent. Its location in the temporomandibular joint is still more unusual. The case of a patient is described with a diagnosis of synovial chondromatosis of the temporomandibular joint successfully treated with a combined surgical approach: external and with 4 mm endoscopes. A review of the literature was conducted on the existing information on synovial chondromatosis.
A condromatose sinovial é uma metaplasia cartilaginosa do tecido sinovial das articulações. É uma doença de etiologia desconhecida e pouco frequente. A sua localização na articulação temporomandibular é ainda mais rara. Descreve-se o caso clínico de uma paciente com diagnóstico de condromatose sinovial da articulação temporomandibular tratada com sucesso mediante uma abordagem cirúrgica combinada: externa e com endoscópios de 4 mm. Realizou-se uma revisão da bibliografia sobre a informação existente da condromatose sinovial.
Subject(s)
Female , Humans , Aged , Chondromatosis, Synovial/diagnosis , Chondromatosis, Synovial/surgery , Endoscopy , Temporomandibular Joint/surgeryABSTRACT
Objective To analyze and compare the difference of the joint position between the Angle classⅡ division 2 malocclu-sion and individual normal occlusion by dental cone beam computed tomography (CBCT).Methods 23 patients with Angle classⅡdivision 2 malocclusion according with the experimental design were selected as the experimental group and 27 cases of individual normal occlusion as the control group.Each research subj ect was performed CBCT.The images in the oblique position perpendicular to the long axis of condyloid process were reconstructed by the Examvision software.Then the joint space was measured by the Au-toCAD software and and the positional relation of the condyloid process in the articular fossa was assessed by Pullinger analysis methods.The results were compared.Results 65.2% of the condylloid process in Angle classⅡ division 2 malocclusion is posteri-orly shifted,in individual normal occlusion,64.8% of the condylloid process is in the middle place.Conclusion The condylar posi-tion of Angle classⅡ division 2 malocclusion were abnormal relative to individual normal occlusion joint,which should be early cor-rected for inducing the joint to the normal position and avoiding the generation of the joint symptoms.