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Background: Hepatotoxicity ultimately leads to liver failure. Conventional treatment options for hepatotoxicity are limited and not safe. Aim: The present work has been designed to evaluate the hepatoprotective effects of ethanolic extract of the root of Tephrosia purpurea (Linn.) against thioacetamide-induced hepatotoxicity in experimental Wistar albino rats. Materials and Methods: The plant roots, T. purpurea, were collected from the local area of Jodhpur, Rajasthan, and verification was done by Botanical Survey of India (BSI), Jodhpur, Rajasthan, and a herbarium specimen was deposited in BSI with No. LMC/PM/PD-001. All other reagents and chemicals were of suitable analytical grade and were used as received. Results: On the basis of statistical analysis, both the doses (200 and 400 mg/kg b.wt) of the ethanolic extract of T. purpurea root shown significant hepatoprotective activity compare to negative control. The dose of 400 mg/kg b.wt showed better reduction in serum enzyme level compare to 200 mg/kg b.wt dose of the ethanolic extract of T. purpurea root. Results were determined by one-way analysis of variance (ANOVA non-parametric) followed by Dunnett’s test with P < 0.01 considered statistically significant. Conclusion: Based on the results obtained, it may be concluded that the ethanolic extract of T. purpurea root has a significant protective effect on liver injuries
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Objective: The objective of the present investigation was to find antimicrobial and MIC of endophytic fungi Fusarium sp. isolated from Tephrosia purpurea root. Methods: Well diffusion assay was performed to find out the antimicrobial activity and Resazurin dye reduction method was performed to find out MIC of the extract. Result: The extract showed the highest zone of inhibition of 22.66±0.57 mm, (Bacillus subtilis, MTCC-441) for Gram-positive bacteria and 20.66±0.57 mm, (E. coli, MTCC-443) for Gram-negative bacteria. Furthermore, the MIC of the extract was found to be (31.25 µg/ml-125 µg/ml). Conclusion: Hence, the endophytic fungi isolated from the Tephrosia purpurea root, i.e. Fusarium sp. showed good antimicrobial activity and hence can be used to find a novel drug.
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OBJECTIVE@#The present study is to determine the potential treatment effects of ethyl acetate fraction of Tephrosia purpurea Linn. leaves (EATP) against gout.@*METHODS@#Gout in experimental rats was induced with potassium oxonate at the dose of 250 mg/kg (intraperitoneal injection) for 7 consecutive days; EATP was administered 1 h after administration of the potassium oxonate on each day of experiment. Potassium oxonate was discontinued on the 8th day; thereafter allopurinol (10 mg/kg, p.o.) and EATP (200 and 400 mg/kg, p.o.) were continued until day 14. The uric acid level was measured from serum and urine during the experiment. Other biochemical parameters were assessed, including blood and urine creatinine, erythrocyte sedimentation rate, and total protein. Blood urea nitrogen, serum aspartate aminotransferase serum alanine aminotransferase and alkaline phosphatase were also measured. The blood was analyzed for levels of malondialdehyde and the antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Histopathological and radiological changes in the ankle of rats were observed after completion of the experiment.@*RESULTS@#EATP was able to decrease serum uric acid and creatinine level; it also reduced inflammation, oxidative stress and lysosomal enzyme level, which has a role in acute inflammation. EATP increased uric acid excretion through urine due to its uricosuric effect.@*CONCLUSION@#EATP lowered the serum uric acid level and increased the urine uric acid level through excretion, which is useful in the treatment of gout. Hence the EATP was found to be helpful in the treatment of gout.
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Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.
Subject(s)
Ascomycota , Colon , Fungi , Plants , Sequence Analysis , TephrosiaABSTRACT
Tephrosia purpurea (fabaceae) commonly known in sanskrit as ‘sharapunkha’ is highly branched ,herbaceous, suberect, perennial herb.widely grown in india. Whole plant and various parts of the plant are useful as ayurvedic medicines. Medicinal uses of drugs are tonic, laxative, diuretic, bronchitis, bilious febrile attack, boils, pimples, diarrhea, gonorrhea, rheumatism and cures disease of heart, spleen and blood. The pharmacological studies have shown that Tephrosia purpurea posses following biological activity such as antiulcer, antimicrobial, antibacterial, anti viral, anti asthmatic ,hepatoprotective, antihyperglycemic and antihyperlipidemia, immunomodulatory activity, antioxidant, wound healing property, antiallergic activity. A wide variety of phytochemicals are isolated from the plant Tephrosia purpurea which has concerned with their medicinal uses. The present review high lights the mainly phytochemistry and pharmacological activity of the plant.
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Tephrosia purpurea (L.) Pers. is popularly known as 'Sarapunkha' in classical Ayurvedic texts. It is a perennial plant belonging to the family Fabaceae, and occurs throughout the Indian subcontinent. T. purpurea is traditionally used to treat splenomegaly, cirrhosis, cough and cold, abdominal swelling and as an antidote in the Ayurvedic system of medicine. Phytochemical investigations indicate the presence of semiglabrin, pongamole, lanceolatins A and B, rutin, lupeol, and β-sitosterol. Flavonoids including (+)-tephrorin A and B, (+)-tephrosone, an isoflavone, 7, 4'-dihydroxy-3', 5'-dimethoxyisoflavone and a chalcone, (+)-tephropurpurin were isolated from the whole plant. Pharmacological activities of different parts of the plant reported include anti-inflammatory, antiulcer, antimicrobial, antioxidant, antiallergic, antidiabetic, hepatoprotective, antitumor and insect repellent activity. In the present review, the literature on the phytochemical and pharmacological investigations of Tephrosia purpurea (L.) Pers. are summarized to August, 2012.
Subject(s)
Animals , Humans , India , Medicine, Ayurvedic , Plant Extracts , Chemistry , Pharmacology , Tephrosia , ChemistryABSTRACT
The aim of this work was to study the larvicidal activity of Tephrosia purpurea (L) Pers. against the larvae of culex quinquefasiciatus. The preliminary laboratory trail was undertaken to determine the efficacy of petroleum ether and ethyl acetate extract of dried whole plant of Tephrosia purpurea belonging to the family Papilionaceae at various concentrations against the late third or early fourth instar larvae of culex quinquefasiciatus by following the WHO guidelines. The results suggest that 100% mortality. Petroleum ether and ethyl acetate extract of Tephrosia purpurea (L) Pers. was observed at 250ppm and 300ppm respectively. The results suggested that use of plants in insect control as an alternative method for minimizing the noxious effect of some pesticide compound on the environment. Thus the extract of Tephrosia purpurea delivers promising more selective and biodegradable agent.